LOCUS       sequence-379959-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express codon-optimized human MIGA2 full length with a C-terminal 
            DsRed tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, 
            Reinisch KM
  TITLE     Mitoguardin-2-mediated lipid transfer preserves mitochondrial 
            morphology and lipid droplet formation.
  JOURNAL   J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 
            10.1083/jcb.202207022. Epub 2022 Oct 25.
  PUBMED    36282247
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-12-5"; volume: "221(12):e202207022. doi"; pages:
            " 10.1083/jcb.202207022. Epub 2022 Oct 25"
COMMENT     Sequence Label: pLVX-hMIGA2-DsRed
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             24..657
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    704..829
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1326..1559
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1744..1788
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2051..2166
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2224..2527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2528..2731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2682..2702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2728..2752
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4634..5311
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     5032..5051
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5335..5834
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5370..5393)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5767..5786
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5855..6454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5855..5874)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6351..6371
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6468..7056
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6521..6541)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6939..6950)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7263..7896
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8025..8041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8025..8041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8038..8060)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8049..8065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8073..8079,8080..8097,8098..8103))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8118..8139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8256..8273)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8427..9015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8507..8526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9186..9977,9978..10046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9809..9828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10047..10151)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10199..10333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10236..10255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10290..10309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
       61 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
      121 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
      181 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
      241 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
      301 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
      361 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
      421 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
      481 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      541 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      601 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      661 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      721 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      781 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      841 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      901 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
      961 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1021 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1081 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1141 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     1201 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1261 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1321 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1381 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1441 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1501 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1561 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1621 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1681 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1741 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1801 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1861 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1921 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1981 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     2041 cggtatcgcc tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt
     2221 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2281 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2341 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2401 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2461 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2521 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2581 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2641 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2701 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     2761 acgccatcca cgctgttttg acctccatag aagacaccga ctctactaga ggatcgctag
     2821 cgctaccgga ctcagatctc gagctcaagc ttcgatggcg tttcgtcgtg cggaaggcac
     2881 cagcatgatc caggcgctgg cgatgaccgt ggcggagatt ccggttttcc tgtacaccac
     2941 ctttggtcag agcgcgttta gccaactgcg tctgaccccg ggcctgcgta aagtgctgtt
     3001 cgcgaccgcg ctgggtaccg ttgcgctggc gctggcggcg caccagctga agcgtcgtcg
     3061 tcgtcgtaag aagcaagtgg gcccggaaat gggtggcgag caactgggta ccgttccgct
     3121 gccgatcctg ctggcgcgta aagtgccgag cgttaagaaa ggctatagca gccgtcgtgt
     3181 gcaaagcccg agcagcaaaa gcaacgacac cctgagcggc atcagcagca ttgaaccgag
     3241 caagcacagc ggtagcagcc acagcgtggc gagcatgatg gcggttaaca gcagcagccc
     3301 gaccgcggcg tgcagcggcc tgtgggatgc gcgtggtatg gaggaaagcc tgaccaccag
     3361 cgatggcaac gcggaaagcc tgtacatgca gggtatggag ctgtttgagg aagcgctgca
     3421 gaagtgggag caagcgctga gcgttggtca acgtggcgac agcggtagca ccccgatgcc
     3481 gcgtgatggt ctgcgtaacc cggaaaccgc gagcgagccg ctgagcgagc cggaaagcca
     3541 gcgtaaagag ttcgcggaaa agctggagag cctgctgcac cgtgcgtatc acctgcaaga
     3601 ggaattcggc agcacctttc cggcggacag catgctgctg gatctggaac gtaccctgat
     3661 gctgccgctg accgagggta gcctgcgtct gcgtgcggac gatgaagaca gcctgaccag
     3721 cgaggatagc ttctttagcg cgaccgaact gttcgagagc ctgcagaccg gcgactatcc
     3781 gattccgctg agccgtccgg cggcggcgta tgaggaagcg ctgcaactgg tgaaagaagg
     3841 ccgtgttccg tgccgtaccc tgcgtaccga gctgctgggt tgctacagcg accaggattt
     3901 tctggcgaag ctgcactgcg tgcgtcaagc gttcgaaggt ctgctggagg ataaaagcaa
     3961 ccagctgttc tttggcaaag tgggtcgtca aatggttacc ggcctgatga ccaaggcgga
     4021 gaagagcccg aagggttttc tggagagcta cgaggaaatg ctgagctatg cgctgcgtcc
     4081 ggaaacctgg gcgaccaccc gtctggagct ggaaggtcgt ggcgtggttt gcatgagctt
     4141 ctttgacatc gttctggatt ttattctgat ggacgcgttc gaagatctgg agaacccgcc
     4201 ggcgagcgtg ctggcggttc tgcgtaaccg ttggctgagc gacagcttca aagaaaccgc
     4261 gctggcgacc gcgtgctgga gcgtgctgaa ggcgaaacgt cgtctgctga tggttccgga
     4321 tggtttcatc agccactttt acagcgtgag cgaacacgtg agcccggttc tggcgttcgg
     4381 ctttctgggt ccgaaaccgc agctggcgga agtgtgcgcg ttctttaagc accagattgt
     4441 tcaatacctg cgtgacatgt tcgacctgga taacgttcgt tataccagcc tgccggcgct
     4501 ggcggatgat atcctgcagc tgagccgtcg tcgtagcgag attctgctgg gttatctggg
     4561 tgtgccggcg gcgagcagcg cgggcgttaa cggtgcgctg ccgcgtgaaa acggtccgct
     4621 gggcgagctg caaatggaca acaccgagga cgtcatcaag gagttcatgc agttcaaggt
     4681 gcgcatggag ggctccgtga acggccacta cttcgagatc gagggcgagg gcgagggcaa
     4741 gccctacgag ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt
     4801 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggcctacg tgaagcaccc
     4861 cgccgacatc cccgactaca tgaagctgtc cttccccgag ggcttcacct gggagcgctc
     4921 catgaacttc gaggacggcg gcgtggtgga ggtgcagcag gactcctccc tgcaggacgg
     4981 caccttcatc tacaaggtga agttcaaggg cgtgaacttc cccgccgacg gccccgtaat
     5041 gcagaagaag actgccggct gggagccctc caccgagaag ctgtaccccc aggacggcgt
     5101 gctgaagggc gagatctccc acgccctgaa gctgaaggac ggcggccact acacctgcga
     5161 cttcaagacc gtgtacaagg ccaagaagcc cgtgcagctg cccggcaacc actacgtgga
     5221 ctccaagctg gacatcacca accacaacga ggactacacc gtggtggagc agtacgagca
     5281 cgccgaggcc cgccactccg gctcccagta accgcgactc tagataattc taccgggtag
     5341 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     5401 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     5461 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     5521 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     5581 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     5641 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     5701 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     5761 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     5821 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     5881 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     5941 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6001 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6061 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6121 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6181 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6241 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6301 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6361 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6421 cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat caacctctgg
     6481 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6541 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6601 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     6661 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6721 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6781 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6841 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6901 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6961 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7021 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     7081 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     7141 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     7201 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     7261 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     7321 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     7381 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     7441 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     7501 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     7561 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     7621 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     7681 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     7741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7861 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7921 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct
     7981 agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    10201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10321 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    10381 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    10441 tgaattattt tcattttaaa gaaattgtat ttgttaa
//
