LOCUS       sequence-379960-        9992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express codon-optimized human MIGA2 full length with a C-terminal 
            DsRed tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, 
            Reinisch KM
  TITLE     Mitoguardin-2-mediated lipid transfer preserves mitochondrial 
            morphology and lipid droplet formation.
  JOURNAL   J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 
            10.1083/jcb.202207022. Epub 2022 Oct 25.
  PUBMED    36282247
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-12-5"; volume: "221(12):e202207022. doi"; pages:
            " 10.1083/jcb.202207022. Epub 2022 Oct 25"
COMMENT     Sequence Label: pGK-hMIGA2-DsRed
FEATURES             Location/Qualifiers
     source          1..9992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     327..350
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    415..530
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(808..852)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1037..1270
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1760..1885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1932..2112
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2113..2339)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_signal     2460..2610
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   2698..2800
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      2838..3218
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2925..2944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3135..3156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3266..4060)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3266..4060)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3377..3396)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3987..4006
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      4472..5060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4961..4980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5214..5231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5389..5510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5400..5419)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5454..5473
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5582..5815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5825..6412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5930..5941
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6339..6359
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6425..7024)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6508..6528)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7005..7024
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     rep_origin      7025..7160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7030..7327)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             complement(7345..7371)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(7378..8055)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     complement(7638..7657)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(join(9890..9992,1..385))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9938..9957)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
       61 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
      121 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
      181 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
      241 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
      301 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
      361 cttgggaaaa gcgcctcccc tacccggtag aatttctagg ttcgaaaaac ccgaaaattt
      421 tgaatttttg taatttgttt ttgtagttct ttagtttgta tgtctgttgc tattatgtct
      481 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa ttcgaatccc
      541 aagctttcga taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc
      601 tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt
      661 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
      721 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
      781 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
      841 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
      901 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
      961 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     1021 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     1081 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     1141 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     1201 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     1261 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     1321 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     1381 atgtccctca tatctcctcc tccaggtctg aagatcagcg ggcttgctgt gcggtggtct
     1441 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     1501 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     1561 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     1621 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     1681 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     1741 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     1801 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     1861 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     1921 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     1981 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     2041 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     2101 ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg
     2161 caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg
     2221 atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc
     2281 atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat
     2341 tttcgctacc ttaggaccgt tatagttacg tcaggtggca cttttcgggg aaatgtgcgc
     2401 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagagta
     2461 ttacgcgcta tgagtaacac aaaattattc agatttcact tcctcttatt cagttttccc
     2521 gcgaaaatgg ccaaatctta ctcggttacg cccaaattta ctacaacatc cgcctaaaac
     2581 cgcgcgaaaa ttgtcacttc ctgtgtacac cggcgcacac caaaaacgtc acttttgcca
     2641 catccgtcgc ttacatgtgt tccgccacac ttgcaacatc acacttccgc cacactacta
     2701 cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa actccacccc ctcattatca
     2761 tattggcttc aatccaaaat aaggtatatt attgatgatg ttaattaaga attaattcga
     2821 tcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2881 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     2941 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3001 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3061 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3121 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3181 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3241 gatttaacaa aaattttaac aaaattcaga agaactcgtc aagaaggcga tagaaggcga
     3301 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3361 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3421 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3481 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     3541 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3601 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3661 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3721 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3781 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     3841 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     3901 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     3961 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     4021 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     4081 tctcttgatc agagcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     4141 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt
     4201 cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta
     4261 cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca
     4321 tccggggtca gcaccgtttc tgcggactgg ctttctacgt gaaaaggatc taggtgaaga
     4381 tcctttttga taatctcatg gccaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4441 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4501 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4561 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4621 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4681 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4741 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4801 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4861 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4921 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4981 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5041 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5101 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5161 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5221 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     5281 gtatttcaca ccgacatccg ttggccgatt cattaatgca gacccataat acccataatg
     5341 ccatttcatt acctctttct ccgcacccga catagatgcc agacatgata agatacattg
     5401 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5461 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc
     5521 tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta
     5581 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     5641 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     5701 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     5761 ccagtacaag caaaaagcag atcttgtatt cgttgggagt gaattagccc ttccatccgg
     5821 acaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgagacgcg
     5881 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     5941 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     6001 atggaaagga cgtcagcttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     6061 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     6121 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     6181 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     6241 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     6301 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     6361 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga tttccggaac
     6421 gcgttcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     6481 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     6541 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
     6601 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     6661 cgggctcctt gggccggtgc ggcgccagga gcccttccat ctgttgctgc gcggccagcc
     6721 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
     6781 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
     6841 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     6901 ggcggtccgg gtccacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     6961 tgcgtacggc ccgagggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     7021 tcatggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     7081 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     7141 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     7201 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     7261 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7321 ccacaccacg cgtgtttaaa ctcaagcgta gtctgggacg tcgtatgggt actcgagtta
     7381 ctgggagccg gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg tgtagtcctc
     7441 gttgtggttg gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca gctgcacggg
     7501 cttcttggcc ttgtacacgg tcttgaagtc gcaggtgtag tggccgccgt ccttcagctt
     7561 cagggcgtgg gagatctcgc ccttcagcac gccgtcctgg gggtacagct tctcggtgga
     7621 gggctcccag ccggcagtct tcttctgcat tacggggccg tcggcgggga agttcacgcc
     7681 cttgaacttc accttgtaga tgaaggtgcc gtcctgcagg gaggagtcct gctgcacctc
     7741 caccacgccg ccgtcctcga agttcatgga gcgctcccag gtgaagccct cggggaagga
     7801 cagcttcatg tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtactg
     7861 gaactggggg gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcacctgcag
     7921 cttggcggtc tgggtgccct cgtagggctt gccctcgccc tcgccctcga tctcgaagta
     7981 gtggccgttc acggagccct ccatgcgcac cttgaactgc atgaactcct tgatgacgtc
     8041 ctcggtgttg tccatttgca gctcgcccag cggaccgttt tcacgcggca gcgcaccgtt
     8101 aacgcccgcg ctgctcgccg ccggcacacc cagataaccc agcagaatct cgctacgacg
     8161 acggctcagc tgcaggatat catccgccag cgccggcagg ctggtataac gaacgttatc
     8221 caggtcgaac atgtcacgca ggtattgaac aatctggtgc ttaaagaacg cgcacacttc
     8281 cgccagctgc ggtttcggac ccagaaagcc gaacgccaga accgggctca cgtgttcgct
     8341 cacgctgtaa aagtggctga tgaaaccatc cggaaccatc agcagacgac gtttcgcctt
     8401 cagcacgctc cagcacgcgg tcgccagcgc ggtttctttg aagctgtcgc tcagccaacg
     8461 gttacgcaga accgccagca cgctcgccgg cgggttctcc agatcttcga acgcgtccat
     8521 cagaataaaa tccagaacga tgtcaaagaa gctcatgcaa accacgccac gaccttccag
     8581 ctccagacgg gtggtcgccc aggtttccgg acgcagcgca tagctcagca tttcctcgta
     8641 gctctccaga aaacccttcg ggctcttctc cgccttggtc atcaggccgg taaccatttg
     8701 acgacccact ttgccaaaga acagctggtt gcttttatcc tccagcagac cttcgaacgc
     8761 ttgacgcacg cagtgcagct tcgccagaaa atcctggtcg ctgtagcaac ccagcagctc
     8821 ggtacgcagg gtacggcacg gaacacggcc ttctttcacc agttgcagcg cttcctcata
     8881 cgccgccgcc ggacggctca gcggaatcgg atagtcgccg gtctgcaggc tctcgaacag
     8941 ttcggtcgcg ctaaagaagc tatcctcgct ggtcaggctg tcttcatcgt ccgcacgcag
     9001 acgcaggcta ccctcggtca gcggcagcat cagggtacgt tccagatcca gcagcatgct
     9061 gtccgccgga aaggtgctgc cgaattcctc ttgcaggtga tacgcacggt gcagcaggct
     9121 ctccagcttt tccgcgaact ctttacgctg gctttccggc tcgctcagcg gctcgctcgc
     9181 ggtttccggg ttacgcagac catcacgcgg catcggggtg ctaccgctgt cgccacgttg
     9241 accaacgctc agcgcttgct cccacttctg cagcgcttcc tcaaacagct ccataccctg
     9301 catgtacagg ctttccgcgt tgccatcgct ggtggtcagg ctttcctcca taccacgcgc
     9361 atcccacagg ccgctgcacg ccgcggtcgg gctgctgctg ttaaccgcca tcatgctcgc
     9421 cacgctgtgg ctgctaccgc tgtgcttgct cggttcaatg ctgctgatgc cgctcagggt
     9481 gtcgttgctt ttgctgctcg ggctttgcac acgacggctg ctatagcctt tcttaacgct
     9541 cggcacttta cgcgccagca ggatcggcag cggaacggta cccagttgct cgccacccat
     9601 ttccgggccc acttgcttct tacgacgacg acgacgcttc agctggtgcg ccgccagcgc
     9661 cagcgcaacg gtacccagcg cggtcgcgaa cagcacttta cgcaggcccg gggtcagacg
     9721 cagttggcta aacgcgctct gaccaaaggt ggtgtacagg aaaaccggaa tctccgccac
     9781 ggtcatcgcc agcgcctgga tcatgctggt gccttccgca cgacgaaacg ccatgaattc
     9841 acctgcaggc caccacactg gggtacctac gtaagtactc aattgaggtc gaaaggcccg
     9901 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     9961 ggcctccgga ggaccttcgg gcgcccgccc cg
//
