LOCUS       sequence-379968-        4335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BR1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Liang GT, Lai C, Yue Z, Zhang H, Li D, Chen Z, Lu X, Tao L, Subach 
            FV, Piatkevich KD
  TITLE     Enhanced small green fluorescent proteins as a multisensing platform
            for biosensor development.
  JOURNAL   Front Bioeng Biotechnol. 2022 Oct 17;10:1039317. doi: 
            10.3389/fbioe.2022.1039317. eCollection 2022.
  PUBMED    36324888
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2022-10-17"; volume: "10:1039317. doi"; 
            pages: " 10.3389/fbioe.2022.1039317. eCollection 2022"
COMMENT     Sequence Label: pBAD-HisD-BR1
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..349
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     272..291
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             392..409
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(800..817)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      970..1056
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1148..1175
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1194..1285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1286..1354,1355..2146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1504..1523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2317..2905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2806..2825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3059..3076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3091..3231
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3157..3176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3317..3339
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(3495..4335,1..38))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
       61 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
      121 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
      181 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
      241 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
      301 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
      361 gggctaacag gaggaattaa ccatggggag ccaccatcac catcaccatg gcagatctat
      421 gatcgagaag aactacgtga tcaccgaccc cagactgccc gacaacccca tcatcttcgc
      481 cagcgacggc ttcctggagc tgaccggcta cagcagagag gagatcctgg gcagaaacgc
      541 cagattcctg cagggccccg agaccgacca ggccaccgtg cagaagatca gagacgccat
      601 cagagaccag agagagacca ccgtgcagct gatcaactac accaagagcg gcagaaagtt
      661 ctggaacctg ctgcacctgc agcccgtgag agaccagaag ggcgagctgc agtacttcat
      721 cggcgtgcag ctggacggca gcgacagagt gtaagaattc gaagcttggc tgttttggtg
      781 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
      841 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
      901 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      961 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1021 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     1081 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
     1141 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
     1201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1381 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1441 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1501 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     1561 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1681 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1861 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2461 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3001 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3061 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     3121 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     3181 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     3241 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3301 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3361 cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt
     3421 caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc
     3481 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     3541 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     3601 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     3661 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     3721 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     3781 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     3841 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     3901 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     3961 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     4021 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     4081 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     4141 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     4201 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     4261 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     4321 tgggcattaa acgag
//
