LOCUS       sequence-380002-       10289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TadCBEa in mammalian cells with BlastR selection, Tol2 
            integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, 
            Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn
            MJ, Liu DR
  TITLE     Evolution of an adenine base editor into a small, efficient cytosine
            base editor with low off-target activity.
  JOURNAL   Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 
            10.1038/s41587-022-01533-6.
  PUBMED    36357719
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 
            10.1038/s41587-022-01533-6."
COMMENT     Sequence Label: p2T-cmv-SpCas9-TadCBEa-BlastR
FEATURES             Location/Qualifiers
     source          1..10289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..123
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(721..744,745..747,748..4821)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             721..4821
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4852..5100
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5131..5379
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5422..5442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5451..5468
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5491..5508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5497..5721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5792..5808)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5792..5808)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5805..5827)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5816..5832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5840..5846,5847..5864,5865..5870))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5885..5906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6023..6144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6034..6053)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6088..6107
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6302..6700)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6719..6766)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6814..7143)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6828..6963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6882..6901)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7128..7148
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7267..7454,7455..7466)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7527..8115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7607..7626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8286..9077,9078..9146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8909..8928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9147..9251)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9330..9349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9541..9552,9553..9690)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9699..10078
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10079..10282
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10233..10253
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag agatccgcgg ccgctaatac gactcactat agggagagcc
       61 gccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa gaagcggaaa
      121 gtcagttctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc
      181 aagagggcac gggatgaggg ggcgggtcct gtgggagccg tgctggtgct gaacaataga
      241 gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa
      301 attatggccc tgagacaggg cggcctggtc atgcagaact acagactgtt tgacgccacc
      361 ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcaa ctctaggatc
      421 ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg ccgcaggctc cctgatgaac
      481 gtgctgaact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
      541 gaatgtgccg ccctgctgtg cgatttctat cggatgccta gacaggtgtt caattctcag
      601 aagaaggccc agagctccat caactctggc ggatctagcg gaggatcctc tggcagcgag
      661 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
      721 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
      781 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
      841 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
      901 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
      961 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     1021 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     1081 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     1141 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     1201 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     1261 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     1321 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     1381 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     1441 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     1501 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     1561 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     1621 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     1681 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     1741 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     1801 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     1861 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     1921 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     1981 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     2041 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     2101 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     2161 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     2221 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     2281 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     2341 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     2401 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     2461 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     2521 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     2581 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     2641 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     2701 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     2761 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     2821 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     2881 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     2941 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     3001 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     3061 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     3121 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     3181 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     3241 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     3301 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     3361 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     3421 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     3481 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     3541 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     3601 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     3661 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     3721 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     3781 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     3841 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     3901 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     3961 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     4021 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     4081 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     4141 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     4201 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     4261 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     4321 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     4381 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     4441 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     4501 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     4561 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     4621 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     4681 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     4741 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     4801 ctgtctcagc tgggaggtga cagcggcggg agcggcggga gcggggggag cactaatctg
     4861 agcgacatca ttgagaagga gactgggaaa cagctggtca ttcaggagtc catcctgatg
     4921 ctgcctgagg aggtggagga agtgatcggc aacaagccag agtctgacat cctggtgcac
     4981 accgcctacg acgagtccac agatgagaat gtgatgctgc tgacctctga cgcccccgag
     5041 tataagcctt gggccctggt catccaggat tctaacggcg agaataagat caagatgctg
     5101 agcggaggat ccggaggatc tggaggcagc accaacctgt ctgacatcat cgagaaggag
     5161 acaggcaagc agctggtcat ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa
     5221 gtgatcggaa acaagcctga gagcgatatc ctggtccata ccgcctacga cgagagtacc
     5281 gacgaaaatg tgatgctgct gacatccgac gccccagagt ataagccctg ggctctggtc
     5341 atccaggatt ccaacggaga gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc
     5401 gccgacggca gcgaattcga gcccaagaag aagaggaaag tctaaccggt catcatcacc
     5461 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     5521 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5581 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5641 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5701 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
     5761 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5821 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
     5881 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5941 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6001 gtattgggcg ctacagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6061 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6121 attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg
     6181 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     6241 ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg
     6301 cttagccctc ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct
     6361 gtccatcact gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc
     6421 ggcaccgtcc gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag
     6481 tcaggttgcc agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc
     6541 ccccagtaaa atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg
     6601 cgctggcgac gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt
     6661 caatgagggt ggattcttct tgagacaaag gcttggccat ggtttagttc ctcaccttgt
     6721 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga
     6781 tccgaaaatg gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa
     6841 gcctcctcac tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat
     6901 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
     6961 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
     7021 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
     7081 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
     7141 cacattccac agaattcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7261 acatgtaagt gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt
     7321 aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta
     7381 attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa
     7441 aattactcaa gtactttaca cctctgcatg tgagcaaaag gccagcaaaa ggccaggaac
     7501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac
     8161 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8221 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8281 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8341 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8401 ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca
     8461 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8521 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8581 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8641 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8701 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8761 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8821 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8881 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8941 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9001 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9061 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9121 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9181 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9241 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     9301 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9361 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9421 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9481 tttgcgctgc ttcgcgatgt acgggccaga tatacgcggg taccggcata tggttcttga
     9541 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
     9601 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
     9661 agtactccat taaaattgta cttgagtatt aacgcgttga cattgattat tgactagtta
     9721 ttaatagtaa tcaattacgg ggtcattagt ttatagccca tatatggagt tccgcgttac
     9781 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     9841 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     9901 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     9961 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    10021 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    10081 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
    10141 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    10201 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
    10261 ggaggtctat ataagcagag ctggtttag
//
