LOCUS       sequence-380016-        8405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid encoding myc-tagged OGA(GS-544)C181_NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ge Y, Lu H, Yang B, Woo CM
  TITLE     Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of 
            O-GlcNAc in Live Cells.
  JOURNAL   ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894.
  PUBMED    36598936
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894."
COMMENT     Sequence Label: pcDNA3.1(+)-myc-OGA(GS-544)C181_NES
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        297..676
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        677..880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     831..851
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     925..944
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        925..943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             981..1010
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4058..4075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4064..4288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4334..4762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4421..4440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4631..4652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4771..4791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4776..5105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4956..5091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5018..5037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5172..5966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5226..5245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5836..5855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6140..6261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6177..6196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6231..6250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6310..6326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6323..6345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6334..6350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6358..6364,6365..6382,6383..6388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6403..6424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6541..6558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6712..7300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6792..6811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7471..8262,8263..8331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8094..8113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8332..8405,1..31))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
       61 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      121 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      181 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      241 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      301 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      361 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      421 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      481 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      541 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      601 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      661 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      721 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      781 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      841 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      901 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcga
      961 gcgtttaagc ttgagcaatg gagcagaagc tgatcagcga ggaggatctg gcgatcgcaa
     1021 tggtgcagaa ggagagtcaa gcgacgttgg aggagcggga gagcgagctc agctccaacc
     1081 ctgccgcctc tgcgggggca tcgctggagc cgccggcagc tccggcaccc ggagaagaca
     1141 accccgccgg ggctggggga gcggcggtgg ccggggccgc aggaggggct cggcggttcc
     1201 tctgcggtgt ggtggaagga ttttatggaa gaccttgggt tatggaacag agaaaagaac
     1261 tctttagaag gctccagaaa tgggaattaa atacatactt gtatgcccca aaagatgact
     1321 acaaacatag gatgttttgg cgagagatgt attcagtgga ggaagctgag caacttatga
     1381 ctctcatctc tgctgcacga gaatatgaga tagagttcat ctatgcgatc tcacctggat
     1441 tggatatcac tttttctaac cccaaggaag tatccacatt gaaacgtaaa ttggaccagg
     1501 tttctcagtt tgggtgcaga tcatttgctt tgctttttga tgatatagac cataatatgt
     1561 gccttgccga gggtacccga atcttcgatc cggtcactgg tacaacgcat cgcatcgagg
     1621 atgttgtcga tgggcgcaag cctattcatg tcgtggctgc tgccaaggac ggaacgctgc
     1681 tcgcgcggcc cgtggtgtcc tggttcgacc agggaacgcg ggatgtgatc gggttgcgga
     1741 tcgccggtgg cgccatcgtg tgggcgacac ccgatcacaa ggtgctgaca gagtacggct
     1801 ggcgtgccgc cggggaactc cgcaagggag acagggtggc cggaccgggt ggttctggta
     1861 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     1921 cccccatact ctattccgag tatgatccta ccagtccctt cagtgaagct tcgatgatgg
     1981 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     2041 tgccaggctt tgtggatttg accctccatg atcaggccca ccttctagaa cgtgcctggc
     2101 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccaggg aagctactgt
     2161 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     2221 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     2281 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     2341 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agccctggac aagatcacgg
     2401 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     2461 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggaatggagc
     2521 atctgtacag catgaagtac aagaacgtgg tgcccctcta tgacctgctg ctggagatgc
     2581 tggacgccca ccgcctacat gcgggtggtt ctggtgctag ccgcgtgcag gcgttcgcgg
     2641 atgccctgga tgacaaattc ctgcacgaca tgctggcgga aggactccgc tattccgtga
     2701 tccgagaagt gctgccaacg cggcgggcac gaacgttcga cctcgaggtc gaggaactgc
     2761 acaccctcgt cgccgaaggg gttgtcgtgc acaactgcgc agcagacaaa gaggtattca
     2821 gttcttttgc tcatgcccaa gtctccatca caaatgaaat ctatcagtac ctaggagagc
     2881 cagaaacttt cctcttctgt cccacagaat actgtggcac tttctgttat ccaaatgtgt
     2941 ctcagtctcc atatttaagg actgtgggtg aaaagcttct acctggaatt gaagtgcttt
     3001 ggacaggtcc caaagttgtt tctaaagaaa ttccagtaga gtccatcgaa gaggtttcta
     3061 agattattaa gagagctcca gtaatctggg ataacattca tgctaatgat tatgatcaga
     3121 agagactgtt tctgggcccg tacaaaggaa gatccacaga actcatccca cggttaaaag
     3181 gagtcctcac taatccaaat tgtgaatttg aagccaacta cgttgctatc cacacccttg
     3241 ccacctggta caaatcaaac atgaatggag tgagaaaaga tgtagtgatg actgacagtg
     3301 aagatagtac tgtgtccatc cagataaaat tagaaaatga aggcagtgat gaagatattg
     3361 aaactgatgt actctatagt ccacagatgg ctctaaagct agcattaaca gaatggttgc
     3421 aagagtttgg tgtgcctcat caatacagca gtaggggaag cggctccgga agtggatctg
     3481 gaagtggatc cggaagcgga gaaaagcctt tgtacactgc ggaaccagtg accctggagg
     3541 atttgcagtt acttgctgat ctattctacc ttccttacga gcatggaccc aaaggagcac
     3601 agatgttacg ggaatttcaa tggcttcgag caaatagtag tgttgtcagt gtcaattgca
     3661 aaggaaaaga ctctgaaaaa attgaagaat ggcggtcacg agcagccaag tttgaagaga
     3721 tgtgtggact agtgatggga atgttcactc ggctctccaa ttgtgccaac aggacaattc
     3781 tttatgacat gtactcctat gtttgggata tcaagagtat aatgtctatg gtgaagtctt
     3841 ttgtacagtg gttagggtgt cgtagtcatt cttcagcaca attcttaatt ggagaccaag
     3901 aaccctgggc ctttagaggt ggtctagcag gagagttcca gcgtttgctg ccaattgatg
     3961 gggcaaatga tctctttttt cagccacctc tggccctgaa gctggccggc ctggacatca
     4021 actaagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4081 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4141 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4201 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4261 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4321 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4381 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4681 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4741 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5041 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5101 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5161 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5221 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5281 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5341 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5401 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5461 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5521 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5581 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5641 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5701 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5761 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5821 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5881 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5941 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6001 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6061 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6121 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6241 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6361 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6481 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8041 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8101 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8161 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8221 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8281 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8341 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8401 atgta
//
