LOCUS       sequence-380018-        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid encoding myc-tagged OGA(GS-544)C181.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Ge Y, Lu H, Yang B, Woo CM
  TITLE     Small Molecule-Activated O-GlcNAcase for Spatiotemporal Removal of 
            O-GlcNAc in Live Cells.
  JOURNAL   ACS Chem Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894.
  PUBMED    36598936
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2023 Jan 4. doi: 10.1021/acschembio.2c00894."
COMMENT     Sequence Label: pcDNA3.1 (+) -myc-OGA(GS-544)C181
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..796
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        777..795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             833..862
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3877..3894)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3883..4107
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4153..4581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4240..4259)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4450..4471
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4590..4610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4595..4924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4775..4910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4837..4856
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4991..5785
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5045..5064)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5655..5674
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5959..6080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5996..6015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6050..6069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6129..6145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6129..6145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6142..6164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6153..6169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6177..6183,6184..6201,6202..6207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6222..6243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6360..6377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6531..7119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6611..6630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7290..8081,8082..8150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7913..7932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8151..8255)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8330..8349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
       61 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      781 acgactcact atagggagac ccaagctggc gagcgtttaa gcttgagcaa tggagcagaa
      841 gctgatcagc gaggaggatc tggcgatcgc aatggtgcag aaggagagtc aagcgacgtt
      901 ggaggagcgg gagagcgagc tcagctccaa ccctgccgcc tctgcggggg catcgctgga
      961 gccgccggca gctccggcac ccggagaaga caaccccgcc ggggctgggg gagcggcggt
     1021 ggccggggcc gcaggagggg ctcggcggtt cctctgcggt gtggtggaag gattttatgg
     1081 aagaccttgg gttatggaac agagaaaaga actctttaga aggctccaga aatgggaatt
     1141 aaatacatac ttgtatgccc caaaagatga ctacaaacat aggatgtttt ggcgagagat
     1201 gtattcagtg gaggaagctg agcaacttat gactctcatc tctgctgcac gagaatatga
     1261 gatagagttc atctatgcga tctcacctgg attggatatc actttttcta accccaagga
     1321 agtatccaca ttgaaacgta aattggacca ggtttctcag tttgggtgca gatcatttgc
     1381 tttgcttttt gatgatatag accataatat gtgccttgcc gagggtaccc gaatcttcga
     1441 tccggtcact ggtacaacgc atcgcatcga ggatgttgtc gatgggcgca agcctattca
     1501 tgtcgtggct gctgccaagg acggaacgct gctcgcgcgg cccgtggtgt cctggttcga
     1561 ccagggaacg cgggatgtga tcgggttgcg gatcgccggt ggcgccatcg tgtgggcgac
     1621 acccgatcac aaggtgctga cagagtacgg ctggcgtgcc gccggggaac tccgcaaggg
     1681 agacagggtg gccggaccgg gtggttctgg taacagcctg gccttgtccc tgacggccga
     1741 ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc
     1801 taccagtccc ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga
     1861 gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca
     1921 tgatcaggcc caccttctag aacgtgcctg gctagagatc ctgatgattg gtctcgtctg
     1981 gcgctccatg gagcacccag ggaagctact gtttgctcct aacttgctct tggacaggaa
     2041 ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc
     2101 tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt
     2161 gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga
     2221 ccatatccac cgagccctgg acaagatcac ggacactttg atccacctga tggccaaggc
     2281 aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca
     2341 catcaggcac atgagtaaca aaggaatgga gcatctgtac agcatgaagt acaagaacgt
     2401 ggtgcccctc tatgacctgc tgctggagat gctggacgcc caccgcctac atgcgggtgg
     2461 ttctggtgct agccgcgtgc aggcgttcgc ggatgccctg gatgacaaat tcctgcacga
     2521 catgctggcg gaaggactcc gctattccgt gatccgagaa gtgctgccaa cgcggcgggc
     2581 acgaacgttc gacctcgagg tcgaggaact gcacaccctc gtcgccgaag gggttgtcgt
     2641 gcacaactgc gcagcagaca aagaggtatt cagttctttt gctcatgccc aagtctccat
     2701 cacaaatgaa atctatcagt acctaggaga gccagaaact ttcctcttct gtcccacaga
     2761 atactgtggc actttctgtt atccaaatgt gtctcagtct ccatatttaa ggactgtggg
     2821 tgaaaagctt ctacctggaa ttgaagtgct ttggacaggt cccaaagttg tttctaaaga
     2881 aattccagta gagtccatcg aagaggtttc taagattatt aagagagctc cagtaatctg
     2941 ggataacatt catgctaatg attatgatca gaagagactg tttctgggcc cgtacaaagg
     3001 aagatccaca gaactcatcc cacggttaaa aggagtcctc actaatccaa attgtgaatt
     3061 tgaagccaac tacgttgcta tccacaccct tgccacctgg tacaaatcaa acatgaatgg
     3121 agtgagaaaa gatgtagtga tgactgacag tgaagatagt actgtgtcca tccagataaa
     3181 attagaaaat gaaggcagtg atgaagatat tgaaactgat gtactctata gtccacagat
     3241 ggctctaaag ctagcattaa cagaatggtt gcaagagttt ggtgtgcctc atcaatacag
     3301 cagtagggga agcggctccg gaagtggatc tggaagtgga tccggaagcg gagaaaagcc
     3361 tttgtacact gcggaaccag tgaccctgga ggatttgcag ttacttgctg atctattcta
     3421 ccttccttac gagcatggac ccaaaggagc acagatgtta cgggaatttc aatggcttcg
     3481 agcaaatagt agtgttgtca gtgtcaattg caaaggaaaa gactctgaaa aaattgaaga
     3541 atggcggtca cgagcagcca agtttgaaga gatgtgtgga ctagtgatgg gaatgttcac
     3601 tcggctctcc aattgtgcca acaggacaat tctttatgac atgtactcct atgtttggga
     3661 tatcaagagt ataatgtcta tggtgaagtc ttttgtacag tggttagggt gtcgtagtca
     3721 ttcttcagca caattcttaa ttggagacca agaaccctgg gcctttagag gtggtctagc
     3781 aggagagttc cagcgtttgc tgccaattga tggggcaaat gatctctttt ttcagccacc
     3841 ttaagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     3901 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3961 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4021 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4081 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4141 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4201 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4261 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4321 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4381 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4441 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4501 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4561 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4861 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4921 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4981 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5041 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5101 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5161 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5221 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5281 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5341 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5401 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5461 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5521 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5581 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5641 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5701 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5761 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5821 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5881 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5941 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6001 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6061 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8281 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8341 ctgatgccgc atagttaagc cagtatctgc tc
//
