LOCUS       sequence-380038-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of the hydrogen 
            peroxide sensor Hyper7. Parton lab clone KQV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: pME-p3E-HyPer7
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(16..102)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    197..296
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1743..1838)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1875..1894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1876..1894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1899..1915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2017..2036)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2028..2837
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2095..2114)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2984..3572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3473..3492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3726..3743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3902..3929)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
       61 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      121 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtcttaagct cgggcccaaa taatgatttt attttgactg atagtgacct gttcgttgca
      241 acacattgat gagcaatgct tttttataat gccaactttg tacaaaaaag caggctgcca
      301 ccatgcattt ggcgaatgaa gaacaaaatg agctggaggg tgcgtttaag ttgggcctca
      361 tcttcacggt ggcaccctac cttcttccta aacttatcgt atctctgcgg aggaccgcgc
      421 ctaagatgcc cttgatgctc gaggagaact acacacatac gctcacagag tcactcaaga
      481 ggggagacgt cgacgcaatc attgtcgcag aaccgttcca agagccgggt atcgtgacgg
      541 agcctctcta cgatgagccg ttcttcgtga tagtcccgaa gggtcacagt ttcgaggagc
      601 ttgatgctgt ttcccctcgg atgcttggtg aagagcaagt tctcctcctt acagagggga
      661 attgtatgag ggatcaagta ctgagcagcg cgggctttaa tagcaacaat gtatatataa
      721 tggcagacaa acagaaaaat ggcataaagg caaattttaa gattcgtcat aatgtagaag
      781 acggtagcgt tcaactcgcc gatcactacc agcaaaacac tccaatcggt gacgggcctg
      841 tactcctccc ggataaccat tacctctcat tccagagtgt actcagtaaa gatcctaacg
      901 agaaaagaga tcatatggtc ttgttggagt ttgttacagc agcggggatc accctgggca
      961 tggatgagct ttataacgta gatggcggtt ccggaggaac gggttctaag ggtgaagaat
     1021 tgtttaccgg agtagttcct atcttggtcg aactggatgg ggacgttaac ggccataaat
     1081 tttcagtttc tggggaaggc gagggagatg caacttatgg caagctgacc ctcaaactca
     1141 tctgcacgac gggaaagctc ccagttcctt ggcctacgct ggtgaccacg ctgtcttatg
     1201 gcttgaagtg ttttgcgcgt tacccagacc acatgaagca gcatgacttt tttaagtctg
     1261 ccatgcccga ggggtacgtt caggagagaa caattttctt caaagacgac gggaactaca
     1321 aaacaagagc cgaagtgaaa tttgaagggg ataccttggt gaatcgcatc gagctgaagg
     1381 gaatcggatt caaagaagat ggcaacatcc ttgggcacaa actcgagtat aacggcccga
     1441 gttgtagtga gcttgcggcc aagcagcgta tccagggtct tacaaataca ttgcaaggat
     1501 cttctatcaa cacaattcgg cacatggtcg ccagtgggct cgcaatctct gtacttccgg
     1561 ccaccgcgtt gacggagaac gatcatatgc tgttctccat cattcccttt gagggaacgc
     1621 cgccatccag aagggtggtt ctcgcttatc gaaggaactt tgttcggcca aaggccttgt
     1681 ctgctatgaa ggccgctatt atgcaaagcc agttgcatgg ggtgagcttt atatgcgatt
     1741 gacaacttta ttatacaaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     1801 cgaacaggtc actatcagtc aaaataaaat cattatttgg agctccatgg tagcgttaac
     1861 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1921 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1981 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     2041 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     2101 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     2161 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     2221 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     2281 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     2341 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     2401 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     2461 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     2521 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2581 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2641 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2701 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2761 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2821 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     2881 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     2941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3781 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3841 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc
     3901 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg
     3961 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaa
//
