LOCUS       sequence-380039-        3184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of codon optimised 
            zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab 
            clone KRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: p3E-DrCav1
FEATURES             Location/Qualifiers
     source          1..3184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(166..193)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(285..371)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     420..442
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     434..451
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     435..451
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(522..646)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1191..1286)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1323..1342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1324..1342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1347..1363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1347..1363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1465..1484)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1476..2285
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1543..1562)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2432..3020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2921..2940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3174..3184,1..7)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      121 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
      181 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      241 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      301 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      361 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      421 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccctgca gctctagagc
      481 tcgaattcta caggtcacta ataccatcta agtagttggt tcatagtgac tgcatatgtt
      541 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      601 ttatatcatt ttacgtttct cgttcaactt tcttgtacaa agtggtgact agcggataca
      661 aggacgggac acctgaagag gaatacgctc actcgccgtt tatcaggaaa caggggaaca
      721 tttacaaacc aaacaataaa gaaatggata acgacagcat caacgaaaag acacttcagg
      781 atgtccacac caaggagatt gacctggtca accgagaccc aaagcattta aatgacgatg
      841 tggtgaaggt ggactttgag gacgtgatcg ccgagcctgc cggcacctac agcttcgacg
      901 gcgtgtggaa ggcgagcttc accaccttca cagtaaccaa atattggtgc tacaggctgc
      961 tgacagcgct ggtgggcatc ccactcgccc tggtatgggg catcttcttc gccatcctct
     1021 ccttcatcca catctgggcc gtggtgcctt gcgtgaagag ctacctaatc gagatccact
     1081 gcatcagtcg agtttactcc atctgtgtgc acaccttctg cgacccactc tttgaggcca
     1141 tggggaaatg ctttagtaac gtccgggtca ctgctactaa ggtggtgtaa caactttatt
     1201 atacaaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1261 tatcagtcaa aataaaatca ttatttggag ctccatggta gcgttaacgc ggccgcgata
     1321 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     1381 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     1441 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     1501 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     1561 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     1621 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     1681 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1741 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     1801 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1861 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     1921 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     1981 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     2041 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     2101 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     2161 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     2221 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     2281 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     2341 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     2401 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     2581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3181 cagt
//
