LOCUS       sequence-380041-        3886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of codon optimised 
            zebrafish cavin1a with fusion tag at the N-terminus. Parton lab 
            clone KXM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: p3E-DrCavin1a
FEATURES             Location/Qualifiers
     source          1..3886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(44..130)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     179..201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     193..210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     194..210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(281..405)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1652..1747)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1784..1803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1785..1803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1808..1824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1808..1824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1926..1945)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1937..2746
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2004..2023)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2893..3481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3382..3401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3635..3652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3811..3838)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
       61 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
      121 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
      181 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct
      241 cgaattctac aggtcactaa taccatctaa gtagttggtt catagtgact gcatatgttg
      301 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      361 tatatcattt tacgtttctc gttcaacttt cttgtacaaa gtggtggctg atacagactt
      421 taagctggaa agggctattt tcgctgaggt aagcgatgat gacgaagagg ttgccctggt
      481 cacatctgcc gcgaaaccag ccaccagtct taaagaattt aagtcacggc gaggggatga
      541 tgaagatgac gacgatgata gcgagaaaga agttgatttg gtattgggac cggggctgga
      601 tgggggggac gcagagaagt ctgacgccac tggcatactt gtgttggcct tgctggacaa
      661 gatcattggt gtagtagaca aaatacaaca aacacaaact tcattggaag caaaacagga
      721 agtgatggaa aagaatatga gttcaataca gacagaattg gcaaaacttg ccaagtcaca
      781 cgtcggtacg gcgggtaccg tgaataagct cttggacaaa gttaggaaag ttaatgtcaa
      841 tgtcaagagt gtgagggccg acttggagaa gcaggtgggg cagatcaaaa aattggagaa
      901 caatgaacaa gaacttctga aacgcaaaaa cataaaagtg ttgataatcc ccgaaaaagg
      961 aaagcctgta aaggtgtcaa agaaacctgc cggtgaagag ggagagcagg ttctgtccga
     1021 gggtggtgag gaagagcatg cttctgaggg agaggaggtc gaggtagaag agaccattga
     1081 agaaagtaga gctgaaagaa ttaaacgatc cggtctccag aaggtcgatg aattgaaaaa
     1141 agcattctcc aaggagcaga tgcaaaagac caggcagaaa actaaggaaa atctcgagaa
     1201 gacaaggatg cggactaaag agaatttgga gaagacacgg caacgcacgc gcgagaatct
     1261 cgaaaagact aagaagtccc ttggtaaaaa aatgggcaaa ttcggcacca agcttacccc
     1321 gaacactgag agaagggaga agataaggtc atcccgtgaa aaaatgaaaa aaagccttaa
     1381 cccggatcac accgtgtatg cccgctcaaa gtctacgact tatcgcgtac caccattcac
     1441 attccatgtc aagaaaattc gtggtggtga agcagagccg acaccggagc cagaagagga
     1501 agatgaagag cagaaggaag aagaggaaga aggcgacctg gttgaggagg ccgaactcgt
     1561 tagccttgat ggaccagaaa tggaggcggc tgatcgatct cgcctcgcct ttcaggaaac
     1621 cgttcgccct agagagaccg ctggcctgta gcaactttat tatacaaagt tggcattata
     1681 aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1741 attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta
     1801 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     1861 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     1921 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1981 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2041 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2101 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2161 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2221 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2281 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2341 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2401 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2461 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2521 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2581 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2641 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2701 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2761 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     2821 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     2881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3001 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3181 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3241 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3301 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3361 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3421 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3481 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3541 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3601 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3661 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     3721 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     3781 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     3841 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccacc
//
