LOCUS       sequence-380042-        4003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of codon optimised 
            zebrafish cavin1b with fusion tag at the N-terminus. Parton lab 
            clone KXJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: p3E-DrCavin1b
FEATURES             Location/Qualifiers
     source          1..4003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(74..98)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1462..1557)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1594..1613)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1595..1613)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1618..1634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1618..1634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1736..1755)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1747..2556
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1814..1833)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2703..3291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3192..3211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3445..3462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3621..3648)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3740..3826)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3875..3897
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3889..3906
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3890..3906
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
       61 ttttacgttt ctcgttcaac tttcttgtac aaagtggtgg aggtcattga agatagtcgt
      121 ttgcatcttg aagtacccga actgcggaag tcattgacgg agataagcga ggatgaaatc
      181 aatctcccag tctctgacga tgaggacgag gctacagctg aaaataaagc caaagatgca
      241 gcggaaaagg aagaagctgg cgagggcggc gagatgaacg gggtaatggt cctcacattg
      301 cttgataaaa ttatcggtgc agtggatcag attcaacaaa cacaggctgg ccttgaagct
      361 cgtcagcgag acatggagcg atccgtaacc ggcatccagg gagagctgac gaagcttagt
      421 aagtctcata caactactgc gaacactgta aacaaaatgt tggagaaggt gcgtaaagta
      481 agtgtgaacg tgaaatccgt ccgggggaac ctggaaaaac aagcaggcca aattaagcgc
      541 ctggagagta atgagaatga actcttgaaa cgacgcaatt ttaaggtgat gatataccag
      601 gacgaggtca aggtaggatc aaaggtgaat gttgcaaaga gcatgaagta ccctgagcct
      661 gtagagggag aggccgaagg agagttgaaa gcggcggcgg aggagggagg agaggacaaa
      721 gaaaaggtga atctggacct gtccagtgat gaagaggaag tcgagataga ggagacaatc
      781 gaggaatccc gagcggaacg aatcaaacga tctagcctgc agagagtaca gaacatcaag
      841 actgttttct ctaaagggaa catggagaag acaaagcaga aaacgaagga aaacttggag
      901 aagacaaaac agaagacgaa ggaaaacctc gaaaaaacgc ggatgcgcac caaggagaac
      961 cttgagaaga cccgtcagaa aaccaaggaa aaccttgaga aaacgaagca aaaaacgcgg
     1021 gaaaatctgg aaaagacaag acacaatatt gaaaagaaga tgggtaagct gggcactagg
     1081 atgtcagtca atccagagcg taaggagaag atgaaaacaa gccagaaaaa ggtcatgaag
     1141 agtttcactc ccgatcatac catttacgcg cgctccaaga ctgcggtgta caaggtccct
     1201 cccttcacct ttcacgtcaa gaagatccgt gagggtgagg tcgagattca ggggacggaa
     1261 atggtggaag tggtagggga caccgaggag gttgaaaacg cggtaatgga gggggaggag
     1321 gatgctgaga tggaaatgga ggcgggcgag atggtcgagg aagacgaaga cgagactgag
     1381 gactcacctg aaatgcgcgc acttcttgag aagggagatg aattggtatt ggtagaggac
     1441 catgacagag attctgacta gcaactttat tatacaaagt tggcattata aaaaagcatt
     1501 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga
     1561 gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta ttacatggtc
     1621 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1681 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1741 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1801 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1861 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1921 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1981 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     2041 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     2101 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     2161 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     2221 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2281 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2341 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2401 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2461 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2521 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2581 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2641 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3601 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3661 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3721 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3781 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3841 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3901 gccagtctta agctcgggcc ctgcagctct agagctcgaa ttctacaggt cactaatacc
     3961 atctaagtag ttggttcata gtgactgcat atgttgtgtt tta
//
