LOCUS       sequence-38005-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA
  TITLE     A new DAF-16 isoform regulates longevity.
  JOURNAL   Nature. 2010 Jul 22. 466(7305):498-502.
  PUBMED    20613724
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 22. 466(7305):498-502."
COMMENT     Sequence Label: L4440-daf-16af
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10..26
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(376..392)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(449..468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(450..468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(492..509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(743..762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccccccctc gaggtcgacg gtatcgataa gcttatacgg gagcaatgag caatgtggac
       61 agctcggcgg agcatcttca aacgggtcga cagcaatgct tcatactcca gatggaagca
      121 attctcatca gacatcgttt ccttcggatt tcagaatgtc cgaatcgcca gacgataccg
      181 tatcgggaaa aaagacaacg accagacgga acgcttgggg aaatatgtca tatgctgaac
      241 ttatcactac agccattatg gctagtccag agaaacggtt aactcttgca caagtttacg
      301 aatggatggt ccagaatgtt ccatacttca gggataaggg agattcgaac agttcagcta
      361 gccatggaac cggtggatcc actagttcta gagcggccgc caccgcggtg gagctcgaat
      421 tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc
      481 caggttgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      541 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      781 cgctctcctg ttccgaccct gccgcttacc gg
//
