LOCUS       sequence-380074-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of mCherry fused to human Gata2 with 
            mutation T354M in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: SFFV-mCh-GATA2-T354M
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1950..2405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2087..2108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2299..2318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2532..2554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2546..2563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2547..2563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2573..2592
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2573..2591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2612..2747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2787..2908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2852..2871
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2980..3213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3306..3709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5366..6067)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(5449..5472)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5647..5666)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5916..5934
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(6102..6508)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6542..6659
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6926..6970)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7155..7388
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7881..8006
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8053..8233
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8234..8460)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8488..8508)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8488..8506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8527..8543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8527..8543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8540..8562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8551..8567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8575..8581,8582..8599,8600..8605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8620..8641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     1981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2401 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2461 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     2521 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     2581 ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc tcactacttc
     2641 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     2701 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     2761 ggcgggatag ctagagccag acatgataag atacattgat gagtttggac aaaccacaac
     2821 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2881 aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa
     2941 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     3001 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3061 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3121 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     3181 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     3241 taagatctac agctgccttg taagtcattg gtcttaaagg tacagcttga tatcgaattg
     3301 caaaggacgt cagcttcccc gacaacacca cggaattatc agtgcccagc aacctagccc
     3361 ctgtccagca gcgggcaagg caggcggcga tgagttctgc cgtggcgatc gggaggggga
     3421 aagcgaaagt cccagaaagg agttgacagg tggtggcaat gccccagcca gtgggggttg
     3481 cgtcagcaaa cacagagcac accacgccac gttgacggac aacgggccac aactcctcat
     3541 aaagagacag caaccaggat ttatacaagg aggagaaaac gaaagccgta cgggaagcaa
     3601 tagcatgata cagaggcatt aaagcagcat atccacacag cgtaaaagga gcaacatagt
     3661 taagaatatc agtcaatctt tcacaaattt tgtaatccag aggttgatta acaggaacag
     3721 agcgtaaata acgggaaagt ttcttaacat gtttgtcttg tggcaataca cctgaactag
     3781 taattacata tccctaaaaa tgtaaatgat tgccccacca ttttgtttta ttaacattta
     3841 aatgtatacc caaatcaaga aaaacagaac aaatatggga ataaatggcg gtaagatgct
     3901 cgaattcctg cagcccgggg gatccctagc ccatggcggt caccatgctg gacgggtggg
     3961 ggtggccgaa ggagaggctg gaggaggggt ggatgggcgt cggagtgggc aggatgtgtc
     4021 cggagtggct gaagggcggg aggtggccca caggtgccat gtgtccagcc agggcagctg
     4081 cactgaaggg ggatgacttc tcctgcatgc actttgacag ctcctcgaag cactccgccc
     4141 ctttcttgct cttcttggac ttgttggaca tcttccggtt ccgagtctgg atcccttcct
     4201 tcttcatggt cagtggcctg ttaacattgt gcagcttgta gtagaggcca caggcgttgc
     4261 agacagggtc cccgttggcg tttcggcgcc ataaggtggt ggttgtcatc tgacaatttg
     4321 cacaacaggt gccggctctt ctggcggccg acagtcttcg cttgggcttg atgagtggtc
     4381 ggttctgccc attcatcttg tggtagaggc cacaggcatt gcacaggtag tggccggtgc
     4441 cgtcccgccg ccagagaggg gtggctgtgg ccccacagtt gacacactcc cggccttctg
     4501 aacaggaacg agccttgctg cgctgcttag gggtgaagct ggaggccggt ccccccagga
     4561 agcctccggg gtggaagagt ccgctgctgt agtcgtgggc agccgccggc acataggagg
     4621 ggtaggtggg gatggggtgg tgtgtagcag gctgggtgcc catagtagct aggcctgggc
     4681 gcaggggact gccactttcc atcttcatgc tctccgtcag tgacacctgg tacttgacgc
     4741 cgtccttgtc ctctcctcgg gctgcactac cccccgcgga agatgaggct ggagacgcag
     4801 cccccgtggt gctagggtca ggagacactt ctttgggtgg cgtgggtggg aagccgaaaa
     4861 ggtgggagcc agagtgggct gctgtagggg tgagggaggc cactgagctc ccgctgcctc
     4921 ccccgctccc acccccagcc cctgggtaca cagagagtgg gcctccaggg cctccagcag
     4981 ctgaggggtg cagtggcgtc ttggagaagg ggctcacggt ccaggggttg tggtggtggg
     5041 ccgcagcggc agagagggct gctttgcccc cgtccagcca gggcaaaccc gggctgtgca
     5101 acaagtgtgg gcggcacatc tggcctccgg tcaggcgggc gtgcgcgggg ctgtaggaga
     5161 cgcgcgcccg cgcgtgagcg gggttggcat agtaggggtt gccctgcgag tcgaggtgat
     5221 tgaagaagac gtccacctcg tctggaggca gcagctgcgc gggttccatg tagttgtgcg
     5281 ccaggcccgg gtggtgtgag tcggggtgct gcgcattcag cacggccggg tgcgccatcc
     5341 agcgcggctg ctcgggcgcc acctccttgt acagctcgtc catgccgtac aggaacaggt
     5401 ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg
     5461 tgatgtccag cttggtgtcc acgtagtagt agccgggcag ttgcacgggc ttcttggcca
     5521 tgtagatggt cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctggt
     5581 ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc
     5641 ccatggtctt cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcatcttca
     5701 ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg ggtcacggtc accagaccgc
     5761 cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac agcttcttgt
     5821 aatcggggat gtcggcgggg tgcttcacgt acgccttgga gccgtacatg aactgggggg
     5881 acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttggcggtct
     5941 gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca
     6001 tggagccctc catgcgcacc ttgaagcgca tgaactcttt gatgacctcc tcgcccttgc
     6061 tcaccatgga tccgtcgacg gtatcgataa gcttgattcg accggccgac tcagtctgtc
     6121 ggaggactgg cgcgccgagt gaggggttgt gagctctttt atagagctcg ggaagcagaa
     6181 gcgcgcgaac agaagcgaga agcaggctga ttggttaatt caaataaggc gcagggtcat
     6241 ttcaggtcct tgggggagcc tggaaacatc tgatgggtct taagaaactg ctgagggttg
     6301 ggccatatct ggggaccatc tgttcttggc ctcgggccgg ggccgaaact gcggtgacca
     6361 tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg cagatatcct gtttggccca
     6421 acgttagcta ttttcatgta cccgcccttg atctgaactt ctctattctt ggtttggtat
     6481 ttttccatgc cttgcaaaat ggcgttactg cagccagctc gaggctagtc tcgtgatcga
     6541 taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     6601 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
     6661 ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     6721 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     6781 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     6841 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     6901 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6961 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     7021 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     7081 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     7141 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     7201 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     7261 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     7321 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     7381 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     7441 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     7501 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     7561 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     7621 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     7681 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     7741 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     7801 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     7861 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     7921 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     7981 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     8041 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     8101 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     8161 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     8221 accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg
     8281 gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac
     8341 gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg
     8401 catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca
     8461 ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     8521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8701 atgaatcggc caacgcgcgg ggagaggcg
//
