LOCUS       sequence-38009-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     C-terminal fluorescent fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET MBP mCherry LIC cloning vector (MBP-mCherry)
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(138..158)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(216..239)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
       61 tccttgatga tggccatgtt atcctcctcg cccttgctca cggatccgct gctgccgttg
      121 ctcccactac caatattgga ttggaagtac aggttttcct cgatcccgag gttgttgtta
      181 ttgttattgt tgttgttgtt cgagctcgaa ttagtctgcg cgtctttcag ggcttcatcg
      241 acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg
      301 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat agtggcggca
      361 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgctacggc acccagcggt
      421 ttgtctttat taaccgcttc cagaccttca tcagtcagca gatagttttc gaggaactct
      481 tttgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac gccaacgaac
      541 ggtttggatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg
      601 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta
      661 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaatcagg
      721 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac gtctttaatg
      781 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag
      841 gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct
      901 ttaatccagc gcggggaact cttcccag
//
