LOCUS       sequence-380100-        4456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway entry clone for expression of human NRF2 (NFE2L2) 
            with fusion tag at the N-terminus. Parton lab clone KRS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Wu Y, Lim YW, Stroud DA, Martel N, Hall TE, Lo HP, Ferguson C, Ryan 
            MT, McMahon KA, Parton RG
  TITLE     Caveolae sense oxidative stress through membrane lipid peroxidation 
            and cytosolic release of CAVIN1 to regulate NRF2.
  JOURNAL   Dev Cell. 2023 Mar 13;58(5):376-397.e4. doi: 
            10.1016/j.devcel.2023.02.004. Epub 2023 Feb 28.
  PUBMED    36858041
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.02.004"; journalName: "Dev Cell"; date: 
            "2023-03-13- 13"; volume: "58"; issue: "5"; pages: "376-397.e4"
COMMENT     Sequence Label: p3E-HsNRF2 (NFE2L2)
FEATURES             Location/Qualifiers
     source          1..4456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(26..50)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1867..1962)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1999..2018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2000..2018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2023..2039)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2023..2039)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2141..2160)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2152..2961
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2219..2238)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3108..3696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3597..3616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3850..3867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4026..4053)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4145..4231)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4280..4302
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4294..4311
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4295..4311
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggt gatggacttg
       61 gagctgccgc cgccgggact cccgtcccag caggacatgg atttgattga catactttgg
      121 aggcaagata tagatcttgg agtaagtcga gaagtatttg acttcagtca gcgacggaaa
      181 gagtatgagc tggaaaaaca gaaaaaactt gaaaaggaaa gacaagaaca actccaaaag
      241 gagcaagaga aagccttttt cgctcagtta caactagatg aagagacagg tgaatttctc
      301 ccaattcagc cagcccagca catccagtca gaaaccagtg gatctgccaa ctactcccag
      361 gttgcccaca ttcccaaatc agatgctttg tactttgatg actgcatgca gcttttggcg
      421 cagacattcc cgtttgtaga tgacaatgag gtttcttcgg ctacgtttca gtcacttgtt
      481 cctgatattc ccggtcacat cgagagccca gtcttcattg ctactaatca ggctcagtca
      541 cctgaaactt ctgttgctca ggtagcccct gttgatttag acggtatgca acaggacatt
      601 gagcaagttt gggaggagct attatccatt cctgagttac agtgtcttaa tattgaaaat
      661 gacaagctgg ttgagactac catggttcca agtccagaag ccaaactgac agaagttgac
      721 aattatcatt tttactcatc tataccctca atggaaaaag aagtaggtaa ctgtagtcca
      781 cattttctta atgcttttga ggattccttc agcagcatcc tctccacaga agaccccaac
      841 cagttgacag tgaactcatt aaattcagat gccacagtca acacagattt tggtgatgaa
      901 ttttattctg ctttcatagc tgagcccagt atcagcaaca gcatgccctc acctgctact
      961 ttaagccatt cactctctga acttctaaat gggcccattg atgtttctga tctatcactt
     1021 tgcaaagctt tcaaccaaaa ccaccctgaa agcacagcag aattcaatga ttctgactcc
     1081 ggcatttcac taaacacaag tcccagtgtg gcatcaccag aacactcagt ggaatcttcc
     1141 agctatggag acacactact tggcctcagt gattctgaag tggaagagct agatagtgcc
     1201 cctggaagtg tcaaacagaa tggtcctaaa acaccagtac attcttctgg ggatatggta
     1261 caacccttgt caccatctca ggggcagagc actcacgtgc atgatgccca atgtgagaac
     1321 acaccagaga aagaattgcc tgtaagtcct ggtcatcgga aaaccccatt cacaaaagac
     1381 aaacattcaa gccgcttgga ggctcatctc acaagagatg aacttagggc aaaagctctc
     1441 catatcccat tccctgtaga aaaaatcatt aacctccctg ttgttgactt caacgaaatg
     1501 atgtccaaag agcagttcaa tgaagctcaa cttgcattaa ttcgggatat acgtaggagg
     1561 ggtaagaata aagtggctgc tcagaattgc agaaaaagaa aactggaaaa tatagtagaa
     1621 ctagagcaag atttagatca tttgaaagat gaaaaagaaa aattgctcaa agaaaaagga
     1681 gaaaatgaca aaagccttca cctactgaaa aaacaactca gcaccttata tctcgaagtt
     1741 ttcagcatgc tacgtgatga agatggaaaa ccttattctc ctagtgaata ctccctgcag
     1801 caaacaagag atggcaatgt tttccttgtt cccaaaagta agaagccaga tgttaagaaa
     1861 aactagcaac tttattatac aaagttggca ttataaaaaa gcattgctta tcaatttgtt
     1921 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttggagctcc atggtagcgt
     1981 taacgcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     2041 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     2101 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     2161 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2221 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2281 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2341 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2401 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2461 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2521 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2581 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2641 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2701 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2761 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2821 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2881 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2941 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     3001 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     3061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3841 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3901 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3961 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     4021 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     4081 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     4141 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4201 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     4261 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     4321 gggccctgca gctctagagc tcgaattcta caggtcacta ataccatcta agtagttggt
     4381 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     4441 ctaatttaat atattg
//
