LOCUS       sequence-38011-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of a 
            photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli 
            response to the amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Chin JW, Santoro SW, Martin AB, King DS, Wang L, Schultz PG
  TITLE     Addition of p-azido-L-phenylalanine to the genetic code of 
            Escherichia coli.
  JOURNAL   J Am Chem Soc. 2002 Aug 7;124(31):9026-7.
  PUBMED    12148987
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc."; date: "2002-08-7"; volume: "124(31)"; pages: "9026-7"
COMMENT     Sequence Label: pEVOL-pAzF
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..108
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(164..181)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa
       61 ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc
      121 tctcatccgc caaaacagcc aagctggaga ccgtttaaac tcaatgatga tgatgatgat
      181 ggtcgactta cagacgtttg cgaattggtt ccagaatctt aatcagctct tccgccacgg
      241 cgtttttcag gcgcatcgga tgcagttctt tgttcttaaa caagctttcc agctcctcgt
      301 agctgttgac ggtcaggtcg ccgccaaatt tctccgggcg cttaatcgtc agtgggtatt
      361 ccagaaaata cttggcgatt tccatgatcg ggttaccctc aaccacgcct gccgggcagt
      421 acgctttctt gatcttcgca cggatttctt ccgggctatc gtcaactgca ataaagttac
      481 ccttgctgct gctcatttta ccctcgccat ccaggccggt caggaccgga ttatgaatac
      541 agacaacctt tttcggcagc agttcacgtg ccagcatgtg aattttgcgt tgctccatac
      601 caccgacggc aacatcaaca ccctggtagt gcaacggatt aacttgcata atagggtaaa
      661 taacctcagc aacttttgga ttctcatcc
//
