LOCUS       sequence-380114-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses phage BCP1 NTase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Ho P, Chen Y, Biswas S, Canfield E, Abdolvahabi A, Feldman DE
  TITLE     Bacteriophage antidefense genes that neutralize TIR and STING immune
            responses.
  JOURNAL   Cell Rep. 2023 Mar 22;42(4):112305. doi: 
            10.1016/j.celrep.2023.112305.
  PUBMED    36952342
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-03-22"; volume: "42(4):112305. doi"; pages: " 
            10.1016/j.celrep.2023.112305"
COMMENT     Sequence Label: pET30a BCP1 NTase
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..159
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             178..198
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tnnnnnctag aataattttg nttaacttta agaaggagat atacaatgga tgaaaaaacc
       61 accggatggc gtggggggca tgtggtagaa ggattagcag gagagttaga acaattacgt
      121 gctcgtttag aacaccaccc acagggacag cgcgagcccg gtgggagtgg gtcaggagaa
      181 aacctctact tccaaggtaa aacggtagac ctctctgtca ccggaatgag ctggatggaa
      241 gaacgaacta ttctcttgac gccgtatgga agtaggttgt atgggactga cacggagaat
      301 tccgattggg atttcaaagg agtctgcata ccaccaaagg aatatttcct tggtctcgag
      361 acatttaatg agtataacaa tacggggggt aagaccttta agaacacgaa ggatgatgta
      421 gacattaata tcatacacgt aagcaagttt gttaaagatg ccatgcacgg tgtccccaac
      481 aatattgaag ttctcttcgc acgagagcaa gattacataa ttctgacgga gcttgggcag
      541 gtgttgcgag acaaccgcca tcttttcctg agtaaacaaa ttatcacaaa attcgggggc
      601 tatacccgat cccttactaa taagctgaag aatggcgcag gtaggcaaga attggtcgaa
      661 gaatttggtt acgatacgaa gaacttcatg cagggagtca ggctccaact ttctgccatt
      721 gaaattttgg aaaccggtga ctatagcacc tatcgaccag aaagggactt ccttttgggt
      781 tgccggaatg gagagtatac tagggaacag gccctcgcac ttgtagagag ctacgacgaa
      841 cgcttgcagg ng
//
