LOCUS       sequence-380126-        5861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express a bright monomeric red FP to label eucaryotic cell 
            nuclei. To be used in tissue clearing methods and other fluorescent 
            microscopy methods.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Wang Z, Romanski A, Mehra V, Wang Y, Brannigan M, Campbell BC, 
            Petsko GA, Tsoulfas P, Blackmore MG
  TITLE     Brain-wide analysis of the supraspinal connectome reveals anatomical
            correlates to functional recovery after spinal injury.
  JOURNAL   Elife. 2022 Jul 15;11. pii: 76254. doi: 10.7554/eLife.76254.
  PUBMED    35838234
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-07-15"; volume: "11. pii: 76254. doi"; pages: " 
            10.7554/eLife.76254"
COMMENT     Sequence Label: pAAV2-CAGIG-H2B-V5-mScarlet
FEATURES             Location/Qualifiers
     source          1..5861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(115..219)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(245..700)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(382..403)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     594..613
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   772..901
                     /label=AAV2 ITR
     primer_bind     1791..1813
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             2299..2340
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3076..3664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3129..3149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3547..3558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3707..3828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3718..3737)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3772..3791
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3990..4119
                     /label=AAV2 ITR
     rep_origin      complement(4356..4944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4436..4455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5115..5861,1..45,46..114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5738..5757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
       61 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      241 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
      301 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
      361 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
      421 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
      481 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
      541 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
      601 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
      661 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
      721 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct
      781 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
      841 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
      901 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agacgcgtat agcgctcata
      961 tggaagagta ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1021 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1081 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1141 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1201 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     1261 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     1321 gggggcgggg ggggggggsg cgcgccaggc ggggcggggs ggggsgrggg gsggggsggg
     1381 gsgrggcggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     1441 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggcgcc
     1501 actagtagat cttggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     1561 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     1621 cggcccttct cctccgggct gtaattaggc ttggtttaat gacggcttgt ttcttttctg
     1681 tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg
     1741 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1801 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1861 cctacagctc ggtacctcgc gagcctaggg ctagctggat ccgccaccat gcctgagcct
     1921 gctaaatctg cccctgctcc taagaagggc agcaagaaag ccgtgacaaa ggcccagaag
     1981 aaggacggca agaagcggaa gcggagccgg aaagaaagct acagcgtgta cgtgtacaag
     2041 gtgctgaaac aggtgcaccc cgacaccggc atcagctcta aggccatggg catcatgaac
     2101 agcttcgtga acgacatctt cgagcggatt gccggcgagg cctctagact ggcccactac
     2161 aacaagcgga gcaccatcac cagccgcgag attcagacag ccgtcagact tctgctgcct
     2221 ggcgaactgg ccaaacacgc tgtgtctgag ggaacaaagg ccgtgaccaa gtacaccagc
     2281 agcaagcctc caccagccgg caagcccatt cctaatcctc tgctgggcct cgacagcacc
     2341 ggaagatctg ccatggtgtc taagggcgaa gccgtcatca aagaattcat gcggttcaag
     2401 gtgcacatgg aaggcagcat gaacggccac gagttcgaga tcgaaggcga aggcgagggc
     2461 agaccttatg agggcacaca gaccgccaag ctgaaagtga caaaaggcgg ccctctgcct
     2521 ttcagctggg acattctgag ccctcagttt atgtacggca gccgggcctt cacaaagcac
     2581 cctgccgata ttcccgacta ctacaagcag agcttccccg agggcttcaa gtgggagaga
     2641 gtgatgaact tcgaggacgg cggagccgtg accgtgacac aggatacaag cctggaagat
     2701 ggcaccctga tctacaaagt gaagctgcgg ggcaccaact ttccacctga tggccccgtg
     2761 atgcagaaaa agaccatggg ctgggaagcc agcaccgaga gactgtatcc tgaggatggc
     2821 gtgctgaagg gcgacatcaa gatggccctg agactgaagg atggcggcag atacctggcc
     2881 gacttcaaga ccacctacaa ggccaagaaa cccgtgcaga tgcctggcgc ctacaacgtg
     2941 gacagaaagc tggacatcac aagccacaac gaggactaca ccgtggtgga acagtacgag
     3001 cggagcgaag gcagacactc tacaggcgga atggacgagc tgtacaaatg agatatcctc
     3061 gagaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3121 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3181 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3241 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3301 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3361 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3421 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3481 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3541 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3601 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3661 ccgcatcgat accgtcgacc cgggcggccg cttcgagcag acatgataag atacattgat
     3721 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3781 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3841 tgcattcatt ttatgtttca ggttcaggag gagatgtggg aggtttttta aagcaagtaa
     3901 aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa
     3961 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     4021 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4081 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa
     4141 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4201 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4261 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4321 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4381 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4441 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4501 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4561 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4621 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4681 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4741 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4801 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4861 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4921 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4981 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac c
//
