LOCUS       sequence-380160-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-BiFC-ED2
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        445..789
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(932..934,935..937,938..1450)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(977..998)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1238..1257)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        1650..1752
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1783..2088
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2445..3128)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(3501..3522)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3711..4016)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(4047..4149)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(4472..4816)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5235..5251)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5274..5290)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5274..5290)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5327..5347)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5327..5345)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5366..5382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5366..5382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5379..5401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5390..5406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5414..5420,5421..5438,5439..5444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5459..5480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5597..5614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5719..5743
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5894..6329
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(6609..6628)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6620..7435
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6620..7435
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6693..6712)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7536..8124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8025..8044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8191..8213
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(8237..8489)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8529..9323)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8640..8659)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9250..9269
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9357..9536)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     9783..9799
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9809..9828
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9809..9827
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9852..9868
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9853..9869
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1141 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1381 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1441 caacatcgag gactccggac tcagatctca gtggtctctg tccagtcctg gcctcgtcgg
     1501 ccattctcga ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     1561 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     1621 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1681 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1741 aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gggccagtct ggccacttgt ggtctgctga gaccctgatc tagagtccgc
     2161 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     2221 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     2281 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     2341 attcctaaaa ccaaaatcca gtgacgctta tgggaccgac atatcagtat atattcttat
     2401 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     2461 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2521 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2581 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2641 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2701 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2761 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2821 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2881 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     2941 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     3001 gccgtaatat ccagctgaac ggtctggtta taggtacatt gaacaactga ctgaaatgcc
     3061 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     3121 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     3181 agtgatctta tttcattatg gtgaaagttg gaacctctta ccgggcgtca ctggattttg
     3241 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     3301 ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg
     3361 ggaactactc acacattatt ctggagaaaa atagagagag atagatttgt agagagagac
     3421 tggtgatttt tgcggactct agatcaggtg gatcttcact tgtacagctc gtccatgccg
     3481 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     3541 gggtctttgc tcagggcgga ctggtagctc aggtagtggt tgtcgggcag cagcacgggg
     3601 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccccactgg
     3661 aaacatccag gtctggccag actggccctg tgtataaggg agcctgacat ttatattccc
     3721 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     3781 tcttccccga taacggatac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     3841 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
     3901 gcactggcca gggggatcac catccgtcgc ccaggcgtgt caataatatc actctgtaca
     3961 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     4021 ccagtccctg ttctcgtcag caaaagttta gcttccttag ctcctgaaaa tctcgataac
     4081 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     4141 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     4201 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     4261 agtgcgtcgg gtgatgctgc caacttagtc gagaatggcc gacgaggcca cgcatggtcg
     4321 agccaactct gctatcgttc gtaaatggtg aaaattttca gaaaattgct tttgctttaa
     4381 aagaaatgat ttaaattgct gcaatagaag tagaatgctt gattgcttga gattcgtttg
     4441 ttttgtatat gttgtgttga gaactctcga cgtcctctcc aaatgaaatg aacttcctta
     4501 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     4561 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     4621 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttcaacgat
     4681 ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt ccactatctt
     4741 cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg atattaccct
     4801 ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga tatttttgga
     4861 gtagacaagt gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt
     4921 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     4981 actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta
     5041 ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa
     5101 tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc
     5161 tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca catgttgacc
     5221 ggtggcgcgc cggcactggc cgtcgtttta caacgaattc ctgcagcccg ggggatccac
     5281 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5341 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5401 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5461 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5521 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5581 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5641 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5701 gaaagaacat gaaggccttg acaggatata ttggcgggta aactaagtcg ctgtatgtgt
     5761 ttgtttgaga tctaattccg gggatcggaa atccagaagc ccgagaggtt gccgcctttc
     5821 gggctttttc tttttcaaaa aaaaaaattt ataaaacgat ctgttgcggc cggccgccgg
     5881 gttgtgggca aaggcgctcg acggtgggca accgcttgcg gttgtccacg ggcggagccg
     5941 gtgcgcgtag cgcattgtcc acaagccaag ggcgaccaat aattgatata tatattcata
     6001 attgaaaagc taattgaaca tactacttgc tgtaactact tgccggagcg aggggtgttt
     6061 gcaagctgtt gatctgaaag ggctattagc gttctcacgt gcctttttga ttagcgattt
     6121 cacgtgacct tattagcgat ttcacgtact ccgattagcg atttcacgta ccctgattag
     6181 cgatttcacg tggatagttt ttggagcggg ccggaaagcc ccgtgaatca aggctttgcg
     6241 gggcattagc ggtttcacgt ggataactac cctctatcca caggcttccg gggataaaaa
     6301 agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat catccagcca
     6361 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
     6421 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
     6481 caactcagca aaagttcgat ttattcaaca aagccacgtt gtggctcaaa atctctgatg
     6541 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     6601 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct caaggccgcg
     6661 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     6721 gcaatcaggt gcgacaatct accgattgta tgggaagccc gatgcgccag agttgtttct
     6781 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     6841 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     6901 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
     6961 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     7021 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     7081 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     7141 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     7201 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     7261 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     7321 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     7381 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     7441 attggttaat tggttgtaac catgaccaaa atcccttaac gtgagttttc gttccactga
     7501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8161 ttttgctggc cttttgctca catgagatct tggcaggata tattgtggtg taacgttatc
     8221 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     8281 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     8341 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     8401 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     8461 aaactttatt gccaaatgtt tgaacgatct gcttgactct agctagagtc cgaaccccag
     8521 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     8581 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     8641 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     8701 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgccg
     8761 tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg
     8821 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     8881 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     8941 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     9001 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     9061 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     9121 gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca
     9181 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     9241 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     9301 tgcaatccat cttgttcaat catgcctcga tcgagttgag agtgaatatg agactctaat
     9361 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     9421 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     9481 tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg cggttctgtc
     9541 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     9601 taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc cattcaggct
     9661 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     9721 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     9781 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     9841 cgggcccccc ctcgaggtcg acggtatcga t
//
