LOCUS       sequence-380175-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-BiFC-ED3
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        445..789
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        1112..1214
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1245..1550
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             join(1608..1610,1611..1613,1614..2126)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(1653..1674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1914..1933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(2441..3124)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     CDS             complement(3500..3805)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(3836..3938)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4177..4198)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4478..4822)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5241..5257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5280..5296)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5280..5296)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5333..5353)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5333..5351)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5372..5388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5372..5388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5385..5407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5396..5412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5420..5426,5427..5444,5445..5450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5465..5486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5603..5620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5725..5749
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5900..6335
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(6615..6634)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6626..7441
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6626..7441
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6699..6718)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7542..8130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8031..8050
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8197..8219
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(8243..8495)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8535..9329)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8646..8665)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9256..9275
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9363..9542)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     9789..9805
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9815..9834
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9815..9833
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9858..9874
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9859..9875
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc cagtggtctc tgtccagtcc tggcctcgtc
      961 ggccattctc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     1021 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     1081 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     1141 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1201 ctaaggaagc taaacttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     1261 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     1321 acacgcctgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     1381 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     1441 ccgatatggc cagtgtgccg gtatccgtta tcggggaaga agtggctgat ctcagccacc
     1501 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     1561 cccttataca cagggccagt ctggccactt gtggtctgct gagacccatg gtgagcaagg
     1621 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1681 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1741 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1801 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     1861 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1921 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1981 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2041 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2101 acttcaagat ccgccacaac atcgaggact gaagatccac ctgatctaga gtccgcaaaa
     2161 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     2221 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     2281 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     2341 ctaaaaccaa aatccagtga cgcttatggg accgacatat cagtatatat tcttataccg
     2401 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatccacgc
     2461 ggcgtttacg ccccccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2521 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2581 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2641 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     2701 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2761 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2821 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2881 gctcaccgtc tttcattgcc atacggaact ccggatgagc attcatcagg cgggcaagaa
     2941 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3001 taatatccag ctgaacggtc tggttatagg tacattgaac aactgactga aatgcctcaa
     3061 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3121 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3181 atcttatttc attatggtga aagttggaac ctcttaccgg gcgtcactgg attttggttt
     3241 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     3301 tcttatatgc tcaacacatg agcgaaaccc tataagaacc ctaattccct tatctgggaa
     3361 ctactcacac attattctgg agaaaaatag agagagatag atttgtagag agagactggt
     3421 gatttttgcg gactctagat caccactgga aacatccagg tctggccaga ctggccctgt
     3481 gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt
     3541 cattttcgcg gtggctgaga tcagccactt cttccccgat aacggatacc ggcacactgg
     3601 ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt
     3661 cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc
     3721 caggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt
     3781 tataggtgta aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagtttag
     3841 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     3901 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     3961 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     4021 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttagtcg
     4081 agaatggccg acgaggccac gcatggtcga gccaactcta gatctgagtc cggacttgta
     4141 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     4201 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
     4261 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     4321 cacgctgccg cccatggcta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg
     4381 ctttaaaaga aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt
     4441 cgtttgtttt gtatatgttg tgttgagaac tctcgacgtc ctctccaaat gaaatgaact
     4501 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     4561 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     4621 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc
     4681 aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac
     4741 tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat
     4801 taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt
     4861 tttggagtag acaagtgtgt cgtgctccac catgttatca catcaatcca cttgctttga
     4921 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     4981 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
     5041 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
     5101 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
     5161 gtcttctgag actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccacatg
     5221 ttgaccggtg gcgcgccggc actggccgtc gttttacaac gaattcctgc agcccggggg
     5281 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     5341 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5701 acgcaggaaa gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt
     5761 atgtgtttgt ttgagatcta attccgggga tcggaaatcc agaagcccga gaggttgccg
     5821 cctttcgggc tttttctttt tcaaaaaaaa aaatttataa aacgatctgt tgcggccggc
     5881 cgccgggttg tgggcaaagg cgctcgacgg tgggcaaccg cttgcggttg tccacgggcg
     5941 gagccggtgc gcgtagcgca ttgtccacaa gccaagggcg accaataatt gatatatata
     6001 ttcataattg aaaagctaat tgaacatact acttgctgta actacttgcc ggagcgaggg
     6061 gtgtttgcaa gctgttgatc tgaaagggct attagcgttc tcacgtgcct ttttgattag
     6121 cgatttcacg tgaccttatt agcgatttca cgtactccga ttagcgattt cacgtaccct
     6181 gattagcgat ttcacgtgga tagtttttgg agcgggccgg aaagccccgt gaatcaaggc
     6241 tttgcggggc attagcggtt tcacgtggat aactaccctc tatccacagg cttccgggga
     6301 taaaaaagcc cgctcgacgg cgggctgttg gatggggatc gcctgaatcg ccccatcatc
     6361 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt
     6421 gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg
     6481 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgg ctcaaaatct
     6541 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     6601 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcaag
     6661 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     6721 tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt
     6781 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     6841 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     6901 tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga
     6961 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     7021 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     7081 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     7141 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     7201 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     7261 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     7321 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     7381 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     7441 atcagaattg gttaattggt tgtaaccatg accaaaatcc cttaacgtga gttttcgttc
     7501 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7561 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7621 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7681 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7741 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7801 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7861 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7921 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7981 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8041 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8101 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8161 ctggcctttt gctggccttt tgctcacatg agatcttggc aggatatatt gtggtgtaac
     8221 gttatcagct tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta
     8281 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     8341 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     8401 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     8461 cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctagct agagtccgaa
     8521 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     8581 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     8641 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     8701 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     8761 tcgccgtggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac
     8821 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8881 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     8941 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     9001 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     9061 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     9121 agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc
     9181 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     9241 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     9301 cctgcgtgca atccatcttg ttcaatcatg cctcgatcga gttgagagtg aatatgagac
     9361 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
     9421 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
     9481 tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt
     9541 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
     9601 ctcccttaat tctccgctca tgatcgataa cattaacgct tacaatttcc attcgccatt
     9661 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     9721 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     9781 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
     9841 gggtaccggg ccccccctcg aggtcgacgg tatcgat
//
