LOCUS       sequence-380183-        9881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-BiFC-ED1
FEATURES             Location/Qualifiers
     source          1..9881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        445..789
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(932..934,935..937,938..1450)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(977..998)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1238..1257)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        1650..1752
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1783..2088
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(2445..3128)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     CDS             complement(3504..3809)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(3840..3942)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4181..4202)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4482..4826)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5245..5261)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5284..5300)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5284..5300)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5337..5357)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5337..5355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5376..5392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5376..5392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5389..5411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5400..5416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5424..5430,5431..5448,5449..5454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5469..5490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5607..5624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5729..5753
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5904..6339
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(6619..6638)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6630..7445
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6630..7445
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6703..6722)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7546..8134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8035..8054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8201..8223
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(8247..8499)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8539..9333)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8650..8669)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9260..9279
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9367..9546)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     9793..9809
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9819..9838
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9819..9837
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9862..9878
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9863..9879
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1141 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1381 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1441 caacatcgag gactccggac tcagatctca gtggtctctg tccagtcctg gcctcgtcgg
     1501 ccattctcga ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     1561 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     1621 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1681 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1741 aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gggccagtct ggccacttgt ggtctgctga gaccctgatc tagagtccgc
     2161 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     2221 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     2281 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     2341 attcctaaaa ccaaaatcca gtgacgctta tgggaccgac atatcagtat atattcttat
     2401 accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta agccggatcc
     2461 acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc attaagcatt
     2521 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2581 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2641 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     2701 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2761 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2821 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2881 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
     2941 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     3001 gccgtaatat ccagctgaac ggtctggtta taggtacatt gaacaactga ctgaaatgcc
     3061 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     3121 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     3181 agtgatctta tttcattatg gtgaaagttg gaacctctta ccgggcgtca ctggattttg
     3241 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     3301 ggtttcttat atgctcaaca catgagcgaa accctataag aaccctaatt cccttatctg
     3361 ggaactactc acacattatt ctggagaaaa atagagagag atagatttgt agagagagac
     3421 tggtgatttt tgcggactct agatcaccac tggaaacatc caggtctggc cagactggcc
     3481 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
     3541 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga taccggcaca
     3601 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
     3661 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
     3721 cgcccaggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
     3781 cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt cagcaaaagt
     3841 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3901 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa
     3961 aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag
     4021 tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta
     4081 gtcgagaatg gccgacgagg ccacgcatgg tcgagccaac tctagatctg agtccggact
     4141 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4201 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc aggtagtggt
     4261 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4321 gctgcacgct gccgcccatg gctatcgttc gtaaatggtg aaaattttca gaaaattgct
     4381 tttgctttaa aagaaatgat ttaaattgct gcaatagaag tagaatgctt gattgcttga
     4441 gattcgtttg ttttgtatat gttgtgttga gaactctcga cgtcctctcc aaatgaaatg
     4501 aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta
     4561 cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt
     4621 tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat
     4681 cttcaacgat ggcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt
     4741 ccactatctt cacaataaag tgacagatag ctgggcaatg gaatccgagg aggtttccgg
     4801 atattaccct ttgttgaaaa gtctcaattg ccctttggtc ttctgagact gtatctttga
     4861 tatttttgga gtagacaagt gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct
     4921 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     4981 ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat
     5041 ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg
     5101 ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc
     5161 tttggtcttc tgagactgta tctttgatat ttttggagta gacaagtgtg tcgtgctcca
     5221 catgttgacc ggtggcgcgc cggcactggc cgtcgtttta caacgaattc ctgcagcccg
     5281 ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     5341 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5701 gataacgcag gaaagaacat gaaggccttg acaggatata ttggcgggta aactaagtcg
     5761 ctgtatgtgt ttgtttgaga tctaattccg gggatcggaa atccagaagc ccgagaggtt
     5821 gccgcctttc gggctttttc tttttcaaaa aaaaaaattt ataaaacgat ctgttgcggc
     5881 cggccgccgg gttgtgggca aaggcgctcg acggtgggca accgcttgcg gttgtccacg
     5941 ggcggagccg gtgcgcgtag cgcattgtcc acaagccaag ggcgaccaat aattgatata
     6001 tatattcata attgaaaagc taattgaaca tactacttgc tgtaactact tgccggagcg
     6061 aggggtgttt gcaagctgtt gatctgaaag ggctattagc gttctcacgt gcctttttga
     6121 ttagcgattt cacgtgacct tattagcgat ttcacgtact ccgattagcg atttcacgta
     6181 ccctgattag cgatttcacg tggatagttt ttggagcggg ccggaaagcc ccgtgaatca
     6241 aggctttgcg gggcattagc ggtttcacgt ggataactac cctctatcca caggcttccg
     6301 gggataaaaa agcccgctcg acggcgggct gttggatggg gatcgcctga atcgccccat
     6361 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt
     6421 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga
     6481 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt gtggctcaaa
     6541 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     6601 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
     6661 caaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     6721 ataatgtcgg gcaatcaggt gcgacaatct accgattgta tgggaagccc gatgcgccag
     6781 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     6841 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     6901 ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag
     6961 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     7021 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     7081 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     7141 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     7201 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     7261 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     7321 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     7381 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     7441 tctaatcaga attggttaat tggttgtaac catgaccaaa atcccttaac gtgagttttc
     7501 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7561 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7621 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7681 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7741 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7801 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7921 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7981 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8161 gttcctggcc ttttgctggc cttttgctca catgagatct tggcaggata tattgtggtg
     8221 taacgttatc agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa
     8281 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     8341 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     8401 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     8461 caatcttaag aaactttatt gccaaatgtt tgaacgatct gcttgactct agctagagtc
     8521 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     8581 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     8641 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     8701 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     8761 ggcatcgccg tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc
     8821 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     8881 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     8941 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     9001 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     9061 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     9121 ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc
     9181 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     9241 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     9301 agaacctgcg tgcaatccat cttgttcaat catgcctcga tcgagttgag agtgaatatg
     9361 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     9421 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     9481 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     9541 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     9601 gtgactccct taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc
     9661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9721 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9781 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     9841 aattgggtac cgggcccccc ctcgaggtcg acggtatcga t
//
