LOCUS       sequence-380186-        3118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to produce ISH probe for mMyh1 gene with UTR sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Tanji M, Wada K, Sakamoto K, Ono Y, Inui M
  TITLE     Digoxigenin-labeled RNA probes for untranslated regions enable the 
            isoform-specific gene expression analysis of myosin heavy chains in 
            whole-mount in situ hybridization.
  JOURNAL   Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832.
  PUBMED    36517457
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Growth 
            Differ. 2022 Dec 14. doi: 10.1111/dgd.12832."
COMMENT     Sequence Label: pGEMT-mMyh1UTR
FEATURES             Location/Qualifiers
     source          1..3118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      420..1008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     909..928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1162..1179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1296..1317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1332..1337,1338..1355,1356..1362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1370..1386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1375..1397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1394..1410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1394..1410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1428..1446
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1428..1445
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1643..1652
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1647..1656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1686..1705)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1687..1705)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1712..1729)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1712..1728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1721..1743)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1869..2324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1956..1975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2166..2187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2402..2506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2507..2575,2576..3118,1..249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2725..2744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
       61 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1261 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1321 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1381 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac
     1441 tatagaatac tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgacctgc
     1501 aggcggccgc gaattcacta gtgattggca gataaatttt ttatctccca aagttatgag
     1561 tacaaaacag agtgacaaag atcttcacat tttgctcatc tctttggtca ctttcctgca
     1621 cttggatcat cgaattcccg cggccgccat ggcggccggg agcatgcgac gtcgggccca
     1681 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     1741 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1801 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1861 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1921 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1981 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2041 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2101 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2161 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2221 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2281 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt
     2341 ctccttacgc atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt
     2401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2701 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3001 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaa
//
