LOCUS       sequence-380187-        9867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nimble cloning-compatible vector for plant BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-BiFC-ED4
FEATURES             Location/Qualifiers
     source          1..9867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        445..789
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        1112..1214
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1245..1550
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             join(1608..1610,1611..1613,1614..2126)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(1653..1674)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1914..1933)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2460..3143
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVVQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(3497..3518)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3707..4012)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     promoter        complement(4043..4145)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(4468..4812)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(5231..5247)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5270..5286)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5270..5286)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5323..5343)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5323..5341)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5362..5378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5362..5378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5375..5397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5386..5402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5410..5416,5417..5434,5435..5440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5455..5476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5593..5610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5715..5739
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5890..6325
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(6605..6624)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6616..7431
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6616..7431
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6689..6708)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7532..8120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8021..8040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    8187..8209
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(8233..8485)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8525..9319)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8636..8655)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9246..9265
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9353..9532)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     9779..9795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9805..9824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9805..9823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9848..9864
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9849..9865
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc cagtggtctc tgtccagtcc tggcctcgtc
      961 ggccattctc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg
     1021 tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc
     1081 cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc
     1141 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
     1201 ctaaggaagc taaacttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     1261 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     1321 acacgcctgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     1381 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     1441 ccgatatggc cagtgtgccg gtatccgtta tcggggaaga agtggctgat ctcagccacc
     1501 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     1561 cccttataca cagggccagt ctggccactt gtggtctgct gagacccatg gtgagcaagg
     1621 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1681 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1741 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1801 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     1861 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1921 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1981 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2041 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2101 acttcaagat ccgccacaac atcgaggact gaagatccac ctgatctaga gtccgcaaaa
     2161 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     2221 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     2281 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     2341 ctaaaaccaa aatccagtga cgcccggtaa gaggttccaa ctttcaccat aatgaaataa
     2401 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2461 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2521 attttgaggc atttcagtca gttgttcaat gtacctataa ccagaccgtt cagctggata
     2581 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2641 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
     2701 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2761 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2821 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2881 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2941 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3001 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     3061 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggg gggcgtaaac
     3121 gccgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     3181 ttgcggtata agaatatata ctgatatgtc ggtcccataa gcgtcactgg attttggttt
     3241 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     3301 tcttatatgc tcaacacatg agcgaaaccc tataagaacc ctaattccct tatctgggaa
     3361 ctactcacac attattctgg agaaaaatag agagagatag atttgtagag agagactggt
     3421 gatttttgcg gactctagat caggtggatc ttcacttgta cagctcgtcc atgccgagag
     3481 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     3541 ctttgctcag ggcggactgg tagctcaggt agtggttgtc gggcagcagc acggggccgt
     3601 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccc cactggaaac
     3661 atccaggtct ggccagactg gccctgtgta taagggagcc tgacatttat attccccaga
     3721 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
     3781 ccccgataac ggataccggc acactggcca tatcggtggt catcatgcgc cagctttcat
     3841 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
     3901 tggccagggg gatcaccatc cgtcgcccag gcgtgtcaat aatatcactc tgtacatcca
     3961 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
     4021 tccctgttct cgtcagcaaa agtttagctt ccttagctcc tgaaaatctc gataactcaa
     4081 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4141 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4201 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     4261 cgtcgggtga tgctgccaac ttagtcgaga atggccgacg aggccacgca tggtcgagcc
     4321 aactctgcta tcgttcgtaa atggtgaaaa ttttcagaaa attgcttttg ctttaaaaga
     4381 aatgatttaa attgctgcaa tagaagtaga atgcttgatt gcttgagatt cgtttgtttt
     4441 gtatatgttg tgttgagaac tctcgacgtc ctctccaaat gaaatgaact tccttatata
     4501 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
     4561 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     4621 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc
     4681 tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca
     4741 ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt
     4801 tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag
     4861 acaagtgtgt cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt
     4921 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     4981 tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag
     5041 ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg
     5101 aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag
     5161 actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccacatg ttgaccggtg
     5221 gcgcgccggc actggccgtc gttttacaac gaattcctgc agcccggggg atccactagt
     5281 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     5341 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5401 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5461 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5521 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5701 gaacatgaag gccttgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt
     5761 ttgagatcta attccgggga tcggaaatcc agaagcccga gaggttgccg cctttcgggc
     5821 tttttctttt tcaaaaaaaa aaatttataa aacgatctgt tgcggccggc cgccgggttg
     5881 tgggcaaagg cgctcgacgg tgggcaaccg cttgcggttg tccacgggcg gagccggtgc
     5941 gcgtagcgca ttgtccacaa gccaagggcg accaataatt gatatatata ttcataattg
     6001 aaaagctaat tgaacatact acttgctgta actacttgcc ggagcgaggg gtgtttgcaa
     6061 gctgttgatc tgaaagggct attagcgttc tcacgtgcct ttttgattag cgatttcacg
     6121 tgaccttatt agcgatttca cgtactccga ttagcgattt cacgtaccct gattagcgat
     6181 ttcacgtgga tagtttttgg agcgggccgg aaagccccgt gaatcaaggc tttgcggggc
     6241 attagcggtt tcacgtggat aactaccctc tatccacagg cttccgggga taaaaaagcc
     6301 cgctcgacgg cgggctgttg gatggggatc gcctgaatcg ccccatcatc cagccagaaa
     6361 gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac
     6421 ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac
     6481 tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgg ctcaaaatct ctgatgttac
     6541 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     6601 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcaag gccgcgatta
     6661 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     6721 tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     6781 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     6841 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     6901 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
     6961 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     7021 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     7081 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     7141 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     7201 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     7261 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     7321 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     7381 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     7441 gttaattggt tgtaaccatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7501 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7561 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7621 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7681 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7741 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7801 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7861 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7921 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7981 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8041 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8101 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8161 gctggccttt tgctcacatg agatcttggc aggatatatt gtggtgtaac gttatcagct
     8221 tgcatgccgg tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     8281 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     8341 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     8401 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     8461 tttattgcca aatgtttgaa cgatctgctt gactctagct agagtccgaa ccccagagtc
     8521 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     8581 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     8641 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     8701 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccgtggg
     8761 tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac agttcggctg
     8821 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     8881 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     8941 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     9001 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     9061 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     9121 gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     9181 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     9241 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     9301 atccatcttg ttcaatcatg cctcgatcga gttgagagtg aatatgagac tctaattgga
     9361 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
     9421 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     9481 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
     9541 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     9601 tctccgctca tgatcgataa cattaacgct tacaatttcc attcgccatt caggctgcgc
     9661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     9721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     9781 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg
     9841 ccccccctcg aggtcgacgg tatcgat
//
