LOCUS       sequence-380200-        5976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MBD domain of MBD1 fused to C-terminal part of split mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A
  TITLE     Modular fluorescence complementation sensors for live cell detection
            of epigenetic signals at endogenous genomic sites.
  JOURNAL   Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
  PUBMED    28935858
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-21"; volume: "8(1):649. doi"; pages: " 
            10.1038/s41467-017-00457-z"
COMMENT     Sequence Label: pcDNA3 Flag-MBD1-14-mVenusC
FEATURES             Location/Qualifiers
     source          1..5976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(224..242)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(225..242)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(262..279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    268..492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      538..966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(625..644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     835..856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(975..995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        980..1309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1160..1295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1222..1241
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1376..2170
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1430..1449)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2040..2059
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2344..2465
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2381..2400)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2435..2454
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2514..2530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2514..2530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2527..2549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2538..2554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2562..2568,2569..2586,2587..2592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2607..2628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2745..2762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2916..3504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2996..3015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3675..4466,4467..4535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4298..4317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4536..4640)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4715..4734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4906..5285
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5286..5489
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5440..5460
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5534..5553
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5534..5552
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5648..5713
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gccttcaagg ccagccaaga ctcggaaacg tcaggttgga ccccagagtg gtgaggtcag
       61 gaaggaggcc ccgatcgatg gtggcggtgg ctctggaggt ggtgggtcct ccggagaccc
      121 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      181 cggcatggac gagctgtaca agtaataatc tagagggccc tattctatag tgtcacctaa
      241 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      301 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      361 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      421 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
      481 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
      541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
      841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      961 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     1021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     1081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     1141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     1201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     1261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     1321 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     1381 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     1441 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     1501 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     1561 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     1621 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     1681 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     1741 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     1801 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     1861 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     1921 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     1981 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     2041 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     2101 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     2161 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     2221 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     2281 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     2341 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2401 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2461 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     2521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2581 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2641 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     4681 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4741 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     4801 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     4861 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     4921 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4981 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     5041 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     5101 aatgggtgga gtatttacga taaactgccc acttggcagt acatcaagtg tatcatatgc
     5161 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     5221 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     5281 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5341 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5401 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5461 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     5521 ggcttatcga aattaatacg actcactata gggagaccca agcttctcgg gcccaataat
     5581 cattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     5641 tataatggac tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga
     5701 cgatgacgat aagatggctg aggactggct ggactgcccg gccctgggcc ctggctggaa
     5761 gcgccgcgaa gtctttcgca agtcaggggc cacctgtgga cgctcagaca cctattacca
     5821 gagccccaca ggagacagga tccgaagcaa agttgagctg actcgatacc tgggccctgc
     5881 gtgtgatctc accctcttcg acttcaaaca aggcatcttg tgctatccag cccccaaggc
     5941 ccatcccgtg gcggttgcca gcaagaagcg aaagaa
//
