LOCUS       sequence-380201-        3121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to produce ISH probe for mMyh3 gene with UTR sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Tanji M, Wada K, Sakamoto K, Ono Y, Inui M
  TITLE     Digoxigenin-labeled RNA probes for untranslated regions enable the 
            isoform-specific gene expression analysis of myosin heavy chains in 
            whole-mount in situ hybridization.
  JOURNAL   Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832.
  PUBMED    36517457
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Growth 
            Differ. 2022 Dec 14. doi: 10.1111/dgd.12832."
COMMENT     Sequence Label: pGEMT-mMyh3UTR
FEATURES             Location/Qualifiers
     source          1..3121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      467..1055
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     956..975
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1209..1226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1343..1364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1379..1384,1385..1402,1403..1409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1417..1433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1422..1444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1441..1457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1441..1457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1475..1493
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1475..1492
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1693..1702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1697..1706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1736..1755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1737..1755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1762..1779)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1762..1778)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1771..1793)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1919..2374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2006..2025)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2216..2237
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2452..2556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2557..2625,2626..3121,1..296)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2775..2794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
       61 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
      121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
      181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
      241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
      301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
      361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
      421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      601 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1081 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1141 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     1201 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     1261 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1321 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     1381 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     1441 caggaaacag ctatgaccat gattacgcca agctatttag gtgacactat agaatactca
     1501 agctatgcat ccaacgcgtt gggagctctc ccatatggtc gacctgcagg cggccgcgaa
     1561 ttcactagtg attccaactt gaaacaaggc aaactttatt gcatgtggaa aagtgatacg
     1621 tggaaattaa gtagaattgt caggagccac gaaaatatac attctgcata tcttctgaat
     1681 cgaattcccg cggccgccat ggcggccggg agcatgcgac gtcgggccca attcgcccta
     1741 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1801 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1861 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
     1921 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1981 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2041 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2101 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2161 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2221 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2281 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2341 gcgaatttta acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     2401 atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2461 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2521 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2581 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     2641 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     2701 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     2761 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     2821 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     2881 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     2941 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3001 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3061 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3121 c
//
