LOCUS       sequence-380206-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter 
            with mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
  TITLE     Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic 
            Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used 
            for Image Segmentation.
  JOURNAL   Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
  PUBMED    30088945
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Assay Drug 
            Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862."
COMMENT     Sequence Label: pLVX-ATF4 mScarlet NLS
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        24..327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        328..531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     482..502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     528..552
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      736..745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1022..1048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1022..1048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1784..1810
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        1830..2329
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1865..1888)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2262..2281
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2350..2949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2350..2369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2846..2866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2963..3551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3016..3036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3434..3445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3758..4391
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4519..4535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4519..4535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4532..4554)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4543..4559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4567..4573,4574..4591,4592..4597))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4612..4633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4750..4767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4921..5509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5001..5020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5680..6471,6472..6540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6303..6322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6541..6645)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6693..6827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6730..6749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6784..6803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6995..7628
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7675..7800
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8297..8530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8715..8759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9022..9137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
       61 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      121 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      181 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      241 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      301 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      361 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      421 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      481 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      541 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
      601 ctctactaga ggatcgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
      661 ctactttgcc cgcccacaga tgtagttttc tctgcgcgtg tgcgttttcc ctcctccccg
      721 ccctcagggt ccacggccac catggcgtat taggggcagc agtgcctgcg gcagcattgg
      781 cctttgcagc ggcggcagca gcaccaggct ctgcagcggc aacccccagc ggcttaagcc
      841 atggcgcttc tcacggcatt cagcagcagc gttgctgtaa ccgacaaaga caccttcgaa
      901 ttaagcacat tcctcgattc cagcaaagca ccgcaacatg accgaaatga gcttcctgag
      961 cagcgaggtg ttggtggggg acttgatgtc ccccttcgac cagtcgggtt tgggggctga
     1021 atacccctac gacgtgcccg actacgccgg tggatcaggg ggaggatccg tctccaaagg
     1081 ggaagcggtc attaaagagt tcatgaggtt caaagttcat atggagggca gtatgaacgg
     1141 ccatgaattt gaaatcgagg gcgaggggga gggacgccct tacgaaggga cacaaacagc
     1201 caaactgaag gttacgaaag gtgggccact tccctttagt tgggatatac tgtcacctca
     1261 atttatgtac ggcagccgag ccttcattaa acatcccgct gacattccgg attactataa
     1321 gcagtccttt ccagaggggt ttaaatggga gcgggttatg aatttcgagg atggcggggc
     1381 cgtaactgtc acgcaggata ccagtctgga agatggtacc ctgatttata aggttaagct
     1441 cagaggaacg aacttcccgc cagatggtcc cgtaatgcaa aaaaagacga tgggttggga
     1501 ggcatcaact gaaagattgt atcctgagga tggtgtcctt aagggtgata ttaaaatggc
     1561 tctgaggctg aaggatggcg gaaggtattt ggcagatttc aaaactacat ataaggccaa
     1621 gaagcctgtt cagatgccag gtgcctataa tgtggacaga aaacttgaca ttacatcaca
     1681 caacgaggat tataccgtcg tggagcaata tgagagaagt gagggccgac atagtaccgg
     1741 cggcatggat gaactctata aggtcgacgg tggatcaggg ggacccgccg ccaagcgcgt
     1801 gaagctggac taatctagat aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     1861 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     1921 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     1981 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     2041 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     2101 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     2161 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     2221 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     2281 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     2341 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     2401 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     2461 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     2521 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     2581 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     2641 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     2701 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     2761 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     2821 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     2881 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     2941 ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac
     3001 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3061 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3121 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3181 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3241 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3301 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     3361 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3421 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3481 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3541 cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca
     3601 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     3661 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     3721 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     3781 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     3841 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     3901 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     3961 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     4021 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     4081 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     4141 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     4201 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4261 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4321 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4381 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     4441 atgaatatca gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta
     4501 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4621 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6661 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     6721 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6781 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     6841 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     6901 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     6961 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     7021 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     7081 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     7141 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     7201 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     7261 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     7321 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     7381 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     7441 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7501 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7561 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7621 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7681 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7741 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     7801 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     7861 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     7921 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     7981 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8041 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8101 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     8161 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8221 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8281 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8341 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     8401 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8461 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8521 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8581 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8641 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8701 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8761 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     8821 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     8881 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     8941 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     9001 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     9061 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9121 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt t
//
