LOCUS       sequence-380211-        9295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS , C-terminal HA ) 
            w/ mutations stopping S phase degradation and in MCM binding 
            (R198A/R210A), expressed from all-in-one pCW vector (TetOn + rtTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pCW-ND-Cdt1-Delta-R198A/R210A-HA-Puro
FEATURES             Location/Qualifiers
     source          1..9295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2163..2183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2196..2222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2237..2747
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2666..2684
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2757..3353
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2757..2776)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3253..3273
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3354..3407
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3408..4025,4026..4031,4032..4067,4068..4070,4071..4106,
                     4107..4109,4110..4145,4146..4154)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4194..4214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4612..4623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4828..5061
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5133..5254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5170..5189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5224..5243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5294..5429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5429..5500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5501..5569,5570..6361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5719..5738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6532..7120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7021..7040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7274..7291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7408..7429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7444..7449,7450..7467,7468..7474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7482..7498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7487..7509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7506..7522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7506..7522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7541..7561
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7543..7561
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7589..7815
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7816..7996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8043..8168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8661..8894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9079..9123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gatagctccg ccaaagctcg cttgtcggac tccctctccg gctagaccgg
      601 cacttcgcgc ccccgcctcc gctacttcag gtagccgaaa gagggcgagg ccaccagcag
      661 cccccggacg cgatcaggcg aggccacctg ctcgagcggc tgctcgcctc tcagtagatg
      721 aggttagcag ccccagtacc cccgaggccc cagacatccc agcctgccct tctccgggcc
      781 agaagataaa gaaatccacc ccggcagcag gtcagccgcc ccacctgaca tccgcgcagg
      841 accaggacac catctctgag cttgcgtcat gcctgcaacg ggcccgggag ctgggggcaa
      901 gagtccgggc gctgaaggcc agtgcccagg atgctgggga gtcctgcacc ccagaggccg
      961 agggccgccc tgaggagcca tgtggcgaga aggcgcccgc ctaccagcgc ttccatgccc
     1021 tggcccagcc cggcctgccg ggactcgtgc tgccctacaa gtaccaggtg ctggcggaga
     1081 tgttcgccag catggacacc atcgtgggca tgctccacaa cgcctccgag acgcccacct
     1141 ttgccaaggt ccagcggggc gtccaggaca tgatgcgtag gcgttttgag gagcgcaatg
     1201 ttggccagat caaaaccgtg tacccggcct cctaccgctt ccgccaggag cgcagtgtcc
     1261 ccaccttcaa ggatggcacc aggaggtcag attaccagct caccatcgag ccactgctgg
     1321 agcaggaggc tgacggagca gccccccagc tcacggcctc gcgcctcctg cagcgacggc
     1381 agatcttcag ccagaagctg gtggagcatg tcaaggagca ccacaaggcc ttcctggcct
     1441 ccctgagccc cgccatggtg gtgccggagg accagctgac ccgctggcac ccgcgcttca
     1501 acgtggatga agtacccgac atcgagccgg ccgcgctgcc ccagccaccc gccacggaga
     1561 agctcaccac tgctcaggag gtgctggccc gggcccgcaa cctgatttca cccaggatgg
     1621 agaaggcctt gagtcaattg gccctgcgct ctgctgcgcc cagcagcccc gggtctccca
     1681 ggccagcact gccggctacc ccaccagcca ccccgcctgc agcctctccc agtgctctga
     1741 agggggtgtc ccaggatctg ctggagcgga tccgagccaa ggaggcacag aagcagctgg
     1801 cacagatgac gcggtgcccg gagcaggagc agcggctgca gcgcttagaa cggctgcctg
     1861 agctggcccg cgtgctgcgg agcgtctttg tgtccgaacg caagcctgcg ctcagcatgg
     1921 aggtggcctg tgccaggatg gtgggcagct gttgtactat catgagccct ggggaaatgg
     1981 agaagcacct gctgctcctc tccgagctgc tgccggactg gctcagcctc caccgcatcc
     2041 gcaccgacac ctacgtcaag ctggacaagg ccgcggacct cgcccacatc actgcacgcc
     2101 tggcccacca gacacgtgct gaggaggggc tgggtgcagg aagcgctggg ggatccatgg
     2161 cacctaagaa aaagcggaaa gtgggcggaa gtggttatcc gtacgacgta ccagactacg
     2221 catgagaatt cgatccgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc
     2281 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     2341 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     2401 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     2461 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     2521 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     2581 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     2641 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     2701 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagcaa ttcaccatga
     2761 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     2821 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     2881 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     2941 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     3001 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     3061 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     3121 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     3181 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     3241 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     3301 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccgaaggta
     3361 gaggttctct cctcacttgt ggtgatgttg aagaaaaccc tggtccaatg tctagactgg
     3421 acaagagcaa agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc
     3481 tgacgacaag gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg
     3541 tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata
     3601 cccacttctg ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt
     3661 cataccgctg tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc
     3721 caacagagaa acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct
     3781 tctccctgga gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg
     3841 tattggagga acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt
     3901 ctatgccccc acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg
     3961 ccttcctttt cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa
     4021 gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc
     4081 ttgacgactt tgaccttgat atgctgcctg ctgacgctct tgacgatttt gaccttgaca
     4141 tgctccccgg gtaactaagt aaggatcgat ccaagatatc gtattcttaa ctatgttgct
     4201 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4261 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4321 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4381 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4441 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4501 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4561 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4621 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4681 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtttcgcct
     4741 cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct gtagatctta
     4801 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4861 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4921 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4981 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5041 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5101 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     5161 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5221 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     5281 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5401 gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata
     5461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7321 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7381 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7441 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7501 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     7561 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     7621 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     7681 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     7741 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     7801 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7861 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7921 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7981 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8041 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8101 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8161 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8221 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8281 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8341 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8401 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8461 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8521 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8581 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8641 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8701 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8761 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8821 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8881 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8941 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9001 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9061 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9121 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9181 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9241 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggac
//
