LOCUS       sequence-380214-        9151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1 (SDV40 NLS , C-terminal HA )
            w/ mutations stopping S phase degradation and in MCM binding domain 
            (Delta-499-546), expressed from all-in-one pCW vector (TetOn + 
            rtTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pCW-ND-Cdt1-Delta-499-546-HA-Puro
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2019..2039
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2052..2078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2093..2603
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2522..2540
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2613..3209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2613..2632)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3109..3129
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3210..3263
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3264..3881,3882..3887,3888..3923,3924..3926,3927..3962,
                     3963..3965,3966..4001,4002..4010)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4050..4070)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4468..4479)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4684..4917
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4989..5110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5026..5045)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5080..5099
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5150..5285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5285..5356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5357..5425,5426..6217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5575..5594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6388..6976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6877..6896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7130..7147
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7264..7285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7300..7305,7306..7323,7324..7330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7338..7354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7343..7365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7362..7378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7362..7378
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7397..7417
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7399..7417
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7445..7671
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7672..7852
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7899..8024
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8517..8750
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8935..8979
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gatagctccg ccaaagctcg cttgtcggac tccctctccg gctagaccgg
      601 cacttcgcgc ccccgcctcc gctacttcag gtagccgaaa gagggcgagg ccaccagcag
      661 cccccggacg cgatcaggcg aggccacctg ctcgagcggc tgctcgcctc tcagtagatg
      721 aggttagcag ccccagtacc cccgaggccc cagacatccc agcctgccct tctccgggcc
      781 agaagataaa gaaatccacc ccggcagcag gtcagccgcc ccacctgaca tccgcgcagg
      841 accaggacac catctctgag cttgcgtcat gcctgcaacg ggcccgggag ctgggggcaa
      901 gagtccgggc gctgaaggcc agtgcccagg atgctgggga gtcctgcacc ccagaggccg
      961 agggccgccc tgaggagcca tgtggcgaga aggcgcccgc ctaccagcgc ttccatgccc
     1021 tggcccagcc cggcctgccg ggactcgtgc tgccctacaa gtaccaggtg ctggcggaga
     1081 tgttccgcag catggacacc atcgtgggca tgctccacaa ccgctccgag acgcccacct
     1141 ttgccaaggt ccagcggggc gtccaggaca tgatgcgtag gcgttttgag gagcgcaatg
     1201 ttggccagat caaaaccgtg tacccggcct cctaccgctt ccgccaggag cgcagtgtcc
     1261 ccaccttcaa ggatggcacc aggaggtcag attaccagct caccatcgag ccactgctgg
     1321 agcaggaggc tgacggagca gccccccagc tcacggcctc gcgcctcctg cagcgacggc
     1381 agatcttcag ccagaagctg gtggagcatg tcaaggagca ccacaaggcc ttcctggcct
     1441 ccctgagccc cgccatggtg gtgccggagg accagctgac ccgctggcac ccgcgcttca
     1501 acgtggatga agtacccgac atcgagccgg ccgcgctgcc ccagccaccc gccacggaga
     1561 agctcaccac tgctcaggag gtgctggccc gggcccgcaa cctgatttca cccaggatgg
     1621 agaaggcctt gagtcaattg gccctgcgct ctgctgcgcc cagcagcccc gggtctccca
     1681 ggccagcact gccggctacc ccaccagcca ccccgcctgc agcctctccc agtgctctga
     1741 agggggtgtc ccaggatctg ctggagcgga tccgagccaa ggaggcacag aagcagctgg
     1801 cacagatgac gcggtgcccg gagcaggagc agcggctgca gcgcttagaa cggctgcctg
     1861 agctggcccg cgtgctgcgg agcgtctttg tgtccgaacg caagcctgcg ctcagcatgg
     1921 aggtggcctg tgccaggatg gtgggcagct gttgtactat catgagccct ggggaaatgg
     1981 agaagcacgg tgcaggaagc gctgggggat ccatggcacc taagaaaaag cggaaagtgg
     2041 gcggaagtgg ttatccgtac gacgtaccag actacgcatg agaattcgat ccggggttgg
     2101 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     2161 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     2221 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     2281 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     2341 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     2401 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     2461 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     2521 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     2581 gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc acggtgcgcc
     2641 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     2701 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     2761 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     2821 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     2881 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     2941 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     3001 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3061 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3121 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3181 cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc acttgtggtg
     3241 atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag
     3301 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     3361 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     3421 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     3481 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     3541 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     3601 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     3661 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     3721 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     3781 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     3841 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     3901 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     3961 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     4021 atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4081 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4141 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4201 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     4261 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4321 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4381 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     4441 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4501 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4561 atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt
     4621 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     4681 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4741 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4801 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4861 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4921 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4981 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5101 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5221 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5281 aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7081 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7141 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7201 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7261 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7321 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7381 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     7441 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     7501 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7561 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7621 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7681 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7741 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7801 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7861 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7921 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7981 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8041 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8101 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     8161 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8221 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8281 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8341 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     8401 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8461 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8521 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8581 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8641 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8701 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8761 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8821 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8881 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8941 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9001 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9061 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9121 tttcagaccc acctcccaac cccgagggga c
//
