LOCUS       sequence-380219-        3102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to produce ISH probe for mMyh13 gene with UTR sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Tanji M, Wada K, Sakamoto K, Ono Y, Inui M
  TITLE     Digoxigenin-labeled RNA probes for untranslated regions enable the 
            isoform-specific gene expression analysis of myosin heavy chains in 
            whole-mount in situ hybridization.
  JOURNAL   Dev Growth Differ. 2022 Dec 14. doi: 10.1111/dgd.12832.
  PUBMED    36517457
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Growth 
            Differ. 2022 Dec 14. doi: 10.1111/dgd.12832."
COMMENT     Sequence Label: pGEMT-mMyh13UTR
FEATURES             Location/Qualifiers
     source          1..3102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     108..127
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     361..378
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    495..516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(531..536,537..554,555..561)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    569..585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     574..596
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     593..609
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     593..609
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        627..645
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     627..644
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(869..888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(870..888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(895..912)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(895..911)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(904..926)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1052..1507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1139..1158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1349..1370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1585..1689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1690..1758,1759..2550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1908..1927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2721..3102,1..207)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
       61 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      361 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      481 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      541 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      601 agctatgacc atgattacgc caagctattt aggtgacact atagaatact caagctatgc
      661 atccaacgcg ttgggagctc tcccatatgg tcgacctgca ggcggccgcg aattcactag
      721 tgattgctga atctgcagga gggtttattt ttctcaagtc tccctttgct ctcccggagg
      781 tggcccatgg caacgtgcct ctggagagcc aatcgaattc ccgcggccgc catggcggcc
      841 gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag tcgtattaca attcactggc
      901 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      961 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1021 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
     1081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1261 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1321 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1381 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1441 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1501 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatca
     1561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1921 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     2041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     2101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     2161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     2221 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc ta
//
