LOCUS       sequence-38022-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Jirawatnotai S, Hu Y, Michowski W, Elias JE, Becks L, Bienvenu F, 
            Zagozdzon A, Goswami T, Wang YE, Clark AB, Kunkel TA, van Harn T, 
            Xia B, Correll M, Quackenbush J, Livingston DM, Gygi SP, Sicinski P
  TITLE     A function for cyclin D1 in DNA repair uncovered by protein 
            interactome analyses in human cancers.
  JOURNAL   Nature. 2011 Jun 8;474(7350):230-4. doi: 10.1038/nature10155.
  PUBMED    21654808
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-8- 8"; volume: "474"; issue: "7350"; pages:
            "230-4"
COMMENT     Sequence Label: Cyclin D1 shRNA-B
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(270..290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        275..604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      455..590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(614..633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccggcca cagatgtgaa gttcatttct cgagaaatga acttcacatc tgtggttttt
      241 gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
      301 cccaggcttc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      601 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      661 cnccagggcc gtacgcaccc tcgccgccgc gttcgccgac tacnccgcca cgcgccacac
      721 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      781 cgtcgggctc gacatcggcn aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      841 gacca
//
