LOCUS       sequence-380221-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and 
            C-terminal mCherry tag) with mutations which abroggate its S phase 
            degradation, expressed from all-in-one pCW vector (TetOn + rtTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pCW-ND-Cdt1-mCherry-Puro
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2163..2183
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2196..2906
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2341..2359)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2609..2628
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2918..3428
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3347..3365
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3438..4034
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3438..3457)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3934..3954
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4035..4088
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4089..4706,4707..4712,4713..4748,4749..4751,4752..4787,
                     4788..4790,4791..4826,4827..4835)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4875..4895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5293..5304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5509..5742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5814..5935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5851..5870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5905..5924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5975..6110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6110..6181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6182..6250,6251..7042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6400..6419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7213..7801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7702..7721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7955..7972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8089..8110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8125..8130,8131..8148,8149..8155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8163..8179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8168..8190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8187..8203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8187..8203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8222..8242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8224..8242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8270..8496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8497..8677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8724..8849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9342..9575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9760..9804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat gatagctccg ccaaagctcg cttgtcggac tccctctccg gctagaccgg
      601 cacttcgcgc ccccgcctcc gctacttcag gtagccgaaa gagggcgagg ccaccagcag
      661 cccccggacg cgatcaggcg aggccacctg ctcgagcggc tgctcgcctc tcagtagatg
      721 aggttagcag ccccagtacc cccgaggccc cagacatccc agcctgccct tctccgggcc
      781 agaagataaa gaaatccacc ccggcagcag gtcagccgcc ccacctgaca tccgcgcagg
      841 accaggacac catctctgag cttgcgtcat gcctgcaacg ggcccgggag ctgggggcaa
      901 gagtccgggc gctgaaggcc agtgcccagg atgctgggga gtcctgcacc ccagaggccg
      961 agggccgccc tgaggagcca tgtggcgaga aggcgcccgc ctaccagcgc ttccatgccc
     1021 tggcccagcc cggcctgccg ggactcgtgc tgccctacaa gtaccaggtg ctggcggaga
     1081 tgttccgcag catggacacc atcgtgggca tgctccacaa ccgctccgag acgcccacct
     1141 ttgccaaggt ccagcggggc gtccaggaca tgatgcgtag gcgttttgag gagcgcaatg
     1201 ttggccagat caaaaccgtg tacccggcct cctaccgctt ccgccaggag cgcagtgtcc
     1261 ccaccttcaa ggatggcacc aggaggtcag attaccagct caccatcgag ccactgctgg
     1321 agcaggaggc tgacggagca gccccccagc tcacggcctc gcgcctcctg cagcgacggc
     1381 agatcttcag ccagaagctg gtggagcatg tcaaggagca ccacaaggcc ttcctggcct
     1441 ccctgagccc cgccatggtg gtgccggagg accagctgac ccgctggcac ccgcgcttca
     1501 acgtggatga agtacccgac atcgagccgg ccgcgctgcc ccagccaccc gccacggaga
     1561 agctcaccac tgctcaggag gtgctggccc gggcccgcaa cctgatttca cccaggatgg
     1621 agaaggcctt gagtcaattg gccctgcgct ctgctgcgcc cagcagcccc gggtctccca
     1681 ggccagcact gccggctacc ccaccagcca ccccgcctgc agcctctccc agtgctctga
     1741 agggggtgtc ccaggatctg ctggagcgga tccgagccaa ggaggcacag aagcagctgg
     1801 cacagatgac gcggtgcccg gagcaggagc agcggctgca gcgcttagaa cggctgcctg
     1861 agctggcccg cgtgctgcgg agcgtctttg tgtccgaacg caagcctgcg ctcagcatgg
     1921 aggtggcctg tgccaggatg gtgggcagct gttgtactat catgagccct ggggaaatgg
     1981 agaagcacct gctgctcctc tccgagctgc tgccggactg gctcagcctc caccgcatcc
     2041 gcaccgacac ctacgtcaag ctggacaagg ccgcggacct cgcccacatc actgcacgcc
     2101 tggcccacca gacacgtgct gaggaggggc tgggtgcagg aagcgctggg ggatccatgg
     2161 cacctaagaa aaagcggaaa gtgggcggaa gtggtatggt gagcaagggc gaggaggata
     2221 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2281 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2341 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2401 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2461 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2521 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2581 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2641 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     2701 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     2761 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     2821 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     2881 gcggcatgga cgagctgtac aagtgagaat tcgatccggg gttggggttg cgccttttcc
     2941 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     3001 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     3061 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     3121 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     3181 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     3241 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     3301 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     3361 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     3421 ctccccagca attcaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     3481 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     3541 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     3601 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     3661 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     3721 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     3781 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     3841 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     3901 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     3961 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     4021 gcaagcccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt gaagaaaacc
     4081 ctggtccaat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     4141 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     4201 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     4261 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     4321 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     4381 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     4441 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     4501 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     4561 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     4621 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     4681 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     4741 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     4801 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga tccaagatat
     4861 cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     4921 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     4981 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5041 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5101 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5161 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     5221 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5281 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5341 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5401 gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt
     5461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5521 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5941 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat
     6121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8041 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8101 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8161 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8221 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     8281 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     8341 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     8401 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     8461 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     8521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8641 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8701 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8761 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8821 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8881 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8941 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9001 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9061 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9121 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     9181 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9241 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9301 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9361 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9421 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9481 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9541 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9601 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9661 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9721 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9781 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9841 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9901 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     9961 ccaaccccga ggggac
//
