LOCUS       sequence-380222-        9991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), 
            expressed from all-in-one pCW vector (TetOn + rtTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pCW-Cdt1-mCherry-Puro
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      543..552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             636..908
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             2211..2921
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2356..2374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2624..2643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2933..3443
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3362..3380
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3453..4049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3453..3472)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3949..3969
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4050..4103
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4104..4721,4722..4727,4728..4763,4764..4766,4767..4802,
                     4803..4805,4806..4841,4842..4850)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4890..4910)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5308..5319)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5524..5757
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5829..5950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5866..5885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5920..5939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5990..6125
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6125..6196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6197..6265,6266..7057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6415..6434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7228..7816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7717..7736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7970..7987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8104..8125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8140..8145,8146..8163,8164..8170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8178..8194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8183..8205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8202..8218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8202..8218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8237..8257
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8239..8257
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8285..8511
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8512..8692
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8739..8864
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9357..9590
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9775..9819
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcgccaccat ggagcagcgc cgcgtcaccg acttcttcgc gcgccgccgc cccgggcccc
      601 cccgcatcgc gccgcccaag ctggcctgcc gcacccccag ccccgccagg cccgcactcc
      661 gcgccccggc ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc gccgcccccg
      721 gacgcgacca ggccaggcca ccggcccgca ggagactgcg gctgtcggtg gacgaggttt
      781 ccagccccag tacccccgag gccccagaca tcccagcctg cccttctccg ggccagaaga
      841 taaagaaatc caccccggca gcaggtcagc cgccccacct gacatccgcg caggaccagg
      901 acaccatctc tgagcttgcg tcatgcctgc aacgggcccg ggagctgggg gcaagagtcc
      961 gggcgctgaa ggccagtgcc caggatgctg gggagtcctg caccccagag gccgagggcc
     1021 gccctgagga gccatgtggc gagaaggcgc ccgcctacca gcgcttccat gccctggccc
     1081 agcccggcct gccgggactc gtgctgccct acaagtacca ggtgctggcg gagatgttcc
     1141 gcagcatgga caccatcgtg ggcatgctcc acaaccgctc cgagacgccc acctttgcca
     1201 aggtccagcg gggcgtccag gacatgatgc gtaggcgttt tgaggagcgc aatgttggcc
     1261 agatcaaaac cgtgtacccg gcctcctacc gcttccgcca ggagcgcagt gtccccacct
     1321 tcaaggatgg caccaggagg tcagattacc agctcaccat cgagccactg ctggagcagg
     1381 aggctgacgg agcagccccc cagctcacgg cctcgcgcct cctgcagcga cggcagatct
     1441 tcagccagaa gctggtggag catgtcaagg agcaccacaa ggccttcctg gcctccctga
     1501 gccccgccat ggtggtgccg gaggaccagc tgacccgctg gcacccgcgc ttcaacgtgg
     1561 atgaagtacc cgacatcgag ccggccgcgc tgccccagcc acccgccacg gagaagctca
     1621 ccactgctca ggaggtgctg gcccgggccc gcaacctgat ttcacccagg atggagaagg
     1681 ccttgagtca attggccctg cgctctgctg cgcccagcag ccccgggtct cccaggccag
     1741 cactgccggc taccccacca gccaccccgc ctgcagcctc tcccagtgct ctgaaggggg
     1801 tgtcccagga tctgctggag cggatccgag ccaaggaggc acagaagcag ctggcacaga
     1861 tgacgcggtg cccggagcag gagcagcggc tgcagcgctt agaacggctg cctgagctgg
     1921 cccgcgtgct gcggagcgtc tttgtgtccg aacgcaagcc tgcgctcagc atggaggtgg
     1981 cctgtgccag gatggtgggc agctgttgta ctatcatgag ccctggggaa atggagaagc
     2041 acctgctgct cctctccgag ctgctgccgg actggctcag cctccaccgc atccgcaccg
     2101 acacctacgt caagctggac aaggccgcgg acctcgccca catcactgca cgcctggccc
     2161 accagacacg tgctgaggag gggctgggtg caggaagcgc tgggggatcc atggtgagca
     2221 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     2281 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2341 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     2401 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     2461 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2521 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     2581 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     2641 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     2701 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2761 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2821 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2881 gccgccactc caccggcggc atggacgagc tgtacaagtg agaattcgat ccggggttgg
     2941 ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     3001 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     3061 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     3121 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     3181 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     3241 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     3301 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     3361 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     3421 gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc acggtgcgcc
     3481 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     3541 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     3601 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     3661 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     3721 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     3781 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     3841 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3901 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3961 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     4021 cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc acttgtggtg
     4081 atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc ataaacggag
     4141 ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     4201 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     4261 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     4321 agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac
     4381 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     4441 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     4501 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     4561 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     4621 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     4681 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg
     4741 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     4801 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     4861 atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4921 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4981 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5041 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5101 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5161 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5221 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5281 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5341 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5401 atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta gaggtacctt
     5461 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5521 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5581 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5641 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5701 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5761 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5821 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5881 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5941 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6001 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6061 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6121 aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     6181 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     6241 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     6301 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     6361 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     6421 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     6481 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     6541 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6601 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6661 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6721 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6781 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6841 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6901 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6961 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     7021 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     7081 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     7141 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7201 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7261 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7321 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7381 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7441 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7501 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7561 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7621 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7681 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7741 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7801 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7861 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7921 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7981 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     8041 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     8101 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     8161 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     8221 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     8281 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     8341 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     8401 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     8461 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     8521 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8581 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8641 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     8701 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     8761 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     8821 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     8881 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     8941 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     9001 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     9061 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     9121 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     9181 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     9241 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     9301 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     9361 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     9421 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     9481 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     9541 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     9601 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     9661 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     9721 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     9781 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     9841 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     9901 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     9961 tttcagaccc acctcccaac cccgagggga c
//
