LOCUS       sequence-380224-       11430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SpdCas9 fused to N-terminal part of mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Lungu C, Pinter S, Broche J, Rathert P, Jeltsch A
  TITLE     Modular fluorescence complementation sensors for live cell detection
            of epigenetic signals at endogenous genomic sites.
  JOURNAL   Nat Commun. 2017 Sep 21;8(1):649. doi: 10.1038/s41467-017-00457-z.
  PUBMED    28935858
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-21"; volume: "8(1):649. doi"; pages: " 
            10.1038/s41467-017-00457-z"
COMMENT     Sequence Label: pHAGE-TO-Sp dCas9-mVenusN
FEATURES             Location/Qualifiers
     source          1..11430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             168..801
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    848..973
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1470..1703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1888..1932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2225..2342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2466..2769
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2770..2973
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2924..2944
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2975..2993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2996..3014
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3024..3048
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3141..3150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3153..3173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3192..3215,3216..3218,3219..5702,5703..5708,5709..5774,
                     5775..5777,5778..7292)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3192..3215,3216..3218,3219..5705,5706..5708,
                     5709..7292)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3192..7292
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7311..7331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7344..7346,7347..7349,7350..7862)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(7389..7410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7650..7669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    7987..8575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8040..8060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8458..8469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8650..8883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9002..9020)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9088..9192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9193..9261,9262..10053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9411..9430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10224..10812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10713..10732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10966..10983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11223..11244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11259..11264,11265..11282,11283..11289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11297..11313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11302..11324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11321..11337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11321..11337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11387..11406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
       61 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
      121 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
      181 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
      241 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
      301 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
      361 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
      421 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
      481 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
      541 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
      601 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      781 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      841 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      901 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
      961 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1021 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1081 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1141 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1201 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1261 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
     1321 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1381 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1441 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1501 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1561 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1621 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1681 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1741 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1801 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     1861 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     1921 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     1981 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2041 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2101 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2161 attcgattag tgaacggatc tcgacggtat cgccgaattg acaaatggca gtattcatcc
     2221 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2281 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2341 ttcgggttta ttacagggac agcagagatc cagtttggac tagcgatgct agctgatgcg
     2401 ggccctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     2461 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2521 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2581 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2641 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2701 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2761 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     2821 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     2881 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     2941 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
     3001 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3061 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct accggttaag
     3121 gtgaacgcgt gcaggctggc gccaccatgg cccctaaaaa gaaacgcaag gtcgaattcg
     3181 gaagcggaag cgacaagaag tactccattg ggctcgctat cggtaccaac agcgtcggct
     3241 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
     3301 ccgatcgcca cagcataaag aagaacctca ttggagccct cctgttcgac tccggggaga
     3361 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
     3421 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
     3481 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
     3541 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
     3601 tgaggaagaa gctggtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
     3661 tggcgcacat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
     3721 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgagg
     3781 agaacccgat caacgcatcc ggcgttgacg ccaaagcaat cctgagcgct aggctgtcca
     3841 aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt
     3901 ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc
     3961 tggccgaaga tgccaagctg caactgagca aagacaccta cgatgatgat ctcgacaatc
     4021 tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag aacctgtcag
     4081 acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa gctccgctga
     4141 gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg ctgaaggccc
     4201 ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag tctaaaaatg
     4261 gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa tttattaagc
     4321 ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagctgaac agagaagatc
     4381 tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt cacctgggcg
     4441 aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa gataacaggg
     4501 aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc ctcgctcggg
     4561 gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact ccctggaact
     4621 tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg atgactaact
     4681 ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact
     4741 tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg agaaagccag
     4801 cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag acgaaccgga
     4861 aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt ttcgactctg
     4921 ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat cacgatctcc
     4981 tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac attcttgagg
     5041 acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa cgcttgaaaa
     5101 cttacgctca tctcttcgac gacaaagtca tgaaacagct caagagacgc cgatatacag
     5161 gatgggggcg gctgtcaaga aaactgatca atggcatccg agacaagcag agtggaaaga
     5221 caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg cagttgatcc
     5281 atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct ggccaggggg
     5341 acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa aagggaatac
     5401 tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat aagcccgaga
     5461 atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag aagaacagta
     5521 gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa atccttaagg
     5581 aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac tacctgcaga
     5641 acggcaggga catgtacgtg gatcaggaac tggacatcaa ccggttgtcc gactacgacg
     5701 tggatgctat cgtgccccaa agctttctca aagatgattc tattgataat aaagtgttga
     5761 caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga
     5821 aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa cggaagttcg
     5881 ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca
     5941 aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt ctcgattcac
     6001 gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa gttattactc
     6061 tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag gtgagagaga
     6121 tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc actgcactta
     6181 tcaaaaaata tcccaagctg gaatctgaat ttgtttacgg agactataaa gtgtacgatg
     6241 ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct aagtacttct
     6301 tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat ggagagattc
     6361 ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg gacaagggta
     6421 gggatttcgc gacagtccgc aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga
     6481 ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg aacagcgaca
     6541 agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc gattctccta
     6601 cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct aaaaaactca
     6661 aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atccagcttc gagaaaaacc
     6721 ccatcgactt tctcgaagcg aaaggatata aagaggtcaa aaaagacctc atcattaagc
     6781 tgcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc gctagtgcgg
     6841 gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat ttcttgtatc
     6901 tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag aagcagctgt
     6961 tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc gagttctcca
     7021 aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac aataagcaca
     7081 gggataagcc catcagggag caggcagaaa acattatcca cttgtttact ctgaccaact
     7141 tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag cggtacacct
     7201 ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg ctctatgaaa
     7261 caagaatcga cctctctcag ctcggtggag acggctctac tagtggctct cccaagaaga
     7321 agaggaaggt aggatccgga agtatggtga gcaagggcga ggagctgttc accggggtgg
     7381 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     7441 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctcatctgc accaccggca
     7501 agctgcccgt gccctggccc accctcgtga ccaccctcgg ctacggcctg cagtgcttcg
     7561 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     7621 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     7681 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     7741 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     7801 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     7861 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     7921 ccgtgctgct gcccgacaac cactacctga gctaccagtc caagctgagc aaagactaat
     7981 ctagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     8581 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     8641 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8701 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8761 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8821 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8881 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8941 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     9001 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     9061 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     9181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9421 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10981 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    11041 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    11101 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    11161 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    11221 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11281 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    11341 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11401 ctcatcaatg tatcttatca tgtctggatc
//
