LOCUS       sequence-380234-       10719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses bicistronic P2A construct of C-CRL4Cdt2 
            reporter(hCdt1(1-100)Delta-Cy-mCherry-) and APC/C reporter 
            (hGeminin(1-110)) with blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: 
            pLV-hCdt1(1-100)Delta-Cy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Bla
            st
FEATURES             Location/Qualifiers
     source          1..10719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    207..332
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    829..1062
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1247..1291
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1554..1669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        1747..1958
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1959..2897
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2854..2874
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3234..3941
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3379..3397)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3647..3666
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3951..4007
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4017..4019,4020..4022,4023..4733)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4062..4083)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4323..4342)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4670..4691
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4764..5093
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    5210..5759
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5354..5371)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5581..5600
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6214..6802
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6267..6287)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6685..6696)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7009..7642
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7770..7786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7770..7786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7783..7805)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7794..7810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7818..7824,7825..7842,7843..7848))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7863..7884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8001..8018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8172..8760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8252..8271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8931..9722,9723..9791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9554..9573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9792..9896)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9944..10078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9981..10000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10035..10054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10246..10719,1..160)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
       61 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
      121 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
      181 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
      241 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
      301 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
      361 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
      421 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
      481 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
      541 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
      601 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
      661 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
      721 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
      781 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
      841 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
      901 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
      961 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1021 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1081 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1141 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1201 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     1261 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     1321 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     1381 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     1441 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     1501 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gcctttaaaa
     1561 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1621 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1681 acagggacag cagagatcca gtttatcgat gaggcccttt cgtcttcact cgaggtgccc
     1741 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1801 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1861 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     1921 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     1981 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     2041 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     2101 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     2161 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     2221 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     2281 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     2341 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     2401 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     2461 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     2521 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     2581 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     2641 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     2701 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     2761 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     2821 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     2881 tttttcttcc atttcaggtg tcgtgaggat ccaccatgga acaacggagg gttactgact
     2941 ttttcgcgag gagacgaccc ggccccccga ggatcgcacc cccgaaactc gcttgtagaa
     3001 ccccatctcc cgccaggcca gcgcttcggg cgccagcaag tgctacttct ggtagtcgaa
     3061 aacgggcgag accacctgct gctccgggga gagaccaggc aagacccccg gcgagggcag
     3121 ctgccagatt gagtgttgac gaagtatcct ctccatctac tccagaagca cctgacattc
     3181 cggcttgccc ctctccggga cagaaaataa agaaaggtgc aggaagcgct gggatggtga
     3241 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     3301 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3361 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     3421 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     3481 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3541 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     3601 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     3661 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     3721 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     3781 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     3841 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     3901 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggcagcggc gccaccaact
     3961 tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggcccccgc gaattcatgg
     4021 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4081 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4141 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4201 tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     4261 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4321 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4381 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4441 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     4501 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     4561 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4621 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4681 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggatatc
     4741 catcacactg gcggccgctc gagatgaatc ccagtatgaa gcagaaacaa gaagaaatca
     4801 aagagaatat aaagaatagt tctgtcccaa gaagaactct gaagatgatt cagccttctg
     4861 catctggatc tcttgttgga agagaaaatg agctgtccgc aggcttgtcc aaaaggaaac
     4921 atcggaatga ccacttaaca tctacaactt ccagccctgg ggttattgtc ccagaatcta
     4981 gtgaaaataa aaatcttgga ggagtcaccc aggagtcatt tgatcttatg attaaagaaa
     5041 atccatcctc tcagtattgg aaggaagtgg cagaaaaacg gagaaaggcg ctgtaagaat
     5101 tcctcgaggg cggccgctct agagtcgacg ggccgcggta acaattgtta actaacttaa
     5161 gctagcaacg gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc
     5221 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5281 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5341 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5401 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5461 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5521 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5581 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5641 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5701 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
     5761 catggtcatg aaaacattta acatttctca acaagatcta gaattagtag aagtagcgac
     5821 agagaagatt acaatgcttt atgaggataa taaacatcat gtgggagcgg caattcgtac
     5881 gaaaacagga gaaatcattt cggcagtaca tattgaagcg tatataggac gagtaactgt
     5941 ttgtgcagaa gccattgcga ttggtagtgc agtttcgaat ggacaaaagg attttgacac
     6001 gattgtagct gttagacacc cttattctga cgaagtagat agaagtattc gagtggtaag
     6061 tccttgtggt atgtgtaggg agttgatttc agactatgca ccagattgtt ttgtgttaat
     6121 agaaatgaat ggcaagttag tcaaaactac gattgaagaa ctcattccac tcaaatatac
     6181 ccgaaattaa cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
     6241 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6301 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6361 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6421 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6481 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6541 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6601 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     6661 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6721 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6781 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     6841 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6901 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     6961 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa
     7021 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     7081 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     7141 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     7201 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     7261 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     7321 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     7381 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     7441 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7501 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7561 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7621 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7681 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg
     7741 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7801 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7861 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7921 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7981 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9901 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     9961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10081 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10141 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10201 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
    10261 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
    10321 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
    10381 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
    10441 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
    10501 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
    10561 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
    10621 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
    10681 ctgctttttg cctgtactgg gtctctctgg ttagaccag
//
