LOCUS       sequence-380237-        9839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 
            of Loop 1 of LwaCas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Yang J, Song Y, Deng X, Vanegas JA, You Z, Zhang Y, Weng Z, Avery L,
            Dieckhaus KD, Peddi A, Gao Y, Zhang Y, Gao X
  TITLE     Engineered LwaCas13a with enhanced collateral activity for nucleic 
            acid detection.
  JOURNAL   Nat Chem Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 
            10.1038/s41589-022-01135-y.
  PUBMED    36138140
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 
            10.1038/s41589-022-01135-y."
COMMENT     Sequence Label: RBD#3L
FEATURES             Location/Qualifiers
     source          1..9839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     969..988
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1008..1085)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1291..1310
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1394..1413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1394..1412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1413..1437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1452..1474
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1493..1510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1520..1537
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(1550..1573,1574..1609,1610..1633)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             1634..1927
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(5733..5751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5747..5794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5969..5974,5975..5991,5992..5997))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6024..6042)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6110..6214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6215..6283,6284..7075)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6433..6452)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7246..7834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7735..7754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7988..8005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8020..8159
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8085..8104)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8245..8267
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8261..8452)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9764..9839,1..1007))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
       61 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      121 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
      181 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      241 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      301 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      361 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      421 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      481 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
      541 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      601 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      661 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      721 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      781 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
      841 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
      901 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
      961 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     1021 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     1081 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1141 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     1201 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1261 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1321 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
     1381 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
     1441 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
     1501 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggctagct ggagccatcc
     1561 gcagtttgaa aaaggtggtg gtagcggtgg tggttcaggt ggtagtgcat ggtcacaccc
     1621 tcagtttgag aaaatgtcgg actcagaagt caatcaagaa gctaagccag aggtcaagcc
     1681 agaagtcaag cctgagactc acatcaattt aaaggtgtcc gatggatctt cagagatctt
     1741 cttcaagatc aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca
     1801 gggtaaggaa atggactcct taagattctt gtacgacggt attagaatcc aagctgatca
     1861 gacccctgaa gatttggaca tggaggataa cgatattatt gaggctcaca gagaacagat
     1921 tggtggatcc atgaaagtga ccaaggtcga cggcatcagc cacaagaagt acatcgaaga
     1981 gggcaagctc gtgaagtcca ccagcgagga aaaccggacc agcgagagac tgagcgagct
     2041 gctgagcatc cggctggaca tctacatcaa gaaccccgac aacgcctccg aggaagagaa
     2101 ccggatcaga agagagaacc tgaagaagtt ctttagcaac aaggtgctgc acctgaagga
     2161 cagcgtgctg tatctgaaga accggaaaga aaagaacgcc gtgcaggaca agaactatag
     2221 cgaagaggac atcagcgagt acgacctgaa aaacaagaac agcttctccg tgctgaagaa
     2281 gatcctgctg aacgaggacg tgaactctga ggaactggaa atctttcgga aggacgtgga
     2341 agccaagctg aacaagatca acagcctgaa gtacagcttc gaagagaaca aggccaacta
     2401 ccagaagatc aacgagaaca acgtggaaaa agtgggcggc aagagcaagc ggaacatcat
     2461 ctacgactac tacagagaga gcgccaagcg caacgactac atcaacaacg tgcaggaagc
     2521 cttcgacaag ctgtataaga aagaggatat cgagaaactg tttttcctga tcgagaacag
     2581 caagaagcac gagaagtaca agatccgcga gtactatcac aagatcatcg gccggaagaa
     2641 cgacaaagag aacttcgcca agattatcta cgaagagatc cagaacgtga acaacatcaa
     2701 agagctgatt gagaagatcc ccgacatgtc tgagctgaag aaaagccagg tgttctacaa
     2761 gtactacctg gacaaagagg aactgaacga caagaatatt aagtacgcct tctgccactt
     2821 cgtggaaatc gagatgtccc agctgctgaa aaactacgtg tacaagcggc tgagcaacat
     2881 cagcaacgat aagatcaagc ggatcttcga gtaccagaat ctgaaaaagc tgatcgaaaa
     2941 caaactgctg aacaagctgg acacctacgt gcggaactgc ggcaagtaca actactatct
     3001 gcaagtgggc gagatcgcca cctccgactt tatcgcccgg aaccggcaga acgaggcctt
     3061 cctgagaaac atcatcggcg tgtccagcgt ggcctacttc agcctgagga acatcctgga
     3121 aaccgagaac gagaacgata tcaccggccg gatgcggggc aagaccgtga agaacccagg
     3181 tgcccactta actgtgaaaa agatatttgt tggtggcatt aaagaagaca ctgaagaaca
     3241 tcacctaaga gattattttg aacagtatgg aaaaattgaa gtgattgaaa tcatgactga
     3301 ccgaggcagt ggcaagaaaa ggggctttgc ctttgtaacc tttgacgacc atgactccgt
     3361 ggataagatt gtcattcaga aataccatac tgtgaatggc cacaactgtg aagttagaaa
     3421 agccctgtca aagcaagaga tggctagtgc ttcatccagc caaagaggtc gaaacaaggg
     3481 cgaagagaaa tacgtgtccg gcgaggtgga caagatctac aatgagaaca agcagaacga
     3541 agtgaaagaa aatctgaaga tgttctacag ctacgacttc aacatggaca acaagaacga
     3601 gatcgaggac ttcttcgcca acatcgacga ggccatcagc agcatcagac acggcatcgt
     3661 gcacttcaac ctggaactgg aaggcaagga catcttcgcc ttcaagaata tcgcccccag
     3721 cgagatctcc aagaagatgt ttcagaacga aatcaacgaa aagaagctga agctgaaaat
     3781 cttcaagcag ctgaacagcg ccaacgtgtt caactactac gagaaggatg tgatcatcaa
     3841 gtacctgaag aataccaagt tcaacttcgt gaacaaaaac atccccttcg tgcccagctt
     3901 caccaagctg tacaacaaga ttgaggacct gcggaatacc ctgaagtttt tttggagcgt
     3961 gcccaaggac aaagaagaga aggacgccca gatctacctg ctgaagaata tctactacgg
     4021 cgagttcctg aacaagttcg tgaaaaactc caaggtgttc tttaagatca ccaatgaagt
     4081 gatcaagatt aacaagcagc ggaaccagaa aaccggccac tacaagtatc agaagttcga
     4141 gaacatcgag aaaaccgtgc ccgtggaata cctggccatc atccagagca gagagatgat
     4201 caacaaccag gacaaagagg aaaagaatac ctacatcgac tttattcagc agattttcct
     4261 gaagggcttc atcgactacc tgaacaagaa caatctgaag tatatcgaga gcaacaacaa
     4321 caatgacaac aacgacatct tctccaagat caagatcaaa aaggataaca aagagaagta
     4381 cgacaagatc ctgaagaact atgagaagca caatcggaac aaagaaatcc ctcacgagat
     4441 caatgagttc gtgcgcgaga tcaagctggg gaagattctg aagtacaccg agaatctgaa
     4501 catgttttac ctgatcctga agctgctgaa ccacaaagag ctgaccaacc tgaagggcag
     4561 cctggaaaag taccagtccg ccaacaaaga agaaaccttc agcgacgagc tggaactgat
     4621 caacctgctg aacctggaca acaacagagt gaccgaggac ttcgagctgg aagccaacga
     4681 gatcggcaag ttcctggact tcaacgaaaa caaaatcaag gaccggaaag agctgaaaaa
     4741 gttcgacacc aacaagatct atttcgacgg cgagaacatc atcaagcacc gggccttcta
     4801 caatatcaag aaatacggca tgctgaatct gctggaaaag atcgccgata aggccaagta
     4861 taagatcagc ctgaaagaac tgaaagagta cagcaacaag aagaatgaga ttgaaaagaa
     4921 ctacaccatg cagcagaacc tgcaccggaa gtacgccaga cccaagaagg acgaaaagtt
     4981 caacgacgag gactacaaag agtatgagaa ggccatcggc aacatccaga agtacaccca
     5041 cctgaagaac aaggtggaat tcaatgagct gaacctgctg cagggcctgc tgctgaagat
     5101 cctgcaccgg ctcgtgggct acaccagcat ctgggagcgg gacctgagat tccggctgaa
     5161 gggcgagttt cccgagaacc actacatcga ggaaattttc aatttcgaca actccaagaa
     5221 tgtgaagtac aaaagcggcc agatcgtgga aaagtatatc aacttctaca aagaactgta
     5281 caaggacaat gtggaaaagc ggagcatcta ctccgacaag aaagtgaaga aactgaagca
     5341 ggaaaaaaag gacctgtaca tccggaacta cattgcccac ttcaactaca tcccccacgc
     5401 cgagattagc ctgctggaag tgctggaaaa cctgcggaag ctgctgtcct acgaccggaa
     5461 gctgaagaac gccatcatga agtccatcgt ggacattctg aaagaatacg gcttcgtggc
     5521 caccttcaag atcggcgctg acaagaagat cgaaatccag accctggaat cagagaagat
     5581 cgtgcacctg aagaatctga agaaaaagaa actgatgacc gaccggaaca gcgaggaact
     5641 gtgcgaactc gtgaaagtca tgttcgagta caaggccctg gaataagcgg ccgcactcga
     5701 ggcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     5761 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     5821 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
     5881 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
     5941 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
     6001 tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6061 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6181 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6241 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6301 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6361 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6421 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     6481 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6541 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6601 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6661 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6721 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     6781 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6841 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6901 tgctgataaa tctggagccg gtgagcgtgg atctcgcggt atcattgcag cactggggcc
     6961 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7021 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7381 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     8041 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     8101 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     8161 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8221 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8281 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     8341 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     8401 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     8461 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     8521 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     8581 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     8641 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     8701 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     8761 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     8821 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     8881 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     8941 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     9001 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     9061 atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc
     9121 gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc
     9181 gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga
     9241 agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac
     9301 ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat
     9361 cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc
     9421 gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     9481 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     9541 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     9601 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     9661 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     9721 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     9781 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcg
//
