LOCUS       sequence-380240-        9857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hnRNP C RRM RNA binding domain inserted in between N415 and N416 of 
            Loop 1 of LwaCas13a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Yang J, Song Y, Deng X, Vanegas JA, You Z, Zhang Y, Weng Z, Avery L,
            Dieckhaus KD, Peddi A, Gao Y, Zhang Y, Gao X
  TITLE     Engineered LwaCas13a with enhanced collateral activity for nucleic 
            acid detection.
  JOURNAL   Nat Chem Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 
            10.1038/s41589-022-01135-y.
  PUBMED    36138140
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 Sep 22. pii: 10.1038/s41589-022-01135-y. doi: 
            10.1038/s41589-022-01135-y."
COMMENT     Sequence Label: RBD#4L
FEATURES             Location/Qualifiers
     source          1..9857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     966..985
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1005..1082)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1288..1307
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1391..1410
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1391..1409
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1410..1434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1449..1471
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1490..1507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1517..1534
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(1547..1570,1571..1606,1607..1630)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             1631..1924
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(5748..5766)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5762..5809
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(5984..5989,5990..6006,6007..6012))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(6039..6057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6125..6229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6230..6298,6299..7090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6448..6467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7261..7849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7750..7769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8003..8020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8035..8174
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8100..8119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8260..8282
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8276..8467)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9779..9857,1..1004))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
       61 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
      121 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
      181 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
      241 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
      301 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
      361 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
      421 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
      481 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
      541 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
      601 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
      661 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
      721 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
      781 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
      841 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
      901 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
      961 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     1021 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     1081 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     1141 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
     1201 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     1261 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     1321 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat
     1381 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta
     1441 gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
     1501 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gctagctgga gccatccgca
     1561 gtttgaaaaa ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca
     1621 gtttgagaaa atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga
     1681 agtcaagcct gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt
     1741 caagatcaaa aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg
     1801 taaggaaatg gactccttaa gattcttgta cgacggtatt agaatccaag ctgatcagac
     1861 ccctgaagat ttggacatgg aggataacga tattattgag gctcacagag aacagattgg
     1921 tggatccatg aaagtgacca aggtcgacgg catcagccac aagaagtaca tcgaagaggg
     1981 caagctcgtg aagtccacca gcgaggaaaa ccggaccagc gagagactga gcgagctgct
     2041 gagcatccgg ctggacatct acatcaagaa ccccgacaac gcctccgagg aagagaaccg
     2101 gatcagaaga gagaacctga agaagttctt tagcaacaag gtgctgcacc tgaaggacag
     2161 cgtgctgtat ctgaagaacc ggaaagaaaa gaacgccgtg caggacaaga actatagcga
     2221 agaggacatc agcgagtacg acctgaaaaa caagaacagc ttctccgtgc tgaagaagat
     2281 cctgctgaac gaggacgtga actctgagga actggaaatc tttcggaagg acgtggaagc
     2341 caagctgaac aagatcaaca gcctgaagta cagcttcgaa gagaacaagg ccaactacca
     2401 gaagatcaac gagaacaacg tggaaaaagt gggcggcaag agcaagcgga acatcatcta
     2461 cgactactac agagagagcg ccaagcgcaa cgactacatc aacaacgtgc aggaagcctt
     2521 cgacaagctg tataagaaag aggatatcga gaaactgttt ttcctgatcg agaacagcaa
     2581 gaagcacgag aagtacaaga tccgcgagta ctatcacaag atcatcggcc ggaagaacga
     2641 caaagagaac ttcgccaaga ttatctacga agagatccag aacgtgaaca acatcaaaga
     2701 gctgattgag aagatccccg acatgtctga gctgaagaaa agccaggtgt tctacaagta
     2761 ctacctggac aaagaggaac tgaacgacaa gaatattaag tacgccttct gccacttcgt
     2821 ggaaatcgag atgtcccagc tgctgaaaaa ctacgtgtac aagcggctga gcaacatcag
     2881 caacgataag atcaagcgga tcttcgagta ccagaatctg aaaaagctga tcgaaaacaa
     2941 actgctgaac aagctggaca cctacgtgcg gaactgcggc aagtacaact actatctgca
     3001 agtgggcgag atcgccacct ccgactttat cgcccggaac cggcagaacg aggccttcct
     3061 gagaaacatc atcggcgtgt ccagcgtggc ctacttcagc ctgaggaaca tcctggaaac
     3121 cgagaacgag aacgatatca ccggccggat gcggggcaag accgtgaaga acgccagcaa
     3181 cgttaccaac aagacagatc ctcgctccat gaactcccgt gtattcattg ggaatctcaa
     3241 cactcttgtg gtcaagaaat ctgatgtgga ggcaatcttt tcgaagtatg gcaaaattgt
     3301 gggctgctct gttcataagg gctttgcctt cgttcagtat gttaatgaga gaaatgcccg
     3361 ggctgctgta gcaggagagg atggcagaat gattgctggc caggttttag atattaacct
     3421 ggctgcagag ccaaaagtga accgaggaaa agcaggtgtg aaacgatctg cagcggagat
     3481 gtacgggaac aagggcgaag agaaatacgt gtccggcgag gtggacaaga tctacaatga
     3541 gaacaagcag aacgaagtga aagaaaatct gaagatgttc tacagctacg acttcaacat
     3601 ggacaacaag aacgagatcg aggacttctt cgccaacatc gacgaggcca tcagcagcat
     3661 cagacacggc atcgtgcact tcaacctgga actggaaggc aaggacatct tcgccttcaa
     3721 gaatatcgcc cccagcgaga tctccaagaa gatgtttcag aacgaaatca acgaaaagaa
     3781 gctgaagctg aaaatcttca agcagctgaa cagcgccaac gtgttcaact actacgagaa
     3841 ggatgtgatc atcaagtacc tgaagaatac caagttcaac ttcgtgaaca aaaacatccc
     3901 cttcgtgccc agcttcacca agctgtacaa caagattgag gacctgcgga ataccctgaa
     3961 gtttttttgg agcgtgccca aggacaaaga agagaaggac gcccagatct acctgctgaa
     4021 gaatatctac tacggcgagt tcctgaacaa gttcgtgaaa aactccaagg tgttctttaa
     4081 gatcaccaat gaagtgatca agattaacaa gcagcggaac cagaaaaccg gccactacaa
     4141 gtatcagaag ttcgagaaca tcgagaaaac cgtgcccgtg gaatacctgg ccatcatcca
     4201 gagcagagag atgatcaaca accaggacaa agaggaaaag aatacctaca tcgactttat
     4261 tcagcagatt ttcctgaagg gcttcatcga ctacctgaac aagaacaatc tgaagtatat
     4321 cgagagcaac aacaacaatg acaacaacga catcttctcc aagatcaaga tcaaaaagga
     4381 taacaaagag aagtacgaca agatcctgaa gaactatgag aagcacaatc ggaacaaaga
     4441 aatccctcac gagatcaatg agttcgtgcg cgagatcaag ctggggaaga ttctgaagta
     4501 caccgagaat ctgaacatgt tttacctgat cctgaagctg ctgaaccaca aagagctgac
     4561 caacctgaag ggcagcctgg aaaagtacca gtccgccaac aaagaagaaa ccttcagcga
     4621 cgagctggaa ctgatcaacc tgctgaacct ggacaacaac agagtgaccg aggacttcga
     4681 gctggaagcc aacgagatcg gcaagttcct ggacttcaac gaaaacaaaa tcaaggaccg
     4741 gaaagagctg aaaaagttcg acaccaacaa gatctatttc gacggcgaga acatcatcaa
     4801 gcaccgggcc ttctacaata tcaagaaata cggcatgctg aatctgctgg aaaagatcgc
     4861 cgataaggcc aagtataaga tcagcctgaa agaactgaaa gagtacagca acaagaagaa
     4921 tgagattgaa aagaactaca ccatgcagca gaacctgcac cggaagtacg ccagacccaa
     4981 gaaggacgaa aagttcaacg acgaggacta caaagagtat gagaaggcca tcggcaacat
     5041 ccagaagtac acccacctga agaacaaggt ggaattcaat gagctgaacc tgctgcaggg
     5101 cctgctgctg aagatcctgc accggctcgt gggctacacc agcatctggg agcgggacct
     5161 gagattccgg ctgaagggcg agtttcccga gaaccactac atcgaggaaa ttttcaattt
     5221 cgacaactcc aagaatgtga agtacaaaag cggccagatc gtggaaaagt atatcaactt
     5281 ctacaaagaa ctgtacaagg acaatgtgga aaagcggagc atctactccg acaagaaagt
     5341 gaagaaactg aagcaggaaa aaaaggacct gtacatccgg aactacattg cccacttcaa
     5401 ctacatcccc cacgccgaga ttagcctgct ggaagtgctg gaaaacctgc ggaagctgct
     5461 gtcctacgac cggaagctga agaacgccat catgaagtcc atcgtggaca ttctgaaaga
     5521 atacggcttc gtggccacct tcaagatcgg cgctgacaag aagatcgaaa tccagaccct
     5581 ggaatcagag aagatcgtgc acctgaagaa tctgaagaaa aagaaactga tgaccgaccg
     5641 gaacagcgag gaactgtgcg aactcgtgaa agtcatgttc gagtacaagg ccctggaata
     5701 agcggccgca ctcgaggccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     5761 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     5821 aactatatcc ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
     5881 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
     5941 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
     6001 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     6061 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6121 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6181 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6241 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6301 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6361 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6421 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     6481 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6541 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6601 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6661 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6721 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     6781 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6841 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6901 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggatctc gcggtatcat
     6961 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7021 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7081 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7141 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7201 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7261 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7321 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7381 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7441 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7501 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7561 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7621 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7681 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7741 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7801 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7861 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7921 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7981 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     8041 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
     8101 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     8161 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     8221 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     8281 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     8341 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     8401 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     8461 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     8521 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     8581 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     8641 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     8701 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     8761 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     8821 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     8881 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     8941 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     9001 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     9061 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     9121 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     9181 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     9241 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     9301 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     9361 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     9421 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     9481 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     9541 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     9601 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     9661 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     9721 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     9781 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9841 cgcggggaga ggcggtt
//
