LOCUS       sequence-380262-        9237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of YAP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX304 YAP1
FEATURES             Location/Qualifiers
     source          1..9237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1944..2399)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2081..2102)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2293..2312
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2526..2548
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2540..2557
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2541..2557
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2567..2586
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2567..2585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2606..2741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2781..2902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2792..2811)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2846..2865
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2980..3213
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3285..3873
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3391..3402
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3800..3820
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3915..3956)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3958..3982
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5498..5522)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(5545..5748)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5574..5594)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5749..6052
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6169..6286
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6346..6741)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6753..7253)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6816..6834)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7428..7472)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7657..7890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8383..8508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8555..8735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8736..8962)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8990..9010)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8990..9008)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9029..9045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9029..9045)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9042..9064)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9053..9069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9077..9083,9084..9101,9102..9107))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9122..9143
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     1981 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2041 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2101 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2161 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2221 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2281 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2341 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     2401 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2461 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2521 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     2581 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
     2641 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     2701 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     2761 atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2821 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2881 tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata
     2941 aatactgaat aataagatga catgaactac tatacgtact gctagagatt ttccacactg
     3001 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     3061 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     3121 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat
     3181 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     3241 taagatctac agctgccttg taagtcattg gtcttaaagt cgacgcgggg aggcggccca
     3301 aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg
     3361 cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga
     3421 cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt
     3481 ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
     3541 aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga
     3601 aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt
     3661 gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca
     3721 ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg
     3781 cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa
     3841 tctttcacaa attttgtaat ccagaggttg attgtcgact taacgcgcca ccggttagcg
     3901 ctagctcatt actacgtaga atcgagaccg aggagagggt tagggatagg cttaccaacc
     3961 actttgtaca agaaagttgg gggtggtaac catgtaagaa agctttcttt atctagcttg
     4021 gtggcagcca aaacagactc catgtcatta aggatgtcag aactcaaagc ttcctgcaga
     4081 cttggcatca gctcctctcc ttctatgttc attccatctc cttccagtgt tccaaggtcc
     4141 acatttgtcc caggaatggc ttcaaggtag tctgggaaac ggttctgctg tgagggcagg
     4201 gtgctttggt tgatagtatc acctgtatcc atctcatcca cactgttcag gaagtcatct
     4261 ggggttcgag ggacactgta gctgctcatg cttagtccac tgtctgtact ctcatctcga
     4321 gagtgatagg tgccactgtt aaggaaagga tctgagctat tggtcgtcat tgttctcaat
     4381 tcctgagaca tcccgggaga agacactgga ttttgagtcc caccatcctg ctccagtgtt
     4441 ggtaactggc tacgcagggc taactcctga cattttggag aatttgctgt gctgggattg
     4501 atattccgca ttgcctgccg aagcagttct tgctgtttca gccgcagcct ctccttctcc
     4561 atctgcagtt gctgcagtcg catctgttgc tgctggttgg agttgctgcc acccatgacg
     4621 cctccctgtg ggctctgggg agccaggggt ggtggctgtt tcactggagc actctgactg
     4681 attctctggt tcatggcaaa acgagggtca agccttgggt ctagccaaga ggtggtcttg
     4741 ttcttatggt ttatatagta aatttctcca tcctgagtca tggcttgttc ccatccatca
     4801 ggaagaggac ctgaagccga gttcatcata ttctgctgca ctggtggact ggtgggggct
     4861 gtgacgttca tctgggacag catggccttc ctggggtcct gccatgttgt tgtctgatcg
     4921 atgtgattta agaagtatct ctgaccagaa gatgtctttg ccatctccca acctgctggc
     4981 agaggtacat catcaggtat ctcaaaagaa gactgtcgaa gatgctgagc tgtgggtgta
     5041 gctgctgggc cagagactac tccagtgggg gtcagtgtcc caggagaaac agctcccaac
     5101 tgcagagaag ctggagagga atgagctcga acatgctgtg gagtcagggc tcctgcagtg
     5161 cctgcatcag tactggcctg tcgggagtgg gatttgggct ccggcggctt gaagaaggag
     5221 tcgggcagct tccggagcct catgggcacg gtctggggca cgttggccgt cttggggttc
     5281 atgacggcgt tgaagagcgc ctccaggtcg gtctccgagt ccccgcggac gtgcacgatc
     5341 tgatgcccgg cggggggtgc ctgcggcgcc gcctgggtcg ccgcgggtgc cggttgcccg
     5401 ggtccggacg gcgggccctg cccctgcggg ggctgcgaag gcggctgccc ttggccctgg
     5461 ggggccggtt gaggcggcgg ctgctgcccg ggatccatgc caactttttt gtacaaactt
     5521 gttgatcccg acagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
     5581 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     5641 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     5701 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     5761 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     5821 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     5881 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     5941 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     6001 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     6061 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac cccgggcccc
     6121 ctcgagccgg cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     6181 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     6241 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     6301 tgccatttgt ctcaagatct agaattctta ctactgcagc tgcaggccct cccacacata
     6361 accagagggc agcaattcac gaatcccaac tgccgtcggc tgtccatcac tgtccttcac
     6421 tatggctttg atcccaggat gcagatcgag aagcacctgt cggcaccgtc cgcaggggct
     6481 caagatgccc ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc
     6541 agcagcagca gtgcccagca ccacgagttc tgcacaaggt cccccagtaa aatgatatac
     6601 attgacacca gtgaagatgc ggccgtcgct agagagagct gcgctggcga cgctgtagtc
     6661 ttcagagatg gggatgctgt tgattgtagc cgttgctctt tcaatgaggg tggattcttc
     6721 ttgagacaaa ggcttggcca tggtggtacc ccctggggag agaggtcggt gattcggtca
     6781 acgagggagc cgactgccga cgtgcgctcc ggaggcttgc agaatgcgga acaccgcgcg
     6841 ggcaggaaca gggcccacac taccgcccca caccccgcct cccgcaccgc cccttcccgg
     6901 ccgctgctct cggcgcgccc tgctgagcag ccgctattgg ccacagccca tcgcggtcgg
     6961 cgcgctgcca ttgctccctg gcgctgtccg tctgcgaggg tactagtgag acgtgcggct
     7021 tccgtttgtc acgtccggca cgccgcgaac cgcaaggaac cttcccgact taggggcgga
     7081 gcaggaagcg tcgccggggg gcccacaagg gtagcggcga agatccgggt gacgctgcga
     7141 acggacgtga agaatgtgcg agacccaggg tcggcgccgc tgcgtttccc ggaaccacgc
     7201 ccagagcagc cgcgtccctg cgcaaaccca gggctgcctt ggaaaaggcg caagggtccc
     7261 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
     7321 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
     7381 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
     7441 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     7501 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     7561 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     7621 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     7681 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     7741 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     7801 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     7861 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     7921 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     7981 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     8041 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     8101 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     8161 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     8221 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     8281 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     8341 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     8401 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     8461 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     8521 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     8581 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     8641 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     8701 tcccaggctc agatctggtc taaccagaga gacccgttta tgtatcgagc taggcactta
     8761 aatacaatat ctctgcaatg cggcaattca gtggttcgtc caatccatgt cagacccgtc
     8821 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
     8881 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
     8941 aagagtattg cataagacta cattaagctt gcagctccag cttttgttcc ctttagtgag
     9001 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9061 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9121 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9181 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgc
//
