LOCUS       sequence-380263-        9435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of PRDM14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX304 PRDM14
FEATURES             Location/Qualifiers
     source          1..9435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        103..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    633..657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2371..2395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2397..2438
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2480..3068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2533..2553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2951..2962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3140..3373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3451..3572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3488..3507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3542..3561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3612..3747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3767..3786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3768..3786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3796..3813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3796..3812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3805..3827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3954..4409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4041..4060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4251..4272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4435..4539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4540..4608,4609..5400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4758..4777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5571..6159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6060..6079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6313..6330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6447..6468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6483..6488,6489..6506,6507..6513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6521..6537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6526..6548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6545..6561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6545..6561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6580..6600
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6582..6600
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6628..6854
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6855..7035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7082..7207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7700..7933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8118..8162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8337..8837
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8756..8774
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8849..9244
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9304..9421
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacagcaga gatccacttt ggcgccggct cgagggggcc cggggttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctgtcgggat caacaagttt gtacaaaaaa gttggcatgg
      661 ctctaccccg gccaagtgag gccgtgcctc aggacaaggt gtgctacccg ccggagagca
      721 gcccgcagaa cctggccgcg tactacacgc ctttcccgtc ctatggacac tacagaaaca
      781 gcctggccac cgtggaggaa gacttccaac ctttccggca gctggaggcc gcagcgtctg
      841 ctgcccccgc catgcccccc ttccccttcc ggatggcgcc tcccttgctg agcccgggtc
      901 tgggcctaca gagggagcct ctctacgatc tgccctggta cagcaagctg ccaccgtggt
      961 acccaattcc ccacgtcccc agggaagtgc cgcccttcct gagcagcagc cacgagtacg
     1021 cgggtgccag cagtgaagat ctgggccacc aaatcattgg tggcgacaac gagagtggcc
     1081 cgtgttgtgg acctgacact ttaattccac cgccccctgc ggatgcttct ctgttacctg
     1141 aggggctgag gacctcccag ttattacctt gctcacccag caagcagtca gaggatggtc
     1201 ccaaaccctc caaccaagaa gggaagtccc ctgctcggtt ccagttcacg gaggaggacc
     1261 tgcacttcgt tctgtacggg gtcactccca gcctggagca cccagccagc ctgcaccatg
     1321 cgatttcagg cctcctggtc cccccagaca gctctggatc tgattctctt cctcaaactc
     1381 tggatgaaga ctcccttcaa cttccagaag gtctatgcct catgcagacg gtgtttggtg
     1441 aagtcccaca ttttggtgtg ttctgcagta gttttatcgc caaaggagtc aggtttgggc
     1501 cctttcaagg taaagtggtc aatgccagtg aagtgaagac ctacggagac aattctgtga
     1561 tgtgggagat ctttgaagat ggtcatttga gccactttat agatggaaaa ggaggtacgg
     1621 ggaactggat gtcctatgtc aactgtgccc gcttccccaa ggagcagaac ctagttgctg
     1681 tgcagtgtca agggcatata ttttatgaga gctgcaaaga gattcatcag aaccaagagc
     1741 tccttgtgtg gtatggagac tgctatgaga aatttctgga tattcctgtg agccttcagg
     1801 tcacagagcc ggggaagcag ccatctgggc cctctgaaga gtctgcagaa ggctacagat
     1861 gtgaaagatg tgggaaggta tttacctaca aatattacag agataagcac ctcaagtaca
     1921 ccccctgtgt ggacaagggc gataggaaat ttccctgttc tctctgcaaa cgatcctttg
     1981 agaagcggga ccggcttcgg atccacattc ttcatgttca tgagaagcac cggcctcaca
     2041 agtgttctac atgtgggaaa tgtttctctc agtcttccag cctaaacaaa cacatgcgag
     2101 tccactctgg agacagacca taccagtgtg tgtattgtac taagaggttc acagcctcca
     2161 gcatactccg cacacacatc aggcagcact ccggggagaa gcccttcaaa tgcaagtact
     2221 gtggtaaatc ttttgcatcc catgctgccc atgacagcca tgtccggcgt tcacacaagg
     2281 aggacgatgg ctgctcatgc agcatctgtg ggaaaatctt ctcagatcaa gaaacattct
     2341 actcccacat gaagtttcat gaagactact acccaacttt cttgtacaaa gtggttggta
     2401 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
     2461 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2521 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2581 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2641 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2701 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2761 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2821 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2881 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2941 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3001 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3061 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3121 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3181 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3361 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3421 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3481 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3541 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3601 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3661 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3721 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3781 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3961 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4021 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4081 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4141 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4201 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4261 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4321 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4381 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4501 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4561 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4621 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4681 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4741 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4801 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4861 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4921 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4981 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5041 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5101 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5161 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5221 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5281 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5341 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5401 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5461 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6181 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6241 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6301 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6361 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6421 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6481 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6541 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6601 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6661 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6721 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6781 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6841 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7021 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7081 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7141 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7201 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7261 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7321 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7381 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7441 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7501 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     7561 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7621 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7681 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7741 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7801 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7861 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7921 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7981 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8041 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8101 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8161 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8221 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8281 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     8341 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     8401 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     8461 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     8521 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     8581 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     8641 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     8701 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     8761 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     8821 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     8881 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     8941 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     9001 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     9061 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     9121 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     9181 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     9241 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
     9301 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     9361 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     9421 tcgggtttat tacag
//
