LOCUS       sequence-380264-        8478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX304 GFP
FEATURES             Location/Qualifiers
     source          1..8478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        413..616
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     567..587
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    639..663
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(684..686,687..689,690..1400)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(729..750)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(990..1009)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1337..1358
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1420..1444)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1446..1487
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1529..2117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1582..1602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2000..2011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2189..2422
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2500..2621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2537..2556)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2591..2610
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2661..2796
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2816..2835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2817..2835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2845..2862)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2845..2861)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2854..2876)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3003..3458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3090..3109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3300..3321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3484..3588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3589..3657,3658..4449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3807..3826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4620..5208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5109..5128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5362..5379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5496..5517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5532..5537,5538..5555,5556..5562)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5570..5586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5575..5597
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5594..5610
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5594..5610
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5629..5649
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5631..5649
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5677..5903
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5904..6084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6131..6256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6749..6982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7167..7211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7386..7886
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7805..7823
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7898..8293
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8353..8470
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg gttattaata
       61 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      421 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      481 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      541 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      601 ctatataagc agagctctct ggctaactgt cgggatcaac aagtttgtac aaaaaagcag
      661 gctccgcggc cgcccccttc accatggtga gcaagggcga ggagctgttc accggggtgg
      721 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtctggcg
      781 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
      841 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
      901 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
      961 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     1021 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     1081 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     1141 tcatggccga caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg
     1201 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     1261 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     1321 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     1381 gcatggacga gctgtacaag tccaagggtg ggcgcgccga cccagctttc ttgtacaaag
     1441 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     1501 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     1561 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1621 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1681 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     1741 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     1801 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     1861 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     1921 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     1981 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2041 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2101 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2161 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2221 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2281 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2341 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2401 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     2461 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     2521 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2581 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     2641 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2701 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2761 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     2821 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2881 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2941 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3001 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3061 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3361 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3421 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     3781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     3841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     3901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     3961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4741 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4981 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5401 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5461 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5521 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5581 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     5641 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     5701 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     5761 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     5821 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     5881 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     5941 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6001 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6061 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6121 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6181 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6241 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6301 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6361 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6421 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6481 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     6541 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     6601 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6661 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6721 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     6781 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     6841 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     6901 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     6961 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7021 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7081 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7141 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7201 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7261 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7321 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7381 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     7441 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     7501 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     7561 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     7621 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     7681 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     7741 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     7801 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     7861 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     7921 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     7981 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     8041 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     8101 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     8161 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     8221 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     8281 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     8341 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8401 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8461 tcaaaatttt cgggttta
//
