LOCUS       sequence-380265-        2550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPRAduo backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Mouri K, Dewey HB, Castro R, Berenzy D, Kales S, Tewhey R
  TITLE     Whole-genome functional characterization of RE1 silencers using a 
            modified massively parallel reporter assay.
  JOURNAL   Cell Genom. 2022 Dec 16;3(1):100234. doi: 
            10.1016/j.xgen.2022.100234. eCollection 2023 Jan 11.
  PUBMED    36777181
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-16"; volume: "3(1):100234. doi"; pages: " 
            10.1016/j.xgen.2022.100234. eCollection 2023 Jan 11"
COMMENT     Sequence Label: pMPRAduo:∆orf
FEATURES             Location/Qualifiers
     source          1..2550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(5..796,797..865))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    970..1018
                     /note="synthetic polyadenylation signal"
     misc_feature    1032..1123
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1072..1091
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    1227..1348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1238..1257)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1292..1311
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1596..1613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1767..2355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1847..1866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
       61 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
      121 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
      181 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
      241 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
      301 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
      361 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
      421 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
      481 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
      541 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
      601 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
      661 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
      721 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
      781 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
      841 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
      901 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
      961 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     1021 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     1081 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggccggta
     1141 cctgagctcg ctagcctcga ggatatcaag atctggccgc cgaggcctag tgagtaccgg
     1201 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1261 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1321 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1381 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     1441 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     1501 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     1561 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2521 gacagcggcc gcaaatgcta aaccactgca
//
