LOCUS       sequence-380266-        7908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Liu B, Stone OJ, Pablo M, Herron JC, Nogueira AT, Dagliyan O, Grimm 
            JB, Lavis LD, Elston TC, Hahn KM
  TITLE     Biosensors based on peptide exposure show single molecule 
            conformations in live cells.
  JOURNAL   Cell. 2021 Oct 28;184(22):5670-5685.e23. doi: 
            10.1016/j.cell.2021.09.026. Epub 2021 Oct 11.
  PUBMED    34637702
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09.026"; journalName: "Cell"; date: 
            "2021-10-28- 28"; volume: "184"; issue: "22"; pages: "5670-5685.e23"
COMMENT     Sequence Label: binder/tag-WT-FAK-insertionB
FEATURES             Location/Qualifiers
     source          1..7908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             657..1364
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(802..820)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1070..1089
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4554..4580
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4584..4610
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4740..4861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4777..4796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4831..4850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4868..5323)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5005..5026)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5217..5236
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5350..5454
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5456..5813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5456..5647
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5479..5499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5664..5799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5848..6642
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5848..6642
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5902..5921)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6512..6531
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6830..6849)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6874..6921
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7250..7838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7739..7758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg
      661 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      721 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      781 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      841 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      901 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      961 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     1021 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1081 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1141 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1201 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     1261 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1321 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccggc atggcagctg
     1381 cttatcttga cccaaacttg aatcacacac caagttcgag tactaagact cacctgggta
     1441 ctggcatgga acggtcccct ggtgcaatgg aacgagtatt aaaggtcttt catcattttg
     1501 aaagcagtag tgagccaacc acctgggcca gtattatcag gcatggagat gctactgatg
     1561 tcaggggcat cattcagaag atagtggaca gtcacaaagt aaagcacgtg gcctgctatg
     1621 gatttcgcct cagtcacctg cgatcggagg aggtgcactg gctgcacgtg gacatgggtg
     1681 tctccagtgt gagggagaaa tatgaactcg cccacccacc agaggagtgg aaatatgagt
     1741 tgagaattcg ttacttgcca aaaggatttc taaaccagtt tactgaagat aagccaacat
     1801 tgaatttctt ctatcaacag gtgaagagtg actacatgca agaaatagct gatcaagtag
     1861 accaagaaat agctttgaag ttgggttgtt tggaaattag gcgatcctat tgggagatga
     1921 ggggtaatgc cctagagaag aagtccaact atgaagtatt agaaaaagat gttggtttaa
     1981 agcgattttt tcctaagagt ttactggatt ctgtcaaggc caaaacacta agaaagctga
     2041 tccagcaaac attcaggcag tttgccaacc ttaatagaga agaaagcatc ctgaaattct
     2101 ttgagattct ttctcctgtg tacagatttg acaaagagtg cttcaagtgt gcccttgggt
     2161 caagttggat catttctgtg gaattggcaa tcggcccaga agaagggatc agttacctga
     2221 cagacaaagg ctgcaatccc acacacctag cagactttaa ccaagtgcag accatccagt
     2281 actcaaacag tgaagacaaa gacaggaaag gaatgctaca actcaaaatc gctggtgctc
     2341 ctgagcctct gaccgtgacg gcaccatccc taaccattgc cgagaacatg gccgacctga
     2401 ttgatggata ctgccggctg gtgaatggag ccacacaatc tttcatcatc agaccccaga
     2461 aagaaggtga acgggcgttg ccatcaatac caaagttggc caacagtgaa aagcaaggca
     2521 tgcggacaca tgcagtctct gtgtcagaga cagatgacta tgcagagatc atcgatgagg
     2581 aagacgctgc aaatgatgaa aactacacat acaccatgcc ctcgaccagg gattatgaga
     2641 ttcagagaga aagaatagaa cttggacgct gtattggaga aggtcagttt ggagatgtac
     2701 atcaaggcgt gtacctgagc ccagagaatc cagctttggc tgttgcaatc aaaacatgta
     2761 aaaactgtac ttcggacagc gtgagagaga agttccttca agaagcctta acaatgcgtc
     2821 agtttgacca tcctcacatc gtgaagctga ttggagtcat tacagagaac cctgtctgga
     2881 taatcatgga gttgtgcaca cttggagagc tgaggtcatt tttgcaagta agaaaataca
     2941 gcttggacct ggcatctttg atattatatg cctatcagct tagtacagca ctcgcgtatc
     3001 tggagagcaa aagatttgtt cacagggaca ttgctgctcg gaatgttctg gtgtcttcaa
     3061 atgattgtgt aaaattggga gactttggat tatctcgata tatggaagac agtacttact
     3121 ataaagcttc caaaggaaaa ttacctatta aatggatggc tccagagtca atcaattttc
     3181 gacgttttac ctcagctagt gacgtgtgga tgtttggtgt gtgtatgtgg gagatactga
     3241 tgcatggcgt gaagcctttt caaggagtga agaacaatga tgtgatcggt cgaattgaaa
     3301 atggggaaag attaccaatg cctccaaatt gtcctcccac cctctacagc cttatgacga
     3361 aatgttgggc ctatgacccc agcaggcggc ccaggtttac tgaactaaaa gctcagctca
     3421 gcacaatcct ggaggaggag aaggtgcagc aagaagaacg gatgaggatg gaatccagaa
     3481 gacaggctac cgtgtcctgg gactctggag ggtctgatga agcaccaccc aagcccagca
     3541 gacctggtta tcctagcccg agatccagtg aaggatttta tcccagccca cagcacatgg
     3601 tccagaccaa tcactaccag gtctctggct acccgggttc ccatggaatc ccagccatgg
     3661 ctggcagcat ttaccaaggt caggcatctc ttctggacca gacagaattg tggaatcaca
     3721 gacctcagga gatgtccatg tggcagccca gtgtggagga ttcggcagct ttggaccttc
     3781 gagggatggg acaggtgctt ccccctcacc tgatggaaga gcggctaatc cgacagcagc
     3841 aggaaatgga agaagaccag cgctggctgg agaaggaaga gcgattcctg aaacctgatg
     3901 tgaggctctc tcgaggcagc atcgacaggg aagacgggag ttttcagggt ccgactggaa
     3961 accaacacat ctatcagcct gtggggaagc cagatcctgc agctccacca aagaaacctc
     4021 ctcgccctgg agcacctggc cacctaagca acctgtccag catcagcagc cctgcagaca
     4081 gctacaatga gggtgtcaag cttcagcccc aggaaatcag cccccctccc actgccaacc
     4141 ttgaccggtc caatgacaag gtatatgaaa atgtgacagg cctagtgaag gctgtcatcg
     4201 agatgtccag caaaatccag ccagctcctc cagaagagta cgtccctatg gtgaaggaag
     4261 ttggcctggc cctgcggacc ttactagcca cggtggatga gaccattcct gctcttccag
     4321 ccagcactca tcgagagatc gagatggcac agaagctgct gaactccgac ttaggcgagc
     4381 tcatcagcaa gatgaagctg gcgcagcagt acgtcatgac cagcctgcag caggagtata
     4441 agaagcagat gctgacagct gctcacgccc tggctgtgga tgccaagaac ctacttgatg
     4501 ttattgatca agcaagactg aaaatgctag ggcagacacg cggccgcatc ttttacccat
     4561 acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca ggatccaccg
     4621 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4681 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4861 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4921 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4981 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     5041 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     5101 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     5161 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     5221 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     5281 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     5461 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5581 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5641 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5701 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5761 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     5821 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5881 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5941 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     6001 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     6061 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     6121 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     6181 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     6241 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     6301 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     6361 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6421 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6481 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6541 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6601 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6661 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6721 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6781 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     6841 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     6901 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     6961 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     7021 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     7081 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7381 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7441 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7501 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7561 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7621 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7681 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7741 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7801 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7861 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctg
//
