LOCUS       sequence-38027-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3545 (=HL 770 + pHL 1213)
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(220..306)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    671..687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(739..826)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacgc cctaggtcta gggcggcgga tttgtcctac tcaggagagc
      241 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      301 tatttgatgc ctctagcacg cgtaccatgg gcccttattc ggtttcttcg ctgtcctgtt
      361 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
      421 cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt
      481 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
      541 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
      601 gtgcgttcag gaacggatct tgtaaagatt gccccttagc catttttttt cctccttacc
      661 cgggagtatc ttgttatccg ctcacaatgt caatgttatc cgctcacatt tatggatccc
      721 ctaggtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      781 aaaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc
      841 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
      901 ttcggcatgg ggtc
//
