LOCUS       sequence-380282-        9811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of TEAD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX317 TEAD2
FEATURES             Location/Qualifiers
     source          1..9811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        129..1307
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          360..1298
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1255..1275
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1327..1351
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2693..2717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2725..2766
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2877..3465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2930..2950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3348..3359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3537..3770
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3848..3969
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3885..3904)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3939..3958
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4009..4144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4164..4183)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4165..4183)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4193..4210)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4193..4209)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4202..4224)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4351..4806
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4438..4457)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4648..4669
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4832..4936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4937..5005,5006..5797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5155..5174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5968..6556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6457..6476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6710..6727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6844..6865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6880..6885,6886..6903,6904..6910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6918..6934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6923..6945
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6942..6958
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6942..6958
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6977..6997
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6979..6997
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7025..7251
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7252..7432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7479..7604
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8097..8330
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8515..8559
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8790..9000
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8812..9008
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8859..8994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9040..9642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9040..9059)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9536..9556
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9685..9802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacagggaca gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga
       61 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      121 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      181 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
      241 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      301 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      361 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      421 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
      481 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
      541 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
      601 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
      661 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
      721 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
      781 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
      841 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
      901 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
      961 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     1021 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     1081 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     1141 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     1201 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     1261 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaggc tagcatcgat
     1321 tgatcaacaa gtttgtacaa aaaagttggc atgggggaac cccgggctgg ggccgccctg
     1381 gacgatggca gcggctggac gggcagtgag gaaggcagtg aggagggtac cggcggcagt
     1441 gagggggctg ggggtgacgg gggcccggat gcagaggggg tgtggagccc agacattgag
     1501 cagagcttcc aggaggccct ggccatctat ccaccctgcg gccgccggaa aataattttg
     1561 tctgatgaag gcaagatgta tggtcggaat gaactgatcg cccgctacat caagctgaga
     1621 acggggaaga cccgaactcg aaaacaggtt tctagtcaca tccaggtttt ggcccgaagg
     1681 aaatcaaggg aaatccagtc caagttgaag gaccaggttt ccaaggacaa ggctttccag
     1741 acaatggcaa ccatgtcctc tgcccagctc atctccgcgc cttctctgca ggccaaactg
     1801 ggtcccactg gtcctcaggc ctctgagctt ttccagtttt ggtctggagg atctgggccc
     1861 ccctggaatg ttccagatgt gaagccattc tcacagacac cgttcacctt gtcactgact
     1921 cccccatcta ctgacctccc agggtacgag cccccccaag ccctctcacc cctgccccca
     1981 cctaccccat cgcccccagc ctggcaggct cggggcctgg gcaccgcccg gttgcagctg
     2041 gtagagttct cagccttcgt ggaaccgcca gatgcagttg attcttacca gaggcacctg
     2101 ttcgtgcaca tcagccagca ctgccccagc cccggagcgc cgccgctcga gagtgtggac
     2161 gtccggcaga tctacgacaa attccctgag aaaaagggtg gcctccgaga gctatatgat
     2221 cgtggccccc cccatgcctt cttcctggtc aagttctggg cggacctgaa ctggggccca
     2281 agtggtgagg aggcaggggc cggtggcagc atcagcagtg gtggcttcta cggagtgagc
     2341 agccagtatg agagcctgga acacatgacc ctcacctgtt cctccaaggt ctgctctttt
     2401 ggcaagcagg tggtggagaa ggtggagacg gaacgggccc agctggagga cggcagattt
     2461 gtgtaccgcc tgctgcgctc gcccatgtgc gagtacctgg tgaatttctt gcacaagttg
     2521 cggcagctgc ctgagcgata catgatgaac agcgtcctgg aaaacttcac catcctccag
     2581 gtggtgacaa acagagacac ccaggaactg ctgctctgca ccgcctatgt cttcgaggtc
     2641 tccaccagcg agcgtggggc ccagcatcac atttaccgcc tggtcaggga ctgcccaact
     2701 ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct cggtctcgat
     2761 tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2821 tcttgtggaa aggacgagga cgtccgaagg agaccatatt cacgcgttaa gtcgacaatc
     2881 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2941 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3001 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3061 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3121 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3181 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3241 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3301 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3361 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3421 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     3481 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3541 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3601 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3661 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3721 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     3781 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     3841 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     3901 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     3961 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     4021 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4081 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4141 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     4201 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4261 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4321 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4381 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4441 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4501 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4561 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4621 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4681 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4741 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4801 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5161 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6721 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     6961 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     7021 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     7081 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     7141 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     7201 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7441 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7501 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7561 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7621 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7681 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7741 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7801 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7861 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7921 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     7981 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8041 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8101 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8161 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8221 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8281 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8341 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8401 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8461 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8521 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8581 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8641 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8701 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     8761 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     8821 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8941 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9001 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     9061 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     9121 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     9181 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     9241 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     9301 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     9361 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     9421 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     9481 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     9541 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     9601 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     9661 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9721 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9781 aattacaaaa attcaaaatt ttcgggttta t
//
