LOCUS       sequence-380283-        9949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of SLC2A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX317 SLC2A1
FEATURES             Location/Qualifiers
     source          1..9949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..1320
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          373..1311
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1268..1288
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1340..1364
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2844..2868)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2876..2917
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3028..3616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3081..3101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3499..3510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3688..3921
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3999..4120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4036..4055)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4090..4109
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4160..4295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4315..4334)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4316..4334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4344..4361)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4344..4360)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4353..4375)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4502..4957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4589..4608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4799..4820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4983..5087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5088..5156,5157..5948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5306..5325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6119..6707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6608..6627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6861..6878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6995..7016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7031..7036,7037..7054,7055..7061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7069..7085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7074..7096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7093..7109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7093..7109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7128..7148
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7130..7148
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7176..7402
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7403..7583
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7630..7755
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8248..8481
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8666..8710
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8941..9151
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8963..9159
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9010..9145
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9191..9793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9191..9210)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9687..9707
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(9836..9949,1..4)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg aggcctgcag
       61 gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
      121 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
      181 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
      241 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
      301 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
      361 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
      421 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
      481 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
      541 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
      601 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
      661 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
      721 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
      781 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
      841 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
      901 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
      961 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     1021 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     1081 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     1141 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     1201 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     1261 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     1321 ggctagcatc gattgatcaa caagtttgta caaaaaagtt ggcaccatgg agcccagcag
     1381 caagaagctg acgggtcgcc tcatgctggc cgtgggagga gcagtgcttg gctccctgca
     1441 gtttggctac aacactggag tcatcaatgc cccccagaag gtgatcgagg agttctacaa
     1501 ccagacatgg gtccaccgct atggggagag catcctgccc accacgctca ccacgctctg
     1561 gtccctctca gtggccatct tttctgttgg gggcatgatt ggctccttct ctgtgggcct
     1621 tttcgttaac cgctttggcc ggcggaattc aatgctgatg atgaacctgc tggccttcgt
     1681 gtccgccgtg ctcatgggct tctcgaaact gggcaagtcc tttgagatgc tgatcctggg
     1741 ccgcttcatc atcggtgtgt actgcggcct gaccacaggc ttcgtgccca tgtatgtggg
     1801 tgaagtgtca cccacagccc ttcgtggggc cctgggcacc ctgcaccagc tgggcatcgt
     1861 cgtcggcatc ctcatcgccc aggtgttcgg cctggactcc atcatgggca acaaggacct
     1921 gtggcccctg ctgctgagca tcatcttcat cccggccctg ctgcagtgca tcgtgctgcc
     1981 cttctgcccc gagagtcccc gcttcctgct catcaaccgc aacgaggaga accgggccaa
     2041 gagtgtgcta aagaagctgc gcgggacagc tgacgtgacc catgacctgc aggagatgaa
     2101 ggaagagagt cggcagatga tgcgggagaa gaaggtcacc atcctggagc tgttccgctc
     2161 ccccgcctac cgccagccca tcctcatcgc tgtggtgctg cagctgtccc agcagctgtc
     2221 tggcatcaac gctgtcttct attactccac gagcatcttc gagaaggcgg gggtgcagca
     2281 gcctgtgtat gccaccattg gctccggtat cgtcaacacg gccttcactg tcgtgtcgct
     2341 gtttgtggtg gagcgagcag gccggcggac cctgcacctc ataggcctcg ctggcatggc
     2401 gggttgtgcc atactcatga ccatcgcgct agcactgctg gagcagctac cctggatgtc
     2461 ctatctgagc atcgtggcca tctttggctt tgtggccttc tttgaagtgg gtcctggccc
     2521 catcccatgg ttcatcgtgg ctgaactctt cagccagggt ccacgtccag ctgccattgc
     2581 cgttgcaggc ttctccaact ggacctcaaa tttcattgtg ggcatgtgct tccagtatgt
     2641 ggagcaactg tgtggtccct acgtcttcat catcttcact gtgctcctgg ttctgttctt
     2701 catcttcacc tacttcaaag ttcctgagac taaaggccgg accttcgatg agatcgcttc
     2761 cggcttccgg caggggggag ccagccaaag tgacaagaca cccgaggagc tgttccatcc
     2821 cctgggggct gattcccaag tgttgccaac tttcttgtac aaagtggttg atatcggtaa
     2881 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact
     2941 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaat tgtgaccccg
     3001 atacgacaag cgacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     3061 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3121 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3181 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3241 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3301 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3361 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3421 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3481 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3541 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3601 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3661 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3721 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3901 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3961 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4021 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4081 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4261 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4321 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4381 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4441 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4501 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4861 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4921 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6961 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7081 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7141 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7201 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7261 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7321 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7381 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7441 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7501 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7561 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7621 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7681 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7741 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7801 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7861 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7921 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7981 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8041 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8101 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8161 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8221 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8281 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8341 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8401 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8461 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8521 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8581 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8641 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8701 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8761 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8821 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8881 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8941 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     9001 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9061 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9121 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9181 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9241 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9301 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9361 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9421 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9481 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9541 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9601 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9661 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9721 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     9781 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     9841 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9901 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaa
//
