LOCUS       sequence-380284-        8917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of CALM2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX317 CALM2
FEATURES             Location/Qualifiers
     source          1..8917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        138..1316
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          369..1307
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1264..1284
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1336..1360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1360..1806
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPT
                     EAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAA
                     ELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     protein_bind    complement(1808..1832)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1840..1881
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1992..2580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2045..2065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2463..2474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2652..2885
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2963..3084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3000..3019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3054..3073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3124..3259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3279..3298)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3280..3298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3308..3325)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3308..3324)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3317..3339)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3466..3921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3553..3572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3763..3784
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3947..4051
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4052..4120,4121..4912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4270..4289)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5083..5671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5572..5591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5825..5842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5959..5980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5995..6000,6001..6018,6019..6025)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6033..6049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6038..6060
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6057..6073
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6057..6073
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6092..6112
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6094..6112
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6140..6366
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6367..6547
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6594..6719
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7212..7445
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7630..7674
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7905..8115
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7927..8123
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7974..8109
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8155..8757
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8155..8174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8651..8671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8800..8917
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc
       61 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
      121 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
      181 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
      241 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
      301 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
      361 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
      421 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
      481 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
      541 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
      601 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
      661 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
      721 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
      781 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
      841 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
      901 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
      961 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     1021 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     1081 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     1141 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     1201 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     1261 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggct
     1321 agcatcgatt gatcaacaag tttgtacaaa aaagttggca tggctgacca actgactgaa
     1381 gagcagattg cagaattcaa agaagctttt tcactatttg acaaagatgg tgatggaact
     1441 ataacaacaa aggaattggg aactgtaatg agatctcttg ggcagaatcc cacagaagca
     1501 gagttacagg acatgattaa tgaagtagat gctgatggta atggcacaat tgacttccct
     1561 gaatttctga caatgatggc aagaaaaatg aaagacacag acagtgaaga agaaattaga
     1621 gaagcattcc gtgtgtttga taaggatggc aatggctata ttagtgctgc agaacttcgc
     1681 catgtgatga caaaccttgg agagaagtta acagatgaag aagttgatga aatgatcagg
     1741 gaagcagata ttgatggtga tggtcaagta aactatgaag agtttgtaca aatgatgaca
     1801 gcaaagtgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1861 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1921 tcttggcttt atatatcttg tggaaaggac gaggatcatg atactattcg cctactacgc
     1981 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2041 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2101 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2161 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2221 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2281 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2341 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2401 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2461 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2521 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2581 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2641 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2701 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2761 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2821 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2881 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     2941 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3061 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3121 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3181 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3241 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3421 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3721 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3781 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3841 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3901 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3961 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4021 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4081 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4141 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4201 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4261 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4321 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4381 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4441 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4501 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4561 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4621 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4681 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4741 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4801 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4861 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4921 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4981 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5041 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5101 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5161 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5221 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5281 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5341 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5401 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5461 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5521 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5581 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5641 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5701 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5761 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5821 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5881 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5941 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6001 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6061 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6121 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6181 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6241 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6301 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6361 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6421 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6481 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6541 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6601 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6661 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6721 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6781 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6841 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     6901 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     6961 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7021 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7081 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7141 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7201 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7261 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7321 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7381 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7441 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7501 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7561 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7621 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7681 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7741 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7801 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc
     7861 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7921 aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7981 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8041 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8101 ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc
     8161 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8221 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8281 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8341 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
     8401 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8461 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8521 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8581 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     8641 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8701 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga
     8761 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     8821 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     8881 agaattacaa aaacaaatta caaaaattca aaatttt
//
