LOCUS       sequence-380287-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of PRDM14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX317 PRDM14
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..1264
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          317..1255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1212..1232
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1284..1308
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3022..3046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3054..3095
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3206..3794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3259..3279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3677..3688)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3866..4099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4177..4298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4214..4233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4268..4287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4338..4473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4493..4512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4494..4512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4522..4539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4522..4538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4531..4553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4680..5135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4767..4786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4977..4998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5161..5265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5266..5334,5335..6126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5484..5503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6297..6885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6786..6805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7039..7056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7173..7194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7209..7214,7215..7232,7233..7239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7247..7263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7252..7274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7271..7287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7271..7287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7306..7326
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7308..7326
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7354..7580
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7581..7761
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7808..7933
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8426..8659
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8844..8888
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9119..9329
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9141..9337
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9188..9323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9369..9971
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9369..9388)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9865..9885
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10014..10131
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
       61 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
      121 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
      181 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      241 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      301 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      361 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcatg gctctacccc
     1321 ggccaagtga ggccgtgcct caggacaagg tgtgctaccc gccggagagc agcccgcaga
     1381 acctggccgc gtactacacg cctttcccgt cctatggaca ctacagaaac agcctggcca
     1441 ccgtggagga agacttccaa cctttccggc agctggaggc cgcagcgtct gctgcccccg
     1501 ccatgccccc cttccccttc cggatggcgc ctcccttgct gagcccgggt ctgggcctac
     1561 agagggagcc tctctacgat ctgccctggt acagcaagct gccaccgtgg tacccaattc
     1621 cccacgtccc cagggaagtg ccgcccttcc tgagcagcag ccacgagtac gcgggtgcca
     1681 gcagtgaaga tctgggccac caaatcattg gtggcgacaa cgagagtggc ccgtgttgtg
     1741 gacctgacac tttaattcca ccgccccctg cggatgcttc tctgttacct gaggggctga
     1801 ggacctccca gttattacct tgctcaccca gcaagcagtc agaggatggt cccaaaccct
     1861 ccaaccaaga agggaagtcc cctgctcggt tccagttcac ggaggaggac ctgcacttcg
     1921 ttctgtacgg ggtcactccc agcctggagc acccagccag cctgcaccat gcgatttcag
     1981 gcctcctggt ccccccagac agctctggat ctgattctct tcctcaaact ctggatgaag
     2041 actcccttca acttccagaa ggtctatgcc tcatgcagac ggtgtttggt gaagtcccac
     2101 attttggtgt gttctgcagt agttttatcg ccaaaggagt caggtttggg ccctttcaag
     2161 gtaaagtggt caatgccagt gaagtgaaga cctacggaga caattctgtg atgtgggaga
     2221 tctttgaaga tggtcatttg agccacttta tagatggaaa aggaggtacg gggaactgga
     2281 tgtcctatgt caactgtgcc cgcttcccca aggagcagaa cctagttgct gtgcagtgtc
     2341 aagggcatat attttatgag agctgcaaag agattcatca gaaccaagag ctccttgtgt
     2401 ggtatggaga ctgctatgag aaatttctgg atattcctgt gagccttcag gtcacagagc
     2461 cggggaagca gccatctggg ccctctgaag agtctgcaga aggctacaga tgtgaaagat
     2521 gtgggaaggt atttacctac aaatattaca gagataagca cctcaagtac accccctgtg
     2581 tggacaaggg cgataggaaa tttccctgtt ctctctgcaa acgatccttt gagaagcggg
     2641 accggcttcg gatccacatt cttcatgttc atgagaagca ccggcctcac aagtgttcta
     2701 catgtgggaa atgtttctct cagtcttcca gcctaaacaa acacatgcga gtccactctg
     2761 gagacagacc ataccagtgt gtgtattgta ctaagaggtt cacagcctcc agcatactcc
     2821 gcacacacat caggcagcac tccggggaga agcccttcaa atgcaagtac tgtggtaaat
     2881 cttttgcatc ccatgctgcc catgacagcc atgtccggcg ttcacacaag gaggacgatg
     2941 gctgctcatg cagcatctgt gggaaaatct tctcagatca agaaacattc tactcccaca
     3001 tgaagtttca tgaagactac tacccaactt tcttgtacaa agtggttgat atcggtaagc
     3061 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg
     3121 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgataac ttcctaacaa
     3181 tctactaaac acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3241 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3301 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3361 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3421 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3481 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3541 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3601 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3661 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3721 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3781 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     3841 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3901 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4081 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     4141 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4201 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4261 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4441 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     4501 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     4681 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4741 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4801 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4861 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4921 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4981 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5041 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5101 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5161 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5281 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5341 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5401 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5461 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5521 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5581 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5641 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5701 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5761 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5821 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5881 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5941 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6001 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6061 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7141 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7201 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7261 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7321 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7381 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7441 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7501 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     7561 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     7621 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7681 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7741 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     7801 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     7861 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     7921 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     7981 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     8041 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     8101 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8161 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8221 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8281 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8341 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8401 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8461 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8521 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8581 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8641 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8701 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8761 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8821 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8881 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8941 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     9001 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     9061 ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     9121 aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac
     9181 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     9241 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     9301 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg
     9361 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     9421 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     9481 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     9541 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca
     9601 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     9661 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     9721 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     9781 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     9841 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     9901 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     9961 gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa
    10021 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    10081 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    10141 tacagggaca gcagagatcc actttggcgc cggctcgagg cct
//
