LOCUS       sequence-380294-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible knockdown of Yap1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLKO-Tet-On shYap1-1
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    302..323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(338..343,344..361,362..368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    376..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     381..403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     400..416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     400..416
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     435..455
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        437..455
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        483..709
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             710..890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    937..1062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1555..1788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1973..2017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2306..2400
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2317..2336
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2354..2372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2382..2400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2501..2618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2667..3177
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3096..3114
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3186..3758
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3766..3785
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3813..3831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3842..4462
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3915..3934)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4497..5070
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4664..4681)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4891..4910
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5071..5871
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5131..5150)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5741..5760
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5900..5999)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             6143..6376
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6448..6569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6485..6504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6539..6558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6609..6744
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6765..6783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6793..6810)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6793..6809)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6802..6824)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6951..7406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7038..7057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7248..7269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7432..7536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7537..7605,7606..8397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7755..7774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8568..9142,1..14)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9057..9076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
       61 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
      121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
      181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
      241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      421 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
      481 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      601 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      661 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1381 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc
     2281 acgagactag cctcgagcgg ccgcaatatt tgcatgtcgc tatgtgttct gggaaatcac
     2341 cataaacgtg aaatccctat cagtgataga gacttataag ttccctatca gtgatagaga
     2401 caccgggaag cgctgagttc cgaaatcctc gaggatttcg gaactcagcg cttctttttg
     2461 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2521 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2581 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2641 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
     2701 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     2761 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     2821 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     2881 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     2941 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     3001 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     3061 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     3121 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     3181 gatctgtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt
     3241 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc
     3301 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt
     3361 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt
     3421 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac
     3481 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa
     3541 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa
     3601 atattcttat tggtagaaac aactacatcc tggtcatcat cctgcctttc tctttatggt
     3661 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct
     3721 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     3781 ttgtgctgtc tcatcatttt ggcaaagaat tgtaatacga ctcactatag ggcgaattga
     3841 tatgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
     3901 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
     3961 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
     4021 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
     4081 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
     4141 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
     4201 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
     4261 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
     4321 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
     4381 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
     4441 acttaaatgt gaaagtgggt ccgcgtacag cggctcccgg gagttctagg gatctgcccc
     4501 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4561 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4621 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4681 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4741 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4801 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4861 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4921 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     4981 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     5041 gtggttttcc tttgaaaaac acgatgataa ggatccatga ttgaacaaga tggattgcac
     5101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5161 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5221 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     5281 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5341 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5401 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5461 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5521 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5581 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     5641 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     5701 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     5761 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     5821 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggtaccttt
     5881 tttaagggtg ggcgcgccga cccagctttc ttgtacaaag ttggcattat aagaaagcat
     5941 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
     6001 catccagctg atatccgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     6061 ccccatgcat cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6121 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6181 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6241 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6301 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6361 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6421 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6541 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6601 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6721 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6781 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6841 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6901 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6961 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7021 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7081 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7141 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7201 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7261 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7321 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7381 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7561 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7621 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7681 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7741 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7801 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7861 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7921 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7981 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8041 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8101 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8161 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8221 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8281 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8341 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8401 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9121 ttgtgatgct cgtcaggggg gc
//
