LOCUS       sequence-380295-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible knockdown of Yap1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLKO-Tet-On shYap1-4
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     197..214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    331..352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(367..372,373..390,391..397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    405..421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     410..432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     429..445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     429..445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     464..484
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        466..484
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        512..738
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             739..919
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    966..1091
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1584..1817
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2002..2046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2335..2429
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2346..2365
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2383..2401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2411..2429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2530..2647
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2696..3206
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3125..3143
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3215..3787
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3795..3814
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3842..3860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3871..4491
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3944..3963)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4526..5099
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4693..4710)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4920..4939
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5100..5900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5160..5179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5770..5789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5929..6028)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             6172..6405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6477..6598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6514..6533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6568..6587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6638..6773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6794..6812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6822..6839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6822..6838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6831..6853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6980..7435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7067..7086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7277..7298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7461..7565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7566..7634,7635..8426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7784..7803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8597..9142,1..43)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9086..9105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
       61 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      121 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      181 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      241 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      301 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      361 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      421 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      481 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      541 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      601 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      661 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      721 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      901 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      961 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     1021 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     1081 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     1141 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1201 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1261 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1321 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1381 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1441 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1501 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1561 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1621 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1681 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1741 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1801 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1861 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1921 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1981 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     2041 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     2101 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     2161 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2221 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2281 ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggc cgcaatattt
     2341 gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatccctatc agtgatagag
     2401 acttataagt tccctatcag tgatagagac accgggcaga cagattcctt tgttaactcg
     2461 agttaacaaa ggaatctgtc tgctttttga attctcgacc tcgagacaaa tggcagtatt
     2521 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2581 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2641 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgagggggt
     2701 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     2761 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     2821 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     2881 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     2941 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     3001 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     3061 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     3121 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     3181 accgaatcac cgacctctct ccccaggggg atctgtgagt ttggggaccc ttgattgttc
     3241 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     3301 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     3361 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     3421 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     3481 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     3541 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     3601 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     3661 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     3721 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     3781 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     3841 gtaatacgac tcactatagg gcgaattgat atgtctagat tagataaaag taaagtgatt
     3901 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     3961 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     4021 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     4081 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     4141 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     4201 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     4261 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     4321 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     4381 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     4441 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc
     4501 ggctcccggg agttctaggg atctgcccct ctccctcccc cccccctaac gttactggcc
     4561 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4621 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4681 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4741 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4801 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4861 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4921 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4981 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5041 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
     5101 gatccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5161 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5221 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5281 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5341 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5401 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5461 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5521 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5581 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5641 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5701 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5761 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5821 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5881 ttcttgacga gttcttctga ggtacctttt ttaagggtgg gcgcgccgac ccagctttct
     5941 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     6001 ctatcagtca aaataaaatc attatttgcc atccagctga tatccgcccg ccccacgacc
     6061 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggtaccttta agaccaatga
     6121 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6181 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6241 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6301 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6361 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6421 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6601 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6781 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6841 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6901 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6961 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7321 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7381 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     7441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     7561 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7621 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7681 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7741 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7801 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7861 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7921 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7981 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8041 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8101 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     8161 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     8221 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     8281 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     8341 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     8401 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     8461 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     8521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     8581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9121 ttcgccacct ctgacttgag cg
//
