LOCUS       sequence-380296-        9142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet inducible knockdown of Yap1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLKO-Tet-On shYap1-5
FEATURES             Location/Qualifiers
     source          1..9142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     167..184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    301..322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(337..342,343..360,361..367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    375..391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     380..402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     399..415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     399..415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     434..454
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        436..454
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        482..708
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             709..889
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    936..1061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1554..1787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1972..2016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2305..2399
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2316..2335
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2353..2371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2381..2399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2500..2617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2666..3176
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3095..3113
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3185..3757
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3765..3784
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3812..3830
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3841..4461
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3914..3933)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4496..5069
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4663..4680)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4890..4909
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5070..5870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5130..5149)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5740..5759
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    complement(5899..5998)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             6142..6375
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6447..6568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6484..6503)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6538..6557
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6608..6743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6764..6782)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6792..6809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6792..6808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6801..6823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6950..7405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7037..7056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7247..7268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7431..7535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7536..7604,7605..8396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7754..7773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8567..9142,1..13)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9056..9075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
       61 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      301 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      361 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      421 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      481 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      541 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      601 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      661 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      721 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      781 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      841 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      901 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      961 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1021 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1081 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1141 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1201 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1261 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1321 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1381 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1441 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1501 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1561 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1621 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1681 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1741 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1801 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1861 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1921 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1981 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2041 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2101 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2161 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2221 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     2281 cgagactagc ctcgagcggc cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
     2341 ataaacgtga aatccctatc agtgatagag acttataagt tccctatcag tgatagagac
     2401 accggatgtg tctccaggag taataactcg agttattact cctggagaca cattttttga
     2461 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2641 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     2701 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     2761 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     2821 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     2881 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     2941 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     3001 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     3061 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     3121 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     3181 atctgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
     3241 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     3301 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
     3361 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
     3421 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
     3481 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
     3541 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
     3601 tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt
     3661 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
     3721 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     3781 tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg gcgaattgat
     3841 atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
     3901 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
     3961 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
     4021 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
     4081 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
     4141 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
     4201 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
     4261 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
     4321 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
     4381 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
     4441 cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttctaggg atctgcccct
     4501 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4561 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4621 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4681 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4741 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4801 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4861 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4921 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4981 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5041 tggttttcct ttgaaaaaca cgatgataag gatccatgat tgaacaagat ggattgcacg
     5101 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5161 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5221 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5281 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5341 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5401 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5461 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5521 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5581 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5641 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5701 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5761 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5821 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggtacctttt
     5881 ttaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata agaaagcatt
     5941 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     6001 atccagctga tatccgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     6061 cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6121 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6181 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6241 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6301 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6361 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6421 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6541 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6601 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6661 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6721 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6781 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7021 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7141 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7261 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7321 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7381 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7441 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7501 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7561 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7621 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7681 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7741 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7801 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7861 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7921 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7981 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8041 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8101 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8161 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8221 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8281 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8341 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8401 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8461 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8521 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8581 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8641 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8701 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8761 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8821 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8881 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8941 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9001 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9061 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9121 tgtgatgctc gtcagggggg cg
//
