LOCUS       sequence-380315-       10909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of PRDM14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10909)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 10909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX307 P2A GFP PRDM14
FEATURES             Location/Qualifiers
     source          1..10909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     376..396
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    525..642
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        780..1958
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1011..1949
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1906..1926
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1978..2002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3716..3740)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3748..3789
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3799..3855
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3856..3858,3859..3861,3862..4575)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3901..3922)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4162..4181)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4509..4530
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4626..5214
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4679..4699)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5097..5108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5286..5519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5597..5718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5634..5653)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5688..5707
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5758..5893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5913..5932)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5914..5932)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5942..5959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5942..5958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5951..5973)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6100..6555
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6187..6206)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6397..6418
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6581..6685
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6686..6754,6755..7546)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6904..6923)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7717..8305
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8206..8225
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8459..8476
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8593..8614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8629..8634,8635..8652,8653..8659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8667..8683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8672..8694
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8691..8707
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8691..8707
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8726..8746
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8728..8746
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8774..9000
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9001..9181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9228..9353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9846..10079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10264..10308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10539..10749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10561..10757
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10608..10743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(10789..10909,1..482)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10789..10808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
       61 tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga
      121 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
      181 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
      241 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
      301 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
      361 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
      421 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt
      481 aacgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
      541 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
      601 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
      661 agcagagatc cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt
      721 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
      781 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      841 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      901 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      961 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     1021 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     1081 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     1141 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     1201 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     1261 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     1321 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     1381 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     1441 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     1501 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     1561 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     1621 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     1681 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     1741 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1801 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1861 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1921 tggttcaaag tttttttctt ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca
     1981 agtttgtaca aaaaagttgg catggctcta ccccggccaa gtgaggccgt gcctcaggac
     2041 aaggtgtgct acccgccgga gagcagcccg cagaacctgg ccgcgtacta cacgcctttc
     2101 ccgtcctatg gacactacag aaacagcctg gccaccgtgg aggaagactt ccaacctttc
     2161 cggcagctgg aggccgcagc gtctgctgcc cccgccatgc cccccttccc cttccggatg
     2221 gcgcctccct tgctgagccc gggtctgggc ctacagaggg agcctctcta cgatctgccc
     2281 tggtacagca agctgccacc gtggtaccca attccccacg tccccaggga agtgccgccc
     2341 ttcctgagca gcagccacga gtacgcgggt gccagcagtg aagatctggg ccaccaaatc
     2401 attggtggcg acaacgagag tggcccgtgt tgtggacctg acactttaat tccaccgccc
     2461 cctgcggatg cttctctgtt acctgagggg ctgaggacct cccagttatt accttgctca
     2521 cccagcaagc agtcagagga tggtcccaaa ccctccaacc aagaagggaa gtcccctgct
     2581 cggttccagt tcacggagga ggacctgcac ttcgttctgt acggggtcac tcccagcctg
     2641 gagcacccag ccagcctgca ccatgcgatt tcaggcctcc tggtcccccc agacagctct
     2701 ggatctgatt ctcttcctca aactctggat gaagactccc ttcaacttcc agaaggtcta
     2761 tgcctcatgc agacggtgtt tggtgaagtc ccacattttg gtgtgttctg cagtagtttt
     2821 atcgccaaag gagtcaggtt tgggcccttt caaggtaaag tggtcaatgc cagtgaagtg
     2881 aagacctacg gagacaattc tgtgatgtgg gagatctttg aagatggtca tttgagccac
     2941 tttatagatg gaaaaggagg tacggggaac tggatgtcct atgtcaactg tgcccgcttc
     3001 cccaaggagc agaacctagt tgctgtgcag tgtcaagggc atatatttta tgagagctgc
     3061 aaagagattc atcagaacca agagctcctt gtgtggtatg gagactgcta tgagaaattt
     3121 ctggatattc ctgtgagcct tcaggtcaca gagccgggga agcagccatc tgggccctct
     3181 gaagagtctg cagaaggcta cagatgtgaa agatgtggga aggtatttac ctacaaatat
     3241 tacagagata agcacctcaa gtacaccccc tgtgtggaca agggcgatag gaaatttccc
     3301 tgttctctct gcaaacgatc ctttgagaag cgggaccggc ttcggatcca cattcttcat
     3361 gttcatgaga agcaccggcc tcacaagtgt tctacatgtg ggaaatgttt ctctcagtct
     3421 tccagcctaa acaaacacat gcgagtccac tctggagaca gaccatacca gtgtgtgtat
     3481 tgtactaaga ggttcacagc ctccagcata ctccgcacac acatcaggca gcactccggg
     3541 gagaagccct tcaaatgcaa gtactgtggt aaatcttttg catcccatgc tgcccatgac
     3601 agccatgtcc ggcgttcaca caaggaggac gatggctgct catgcagcat ctgtgggaaa
     3661 atcttctcag atcaagaaac attctactcc cacatgaagt ttcatgaaga ctactaccca
     3721 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     3781 gattctacgg gcagcggcgc cacaaacttc tctctgctaa agcaagcagg tgatgttgaa
     3841 gaaaaccccg ggcctatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     3901 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     3961 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4021 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
     4081 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4141 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4201 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4261 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
     4321 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
     4381 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4441 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     4501 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     4561 gagctgtaca agtaatagta atgaactagt accggttaag tcgacaatca acgcgttaag
     4621 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4681 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4741 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4801 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4861 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4921 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4981 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5041 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5101 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5161 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     5221 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5281 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5521 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     5581 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5641 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5701 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5821 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5881 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     5941 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6001 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6061 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6121 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6181 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6241 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6301 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6361 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6421 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6481 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6541 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6601 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     6661 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     6721 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6781 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6841 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6901 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6961 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7021 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7081 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7141 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7201 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7261 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7321 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7381 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7441 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7501 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7561 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7621 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7681 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7741 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7801 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7861 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7921 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7981 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8041 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8101 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8161 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8221 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8281 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8341 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8401 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8461 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8521 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     8581 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     8641 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     8701 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     8761 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     8821 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     8881 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     8941 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     9001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     9421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     9481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     9541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     9601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     9661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     9721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     9781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     9841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     9901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     9961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
    10501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat gaccgagtac
    10801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggacc
//
