LOCUS       sequence-380326-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A2 Proximal promoter sequence consisting of the target sequence for 
            the gRNA1 (GB1838) at ""e site"" (-320 from TSS). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3273
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     236..255
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     483..501
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(505..548)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    551..572
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      894..951
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(975..992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1146..1734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1226..1245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1917..2011)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2032..2370,1..321))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
       61 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
      121 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
      181 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
      241 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
      301 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
      361 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc
      421 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      481 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
      541 atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcgtga cccgtcttct
      601 ctcaccaacc agtcatacct ggagctgtac cgttattgcg ctgcatagat gcagtgctgc
      661 tcttatcaca tttgtttcga cgacagccgc cttcgcagtt tcctcagaca cttaagaata
      721 agcgcttatt gtaggcagag gcacgcccta ttagtggctg cggcaaaata tcttctaagc
      781 gaattcctgt gctcggtggt caaatggtat cgtggctgcg taatcagccg tatccagcaa
      841 cactacgcta tctccctgag accactcatc gcgactagta gcggccgctg cagtccggca
      901 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      961 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1141 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
     1921 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1981 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     2041 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     2101 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     2161 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     2221 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
     2281 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2341 tagaaactgc cggaaatcgt cgtggtattc
//
